Artículo

Gargantini, P.R.; Giammaria, V.; Grandellis, C.; Feingold, S.E.; Maldonado, S.; Ulloa, R.M. "Genomic and functional characterization of StCDPK1" (2009) Plant Molecular Biology. 70(1-2):153-172
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Abstract:

StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is located in the distal portion of chromosome 12. Western blot and immunolocalization assays (using confocal and transmission electron microscopy) performed with a specific antibody against StCDPK1 indicate that this kinase is mainly located in the plasma membrane of swelling stolons and sprouting tubers. Sucrose (4-8%) increased StCDPK1 protein content in non-induced stolons, however the amount detected in swelling stolons was higher. Transgenic lines with reduced expression of StCDPK1 (β7) did not differ from controls when cultured under multiplication conditions, but when grown under tuber inducing conditions some significant differences were observed: the β7 line tuberized earlier than controls without the addition of CCC (GA inhibitor), developed more tubers than wild type plants in the presence of hormones that promote tuberization in potato (ABA and BAP) and was more insensitive to GA action (stolons were significantly shorter than those of control plants). StCDPK1 expression was induced by GA, ABA and BAP. Our results suggest that StCDPK1 plays a role in GA-signalling and that this kinase could be a converging point for the inhibitory and promoting signals that influence the onset of potato tuberization. © 2009 Springer Science+Business Media B.V.

Registro:

Documento: Artículo
Título:Genomic and functional characterization of StCDPK1
Autor:Gargantini, P.R.; Giammaria, V.; Grandellis, C.; Feingold, S.E.; Maldonado, S.; Ulloa, R.M.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, piso 2, Buenos Aires 1428, Argentina
Laboratorio de Agro-Biotecnología, INTA-EEA Balcarce, CC276, Balcarce 7620, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina
Departamento de Bioquímica, Universidade de São Paulo, Sao Paulo, Brazil
Palabras clave:Calcium-dependent protein kinases; Gibberellins; Solanum tuberosum; Tuberization; calcium binding protein; CDPK1 protein, plant; gibberellin; phytohormone; plant DNA; protein kinase; vegetable protein; article; chromosome map; genetics; metabolism; molecular cloning; plant tuber; potato; transgenic plant; Calcium-Binding Proteins; Chromosome Mapping; Cloning, Molecular; DNA, Plant; Gibberellins; Plant Growth Regulators; Plant Proteins; Plant Tubers; Plants, Genetically Modified; Protein Kinases; Solanum tuberosum; Arabidopsis; Solanum tuberosum; Triticum aestivum; Tuber (truffle)
Año:2009
Volumen:70
Número:1-2
Página de inicio:153
Página de fin:172
DOI: http://dx.doi.org/10.1007/s11103-009-9462-5
Título revista:Plant Molecular Biology
Título revista abreviado:Plant. Mol. Biol.
ISSN:01674412
CODEN:PMBID
CAS:protein kinase, 9026-43-1; CDPK1 protein, plant; Calcium-Binding Proteins; DNA, Plant; Gibberellins; Plant Growth Regulators; Plant Proteins; Protein Kinases, 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674412_v70_n1-2_p153_Gargantini

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Citas:

---------- APA ----------
Gargantini, P.R., Giammaria, V., Grandellis, C., Feingold, S.E., Maldonado, S. & Ulloa, R.M. (2009) . Genomic and functional characterization of StCDPK1. Plant Molecular Biology, 70(1-2), 153-172.
http://dx.doi.org/10.1007/s11103-009-9462-5
---------- CHICAGO ----------
Gargantini, P.R., Giammaria, V., Grandellis, C., Feingold, S.E., Maldonado, S., Ulloa, R.M. "Genomic and functional characterization of StCDPK1" . Plant Molecular Biology 70, no. 1-2 (2009) : 153-172.
http://dx.doi.org/10.1007/s11103-009-9462-5
---------- MLA ----------
Gargantini, P.R., Giammaria, V., Grandellis, C., Feingold, S.E., Maldonado, S., Ulloa, R.M. "Genomic and functional characterization of StCDPK1" . Plant Molecular Biology, vol. 70, no. 1-2, 2009, pp. 153-172.
http://dx.doi.org/10.1007/s11103-009-9462-5
---------- VANCOUVER ----------
Gargantini, P.R., Giammaria, V., Grandellis, C., Feingold, S.E., Maldonado, S., Ulloa, R.M. Genomic and functional characterization of StCDPK1. Plant. Mol. Biol. 2009;70(1-2):153-172.
http://dx.doi.org/10.1007/s11103-009-9462-5