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molecular cloning

"Strategy for the analysis of tissue-specific methylation changes without physical isolation" (2019) Beyrne, C.C.; González, R.M.; Iusem, N.D. Epigenetics. 14(1):41-51

"Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth" (2018) Sede, A.R.;Borassi, C.;Wengier, D.L. (...)Muschietti, J.P. FEBS Letters. 592(2):233-243

"Efficient production of glycosylated and non-glycosylated mycobacterium tuberculosis antigen 85B fused to PVX coat protein in Nicotiana benthamiana plants" (2018) González, P.A.; Puccio, F.D.; Zelada, A.M. American Journal of Biochemistry and Biotechnology. 14(4):238-246

"The Phosphatidylinositol 3-kinase Class III Complex Containing TcVps15 and TcVps34 Participates in Autophagy in Trypanosoma cruzi" (2017) Schoijet, A.C.; Sternlieb, T.; Alonso, G.D. Journal of Eukaryotic Microbiology. 64(3):308-321

"Autocrine IL-6 mediates pituitary tumor senescence" (2017) Sapochnik, M.;Haedo, M.R.;Fuertes, M. (...)Arzt, E. Oncotarget. 8(3):4690-4702

"Expression and refolding of the protective antigen of Bacillus anthracis: A model for high-throughput screening of antigenic recombinant protein refolding " (2016) Pavan, M.E.; Pavan, E.E.; Cairó, F.M.; Pettinari, M.J. Revista Argentina de Microbiologia. 48(1):5-14

"Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress" (2016) Prymaczok, N.C.;Pasqualino, V.M.;Viau, V.E. (...)Medesani, D.A. Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology. 186(2):181-191

"PrxQ B from Mycobacterium tuberculosis is a monomeric, thioredoxin-dependent and highly efficient fatty acid hydroperoxide reductase" (2016) Reyes, A.M.;Vazquez, D.S.;Zeida, A. (...)Trujillo, M. Free Radical Biology and Medicine. 101:249-260

"The estrogen receptor alpha nuclear localization sequence is critical for fulvestrant-induced degradation of the receptor" (2015) Casa, A.J.;Hochbaum, D.;Sreekumar, S. (...)Lee, A.V. Molecular and Cellular Endocrinology. 415:76-86

"Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors" (2015) Solari, C.;Echegaray, C.V.;Cosentino, M.S. (...)Guberman, A. PLoS ONE. 10(12)

"Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes" (2015) Gimenez, A.M.;Gesumaría, M.C.;Schoijet, A.C. (...)Machado, E.E. Molecular and Biochemical Parasitology. 203(1-2):14-24

"Translational Fusion and Redirection to Thylakoid Lumen as Strategies to Enhance Accumulation of Human Papillomavirus E7 Antigen in Tobacco Chloroplasts" (2014) Morgenfeld, M.;Lentz, E.;Segretin, M.E. (...)Bravo-Almonacid, F. Molecular Biotechnology. 56(11):1021-1031

"The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing" (2014) Delfosse, V.C.;Agrofoglio, Y.C.;Casse, M.F. (...)Distéfano, A.J. Virus Research. 180:70-75

"Agroinoculation of a full-length cDNA clone of cotton leafroll dwarf virus (CLRDV) results in systemic infection in cotton and the model plant Nicotiana benthamiana" (2013) Delfosse, V.C.;Casse, M.F.;Agrofoglio, Y.C. (...)Distéfano, A.J. Virus Research. 175(1):64-70

"Biophysical Characterisation of Neuroglobin of the Icefish, a Natural Knockout for Hemoglobin and Myoglobin. Comparison with Human Neuroglobin" (2012) Giordano, D.;Boron, I.;Abbruzzetti, S. (...)Verde, C. PLoS ONE. 7(12)

"Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation" (2012) Cánepa, M.M.;Zhu, Y.;Fossati, M. (...)Vissio, P.G. General and Comparative Endocrinology. 176(1):52-61

"Functional Characterization of TLR4 +3725 G/C Polymorphism and Association with Protection against Overweight" (2012) Penas-Steinhardt, A.;Barcos, L.S.;Belforte, F.S. (...)Leskow, F.C. PLoS ONE. 7(12)

"The peculiar heme pocket of the 2/2 hemoglobin of cold-adapted Pseudoalteromonas haloplanktis TAC125" (2011) Howes, B.D.;Giordano, D.;Boechi, L. (...)Smulevich, G. Journal of Biological Inorganic Chemistry. 16(2):299-311

"Functional characterization of methionine sulfoxide reductase A from Trypanosoma spp." (2011) Arias, D.G.;Cabeza, M.S.;Erben, E.D. (...)Guerrero, S.A. Free Radical Biology and Medicine. 50(1):37-46

"StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling" (2011) Giammaria, V.;Grandellis, C.;Bachmann, S. (...)Ulloa, R.M. Planta. 233(3):593-609

"Human recombinant antibodies against Trypanosoma cruzi ribosomal P2β protein" (2011) Grippo, V.; Niborski, L.L.; Gomez, K.A.; Levin, M.J. Parasitology. 138(6):736-747

"Characterization of the beta amyloid precursor protein-like gene in the central nervous system of the crab Chasmagnathus. Expression during memory consolidation" (2010) Fustiñana, M.S.;Ariel, P.;Federman, N. (...)Romano, A. BMC Neuroscience. 11

"Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification" (2010) Figuerola, E.L.M.; Erijman, L. Journal of Hazardous Materials. 181(1-3):281-288

"Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)" (2010) Zavallo, D.; Bilbao, M.L.; Hopp, H.E.; Heinz, R. Plant Cell Reports. 29(3):239-248

"Cloning, functional characterization, and co-expression studies of a novel aquaporin (FaPIP2;1) of strawberry fruit" (2010) Alleva, K.;Marquez, M.;Villarreal, N. (...)Amodeo, G. Journal of Experimental Botany. 61(14):3935-3945

"Genomic and functional characterization of StCDPK1" (2009) Gargantini, P.R.;Giammaria, V.;Grandellis, C. (...)Ulloa, R.M. Plant Molecular Biology. 70(1-2):153-172

"Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in the cold tolerant Patagonian species Bromus pictus" (2009) Del Viso, F.; Puebla, A.F.; Hopp, H.E.; Heinz, R.A. Planta. 231(1):13-25

"Molecular characterization of a putative sucrose:Fructan 6-fructosyltransferase (6-SFT) of the cold-resistant patagonian grass bromus pictus associated with fructan accumulation under low temperatures" (2009) Del Viso, F.;Puebla, A.F.;Fusari, C.M. (...)Heinz, R.A. Plant and Cell Physiology. 50(3):489-503

"Safety assessment of food products from r-DNA animals" (2009) Lema, M.A.; Burachik, M. Comparative Immunology, Microbiology and Infectious Diseases. 32(2):163-189

"Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes" (2009) Mayoral, J.G.;Nouzova, M.;Yoshiyama, M. (...)Noriega, F.G. Insect Biochemistry and Molecular Biology. 39(1):31-37

"Virus infection elevates transcriptional activity of miR164a promoter in plants" (2009) Bazzini, A.A.;Almasia, N.I.;Manacorda, C.A. (...)Asurmendi, S. BMC Plant Biology. 9

"Functional characterization and protein-protein interactions of trypanosome splicing factors U2AF35, U2AF65 and SF1" (2009) Vazquez, M.P.;Mualem, D.;Bercovich, N. (...)Levin, M.J. Molecular and Biochemical Parasitology. 164(2):137-146

"TcPARP: A DNA damage-dependent poly(ADP-ribose) polymerase from Trypanosoma cruzi" (2008) Fernández Villamil, S.H.;Baltanás, R.;Alonso, G.D. (...)Flawiá, M.M. International Journal for Parasitology. 38(3-4):277-287

"Mucor rouxii Rho1 protein; characterization and possible role in polarized growth" (2007) Argimón, S.;Galello, F.;Pereyra, E. (...)Moreno, S. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 91(3):237-251

"Microsatellite typing of ancient maize: Insights into the history of agriculture in southern South America" (2007) Lia, V.V.;Confalonieri, V.A.;Ratto, N. (...)Brown, T.A. Proceedings of the Royal Society B: Biological Sciences. 274(1609):545-554

"Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish" (2007) Rojas, D.A.;Perez-Munizaga, D.A.;Centanin, L. (...)Reyes, A.E. Gene Expression Patterns. 7(3):339-345

"Superinfection exclusion in BHK-21 cells persistently infected with Junín virus" (2007) Ellenberg, P.; Linero, F.N.; Scolaro, L.A. Journal of General Virology. 88(10):2730-2739

"The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence" (2007) Posadas, D.M.;Martín, F.A.;Sabio Y Garcïa, J.V. (...)Zorreguieta, A. Infection and Immunity. 75(1):379-389

"Sequence evolution of the major satellite DNA of the genus Ctenomys (Octodontidae, Rodentia)" (2007) Ellingsen, A.; Slamovits, C.H.; Rossi, M.S. Gene. 392(1-2):283-290

"Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus" (2007) Guzmán, F.A.;Distéfano, A.J.;Arneodo, J.D. (...)Conci, L.R. Archives of Virology. 152(3):565-573

"A yeast sterol carrier protein with fatty-acid and fatty-acyl-CoA binding activity" (2006) Ferreyra, R.G.;Burgardt, N.I.;Milikowski, D. (...)Ermácora, M.R. Archives of Biochemistry and Biophysics. 453(2):197-206

"Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene" (2006) Ayub, N.D.; Julia Pettinari, M.; Méndez, B.S.; López, N.I. FEMS Microbiology Letters. 264(1):125-131

"Multiple roles of the F-box protein Slimb in Drosophila egg chamber development" (2005) Mussopappa, M.; Wappner, P. Development. 132(11):2561-2571

"Sequence analysis of genome segments S5 and S10 of Mal de Río Cuarto virus (Fijivirus, Reoviridae). Brief Report" (2005) Distéfano, A.J.; Hopp, H.E.; Del Vas, M. Archives of Virology. 150(6):1241-1248

"Stoichiometry of the α9α10 nicotinic cholinergic receptor" (2005) Plazas, P.V.;Katz, E.;Gomez-Casati, M.E. (...)Elgoyhen, A.B. Journal of Neuroscience. 25(47):10905-10912

"Identification and characterisation of the α and β subunits of succinyl CoA ligase of tomato" (2005) Studart-Guimarães, C.;Gibon, Y.;Frankel, N. (...)Carrari, F. Plant Molecular Biology. 59(5):781-791

"A novel α-conotoxin, PeIA, cloned from Conus pergrandis, discriminates between Rat α9α10 and α7 nicotinic cholinergic receptors" (2005) McIntosh, J.M.;Plazas, P.V.;Watkins, M. (...)Elgoyhen, A.B. Journal of Biological Chemistry. 280(34):30107-30112

"Protein kinase A is involved in the control of morphology and branching during aerobic growth of Mucor circinelloides" (2004) Lübbehüsen, T.;González Polo, V.;Rossi, S. (...)Arnau, J. Microbiology. 150(1):143-150

"Regulation of Drosophila hypoxia-inducible factor (HIF) activity in SL2 cells: Identification of a hypoxia-induced variant isoform of the HIFα homolog gene similar" (2004) Gorr, T.A.; Tomita, T.; Wappner, P.; Bunn, H.F. Journal of Biological Chemistry. 279(34):36048-36058

"Involvement of the gp130 cytokine transducer in MtT/S pituitary somatotroph tumour development in an autocrine-paracrine model" (2004) Graciarena, M.;Carbia-Nagashima, A.;Onofri, C. (...)Arzt, E. European Journal of Endocrinology. 151(5):595-604

"Mepyramine, a histamine H1 receptor inverse agonist, binds preferentially to a G protein-coupled form of the receptor and sequesters G protein" (2004) Fitzsimons, C.P.;Monczor, F.;Fernández, N. (...)Davio, C. Journal of Biological Chemistry. 279(33):34431-34439

"Differential representation of sunflower ESTs in enriched organ-specific cDNA libraries in a small scale sequencing project" (2003) Fernández, P.;Paniego, N.;Lew, S. (...)Heinz, R.A. BMC Genomics. 4

"Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins" (2003) López Bergami, P.;Mateos, P.;Hoebeke, J. (...)Baldi, A. Biochemical and Biophysical Research Communications. 301(4):819-824

"Inhibitory effect of AP-1 complex on 5-aminolevulinate synthase gene expression through sequestration of cAMP-response element protein (CRE)-binding protein (CBP) coactivator" (2003) Guberman, A.S.;Scassa, M.E.;Giono, L.E. (...)Cánepa, E.T. Journal of Biological Chemistry. 278(4):2317-2326

"cDNA cloning, biochemical and phylogenetic characterization of β- and β' -subunits of Candida albicans protein kinase CK2" (2003) Zelada, A.;De Souza, F.S.J.;Walz, K. (...)Passeron, S. Yeast. 20(6):471-478

"Unique features of the Trypanosoma cruzi U2AF35 splicing factor*" (2003) Vázquez, M.;Atorrasagasti, C.;Bercovich, N. (...)Levin, M.J. Molecular and Biochemical Parasitology. 128(1):77-81

"Adaptive Evolution of the Water Stress-Induced Gene Asr2 in Lycopersicon Species Dwelling in Arid Habitats" (2003) Frankel, N.; Hasson, E.; Iusem, N.D.; Rossi, M.S. Molecular Biology and Evolution. 20(12):1955-1962

"Phosphoenolpyruvate phosphotransferase system and N-acetylglucosamine metabolism in Bacillus sphaericus" (2003) Alice, A.F.; Pérez-Martínez, G.; Sánchez-Rivas, C. Microbiology. 149(7):1687-1698

"Null mutations in the essential gene yrfF (mucM) are not lethal in rcsB, yojN or rcsC strains of Salmonella enterica serovar Typhimurium" (2003) Costa, C.S.; Pettinari, M.J.; Méndez, B.S.; Antón, D.N. FEMS Microbiology Letters. 222(1):25-32

"A refined molecular karyotype for the reference strain of the Trypanosoma cruzi genome project (clone CL Brener) by assignment of chromosome markers" (2003) Porcile, P.E.;Santos, M.R.M.;Souza, R.T. (...)Da Silveira, J.F. Gene. 308(1-2):53-65

"A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants" (2002) Chico, J.M.; Raíces, M.; Téllez-Iñón, M.T.; Ulloa, R.M. Plant Physiology. 128(1):256-270

"Rat kidney porphobilinogen deaminase kinetics: Detection of enzyme-substrate complexes" (2002) Noriega, G.; Mattei, G.; Batlle, A.; Juknat, A.A. International Journal of Biochemistry and Cell Biology. 34(10):1230-1240

"A novel calcium-stimulated adenylyl cyclase from Trypanosoma cruzi, which interacts with the structural flagellar protein paraflagellar rod" (2002) D'Angelo, M.A.;Montagna, A.E.;Sanguineti, S. (...)Flawiá, M.M. Journal of Biological Chemistry. 277(38):35025-35034

"Existence of a true phosphofructokinase in Bacillus sphaericus: Cloning and sequencing of the pfk gene" (2002) Alice, A.F.; Pérez-Martínez, G.; Sáchez-Rivas, C. Applied and Environmental Microbiology. 68(12):6410-6415

"Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8" (2001) Pettinari, M.J.;Vázquez, G.J.;Silberschmidt, D. (...)Méndez, B.S. Applied and Environmental Microbiology. 67(3-12):5331-5334

"α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells" (2001) Elgoyhen, A.B.;Vetter, D.E.;Katz, E. (...)Boulter, J. Proceedings of the National Academy of Sciences of the United States of America. 98(6):3501-3506

"T cell vaccination in secondary progressive multiple sclerosis" (2000) Correale, J.;Lund, B.;McMillan, M. (...)Weiner, L.P. Journal of Neuroimmunology. 107(2):130-139

"Screening for and characterization of phospholipase A1 hypersecretory mutants of Tetrahymena thermophila" (2000) Hartmann, M.; Guberman, A.; Florin-Christensen, M.; Tiedtke, A. Applied Microbiology and Biotechnology. 54(3):390-396

"Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites" (2000) Pereira, C.A.;Alonso, G.D.;Paveto, M.C. (...)Flawiá, M.M. Journal of Biological Chemistry. 275(2):1495-1501

"Expression and biochemical characterisation of recombinant AceA, a bacterial α-mannosyltransferase" (1999) Geremia, R.A.;Roux, M.;Ferreiro, D.U. (...)Ielpi, L. Molecular and General Genetics. 261(6):933-940

"Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins" (1998) Gómez, E.B.; Kornblihtt, A.R.; Téllez-Iñón, M.T. Molecular and Biochemical Parasitology. 91(2):337 -351

"Expression of a recombinant Fab antibody fragment against cruzipain, the major cysteine proteinase of Trypanosoma cruzi" (1998) Kaplan, D.;Baldi, C.;Chiaramonte, M.G. (...)Baldi, A. Biochemical and Biophysical Research Communications. 253(1):53-58

"Genetic heterogeneity of autosomal dominant polycystic kidney disease in Argentina" (1997) Iglesias, D.M.;Martín, R.S.;Fraga, A. (...)Bernath, V. Journal of Medical Genetics. 34(10):827-830

"Cloning of two alternatively spliced 21-hydroxylase CDNAs from rat adrenal" (1997) Zhou, M.-Y.; Del Carmen Vila, M.; Gomez-Sanchez, E.P.; Gomez-Sanchez, C.E. Journal of Steroid Biochemistry and Molecular Biology. 62(4):277-286

"B21 anti-breast Ca" (1997) Baldi, A. Hybridoma. 16(3):302

"A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi" (1996) Buscaglia, C.A.;Pollevick, G.D.;Veloso, C. (...)Sánchez, D.O. Biochimica et Biophysica Acta - Gene Structure and Expression. 1309(1-2):53-57

"Interspecies cross-reactivity of a monoclonal antibody directed against wheat chloroplast fructose-1,6-bisphosphatase." (1996) Hagelin, K.;Rodriguez-Suarez, R.;Katzen, F. (...)Dyer, T. Cellular and molecular biology (Noisy-le-Grand, France). 42(5):673-682

"Cloning and sequence analysis of the TcP2β cDNA variants of Trypanosoma cruzi" (1995) Schijman, A.G.;Vazquez, M.P.;Dov, C.B. (...)Levin, M.J. BBA - Gene Structure and Expression. 1264(1):15-18

"Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria" (1995) Matkovic, L.; Gomez-Sanchez, C.E.; Lantos, C.P.; Cozza, E.N. Steroids. 60(6):447-452

"A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities" (1995) Montemartini, M.;Búa, J.;Bontempi, E. (...)Nowicki, C. FEMS Microbiology Letters. 133(1-2):17-20

"Endothelin-1 stimulation of aldosterone and zona glomerulosa ouabain-sensitive sodium/potassium-ATPase" (1994) Pecci, A.;Cozza, E.N.;Devlin, M. (...)Gomez-Sanchez, E.P. Journal of Steroid Biochemistry and Molecular Biology. 50(1-2):49-53

"Identification, genetic and biochemical analysis of genes involved in synthesis of sugar nucleotide precursors of xanthan gum" (1993) Harding, N.E.;Raffo, S.;Raimondi, A. (...)Ielpi, L. Journal of General Microbiology. 139(3):447-457

"The chronic presence of the parasite, and anti-P autoimmunity in Chagas disease: The Trypanosoma cruzi ribosomal P proteins, and their recognition by the host immune system" (1993) Kaplan, D.;Vazquez, M.;Lafon, S. (...)Levin, M.J. Biological Research. 26(1-2):273-277

"Nucleotide sequence of a cDNA encoding a Trypanosoma cruzi acidic ribosomal PO protein: A novel C-terminal domain in T.cruzi ribosomal P proteins" (1992) Schijman, A.G.; Levin, M.J. Nucleic Acids Research. 20(11):2894

"Location and cloning of the ketal pyruvate transferase gene of Xanthomonas campestris" (1991) Marzocca, M.P.;Harding, N.E.;Petroni, E.A. (...)Ielpi, L. Journal of Bacteriology. 173(23):7519-7524

"Identification of the product of an Agrobacterium tumefaciens chromosomal virulence gene." (1988) Zorreguieta, A.;Geremia, R.A.;Cavaignac, S. (...)Ugalde, R.A. Molecular plant-microbe interactions : MPMI. 1(3):121-127