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Abstract:

Background: Human β-amyloid, the main component in the neuritic plaques found in patients with Alzheimer's disease, is generated by cleavage of the β-amyloid precursor protein. Beyond the role in pathology, members of this protein family are synaptic proteins and have been associated with synaptogenesis, neuronal plasticity and memory, both in vertebrates and in invertebrates. Consolidation is necessary to convert a short-term labile memory to a long-term and stable form. During consolidation, gene expression and de novo protein synthesis are regulated in order to produce key proteins for the maintenance of plastic changes produced during the acquisition of new information.Results: Here we partially cloned and sequenced the beta-amyloid precursor protein like gene homologue in the crab Chasmagnathus (cappl), showing a 37% of identity with the fruit fly Drosophila melanogaster homologue and 23% with Homo sapiens but with much higher degree of sequence similarity in certain regions. We observed a wide distribution of cappl mRNA in the nervous system as well as in muscle and gills. The protein localized in all tissues analyzed with the exception of muscle. Immunofluorescence revealed localization of cAPPL in associative and sensory brain areas. We studied gene and protein expression during long-term memory consolidation using a well characterized memory model: the context-signal associative memory in this crab species. mRNA levels varied at different time points during long-term memory consolidation and correlated with cAPPL protein levels. Conclusions: cAPPL mRNA and protein is widely distributed in the central nervous system of the crab and the time course of expression suggests a role of cAPPL during long-term memory formation. © 2010 Fustiñana et al; licensee BioMed Central Ltd.

Registro:

Documento: Artículo
Título:Characterization of the beta amyloid precursor protein-like gene in the central nervous system of the crab Chasmagnathus. Expression during memory consolidation
Autor:Fustiñana, M.S.; Ariel, P.; Federman, N.; Freudenthal, R.; Romano, A.
Filiación:Laboratorio de Neurobiología de la Memoria, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. IFIByNE, CONICET. Ciudad Universitaria, Pab. II, 2do piso (1428EHA), Buenos Aires, Argentina
Palabras clave:amyloid precursor protein; beta amyloid precursor protein like protein; messenger RNA; unclassified drug; amyloid precursor protein; complementary DNA; messenger RNA; amino acid sequence; amino terminal sequence; animal experiment; animal tissue; antenna I neuropil; antenna II nerve; article; association cortex; associative memory; brain region; carboxy terminal sequence; central nervous system; Chasmagnathus granulatus; ciliary ganglion; controlled study; crab; cranial nerve; deutocerebrum; Drosophila melanogaster; gene expression profiling; gene sequence; gill; glycosylation; immunofluorescence; innervation; long term memory; male; memory consolidation; model; molecular cloning; molecular weight; muscle; neuropil; nonhuman; nucleotide sequence; oculomotor nerve; protein domain; protein expression; protein localization; protocerebrum; sensory cortex; sequence homology; supraesophageal ganglion; tegumentary nerve; thoracic ganglion; tissue distribution; tritocerebrum; animal; biosynthesis; Brachyura; central nervous system; genetics; immunohistochemistry; immunoprecipitation; memory; metabolism; molecular genetics; physiology; reverse transcription polymerase chain reaction; Western blotting; Amino Acid Sequence; Amyloid beta-Protein Precursor; Animals; Base Sequence; Blotting, Western; Brachyura; Central Nervous System; Cloning, Molecular; DNA, Complementary; Immunohistochemistry; Immunoprecipitation; Male; Memory; Molecular Sequence Data; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger
Año:2010
Volumen:11
DOI: http://dx.doi.org/10.1186/1471-2202-11-109
Título revista:BMC Neuroscience
Título revista abreviado:BMC Neurosci.
ISSN:14712202
CODEN:BNMEA
CAS:Amyloid beta-Protein Precursor; DNA, Complementary; RNA, Messenger
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_14712202_v11_n_p_Fustinana.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712202_v11_n_p_Fustinana

Referencias:

  • Lorenzo, A., Yuan, M., Zhang, Z., Paganetti, P.A., Sturchler-Pierrat, C., Staufenbiel, M., Mautino, J., Yankner, B.A., Amyloid beta interacts with the amyloid precursor protein: a potential toxic mechanism in Alzheimer's disease (2000) Nat Neurosci, 3 (5), pp. 460-464. , 10.1038/74833, 10769385
  • Selkoe, D.J., Alzheimer's disease is a synaptic failure (2002) Science, 298 (5594), pp. 789-791. , 10.1126/science.1074069, 12399581
  • Doyle, E., Bruce, M.T., Breen, K.C., Smith, D.C., Anderton, B., Regan, C.M., Intraventricular infusions of antibodies to amyloid-beta-protein precursor impair the acquisition of a passive avoidance response in the rat (1990) Neurosci Lett, 115 (1), pp. 97-102. , 10.1016/0304-3940(90)90524-D, 2120637
  • Goodman, Y., Mattson, M.P., Secreted forms of beta-amyloid precursor protein protect hippocampal neurons against amyloid beta-peptide-induced oxidative injury (1994) Exp Neurol, 128 (1), pp. 1-12. , 10.1006/exnr.1994.1107, 8070512
  • Goodman, Y., Mattson, M.P., K+ channel openers protect hippocampal neurons against oxidative injury and amyloid beta-peptide toxicity (1996) Brain Res, 706 (2), pp. 328-332. , 10.1016/0006-8993(95)01367-9, 8822377
  • Ishida, A., Furukawa, K., Keller, J.N., Mattson, M.P., Secreted form of beta-amyloid precursor protein shifts the frequency dependency for induction of LTD, and enhances LTP in hippocampal slices (1997) Neuroreport, 8 (9-10), pp. 2133-2137. , 10.1097/00001756-199707070-00009, 9243598
  • Mattson, M.P., Cheng, B., Culwell, A.R., Esch, F.S., Lieberburg, I., Rydel, R.E., Evidence for excitoprotective and intraneuronal calcium-regulating roles for secreted forms of the beta-amyloid precursor protein (1993) Neuron, 10 (2), pp. 243-254. , 10.1016/0896-6273(93)90315-I, 8094963
  • Mucke, L., Masliah, E., Johnson, W.B., Ruppe, M.D., Alford, M., Rockenstein, E.M., Forss-Petter, S., Abraham, C.R., Synaptotrophic effects of human amyloid beta protein precursors in the cortex of transgenic mice (1994) Brain Res, 666 (2), pp. 151-167. , 10.1016/0006-8993(94)90767-6, 7882025
  • Roch, J.M., Masliah, E., Roch-Levecq, A.C., Sundsmo, M.P., Otero, D.A., Veinbergs, I., Saitoh, T., Increase of synaptic density and memory retention by a peptide representing the trophic domain of the amyloid beta/A4 protein precursor (1994) Proc Natl Acad Sci USA, 91 (16), pp. 7450-7454. , 10.1073/pnas.91.16.7450, 44418, 8052602
  • Storey, E., Beyreuther, K., Masters, C.L., Alzheimer's disease amyloid precursor protein on the surface of cortical neurons in primary culture co-localizes with adhesion patch components (1996) Brain Res, 735 (2), pp. 217-231. , 10.1016/0006-8993(96)00608-7, 8911660
  • Teather, L.A., Magnusson, J.E., Chow, C.M., Wurtman, R.J., Environmental conditions influence hippocampus-dependent behaviours and brain levels of amyloid precursor protein in rats (2002) Eur J Neurosci, 16 (12), pp. 2405-2415. , 10.1046/j.1460-9568.2002.02416.x, 12492435
  • Turner, P.R., O'Connor, K., Tate, W.P., Abraham, W.C., Roles of amyloid precursor protein and its fragments in regulating neural activity, plasticity and memory (2003) Prog Neurobiol, 70 (1), pp. 1-32. , 10.1016/S0301-0082(03)00089-3, 12927332
  • Okamoto, T., Takeda, S., Murayama, Y., Ogata, E., Nishimoto, I., Ligand-dependent G protein coupling function of amyloid transmembrane precursor (1995) J Biol Chem, 270 (9), pp. 4205-4208. , 10.1074/jbc.270.49.29224, 7876177
  • De Strooper, B., Annaert, W., Proteolytic processing and cell biological functions of the amyloid precursor protein (2000) J Cell Sci, 113 (PART 11), pp. 1857-1870
  • Rossjohn, J., Cappai, R., Feil, S.C., Henry, A., McKinstry, W.J., Galatis, D., Hesse, L., Masters, C.L., Crystal structure of the N-terminal, growth factor-like domain of Alzheimer amyloid precursor protein (1999) Nat Struct Biol, 6 (4), pp. 327-331. , 10.1038/7562, 10201399
  • Coulson, E.J., Paliga, K., Beyreuther, K., Masters, C.L., What the evolution of the amyloid protein precursor supergene family tells us about its function (2000) Neurochem Int, 36 (3), pp. 175-184. , 10.1016/S0197-0186(99)00125-4, 10676850
  • Soba, P., Eggert, S., Wagner, K., Zentgraf, H., Siehl, K., Kreger, S., Lower, A., Paro, R., Homo- and heterodimerization of APP family members promotes intercellular adhesion (2005) EMBO J, 24 (20), pp. 3624-3634. , 10.1038/sj.emboj.7600824, 1276707, 16193067
  • Walsh, D.M., Klyubin, I., Fadeeva, J.V., Cullen, W.K., Anwyl, R., Wolfe, M.S., Rowan, M.J., Selkoe, D.J., Naturally secreted oligomers of amyloid beta protein potently inhibit hippocampal long-term potentiation in vivo (2002) Nature, 416 (6880), pp. 535-539. , 10.1038/416535a, 11932745
  • Flood, J.F., Morley, J.E., Roberts, E., Amnestic effects in mice of four synthetic peptides homologous to amyloid beta protein from patients with Alzheimer disease (1991) Proc Natl Acad Sci USA, 88 (8), pp. 3363-3366. , 10.1073/pnas.88.8.3363, 51447, 2014256
  • Romano, A., Serafino, A., Krasnowska, E., Ciotti, M.T., Calissano, P., Ruberti, F., Galli, C., Neuronal fibrillogenesis: amyloid fibrils from primary neuronal cultures impair long-term memory in the crab Chasmagnathus (2003) Behav Brain Res, 147 (1-2), pp. 73-82. , 10.1016/S0166-4328(03)00118-9, 14659572
  • Mileusnic, R., Lancashire, C.L., Rose, S.P., The peptide sequence Arg-Glu-Arg, present in the amyloid precursor protein, protects against memory loss caused by A beta and acts as a cognitive enhancer (2004) Eur J Neurosci, 19 (7), pp. 1933-1938. , 10.1111/j.1460-9568.2004.03276.x, 15078567
  • Feld, M., Galli, C., Piccini, A., Romano, A., Effect on memory of acute administration of naturally secreted fibrils and synthetic amyloid-beta peptides in an invertebrate model (2008) Neurobiol Learn Mem, 89 (4), pp. 407-418. , 10.1016/j.nlm.2007.08.011, 17920304
  • Garcia-Osta, A., Alberini, C.M., Amyloid beta mediates memory formation (2009) Learn Mem, 16 (4), pp. 267-272. , 10.1101/lm.1310209, 2661754, 19318468
  • Dawson, G.R., Seabrook, G.R., Zheng, H., Smith, D.W., Graham, S., O'Dowd, G., Bowery, B.J., Chen, H.Y., Age-related cognitive deficits, impaired long-term potentiation and reduction in synaptic marker density in mice lacking the beta-amyloid precursor protein (1999) Neuroscience, 90 (1), pp. 1-13. , 10.1016/S0306-4522(98)00410-2, 10188929
  • Rosen, D.R., Martin-Morris, L., Luo, L.Q., White, K., A Drosophila gene encoding a protein resembling the human beta-amyloid protein precursor (1989) Proc Natl Acad Sci USA, 86 (7), pp. 2478-2482. , 10.1073/pnas.86.7.2478, 286936, 2494667
  • Luo, L.Q., Martin-Morris, L.E., White, K., Identification, secretion, and neural expression of APPL, a Drosophila protein similar to human amyloid protein precursor (1990) J Neurosci, 10 (12), pp. 3849-3861
  • Swanson, T.L., Knittel, L.M., Coate, T.M., Farley, S.M., Snyder, M.A., Copenhaver, P.F., The insect homologue of the amyloid precursor protein interacts with the heterotrimeric G protein Go alpha in an identified population of migratory neurons (2005) Dev Biol, 288 (1), pp. 160-178. , 10.1016/j.ydbio.2005.09.029, 2862231, 16229831
  • Satpute-Krishnan, P., DeGiorgis, J.A., Conley, M.P., Jang, M., Bearer, E.L., A peptide zipcode sufficient for anterograde transport within amyloid precursor protein (2006) Proc Natl Acad Sci USA, 103 (44), pp. 16532-16537. , 10.1073/pnas.0607527103, 1621108, 17062754
  • Daigle, I., Li, C., Apl-1, a Caenorhabditis elegans gene encoding a protein related to the human beta-amyloid protein precursor (1993) Proc Natl Acad Sci USA, 90 (24), pp. 12045-12049. , 10.1073/pnas.90.24.12045, 48122, 8265668
  • Torroja, L., Packard, M., Gorczyca, M., White, K., Budnik, V., The Drosophila beta-amyloid precursor protein homolog promotes synapse differentiation at the neuromuscular junction (1999) J Neurosci, 19 (18), pp. 7793-7803
  • Lozada, M., Romano, A., Maldonado, H., Long-term habituation to a danger stimulus in the crab Chasmagnathus granulatus (1990) Physiol Behav, 47 (1), pp. 35-41. , 10.1016/0031-9384(90)90039-7, 2326342
  • Tomsic, D., Romano, A., Maldonado, H., Behavioral and mechanistic bases of long-term habituation in the crab Chasmagnathus (1998) Adv Exp Med Biol, 446, pp. 17-35
  • Pedreira, M.E., Dimant, B., Maldonado, H., Inhibitors of protein and RNA synthesis block context memory and long-term habituation in the crab Chasmagnathus (1996) Pharmacol Biochem Behav, 54 (3), pp. 611-617. , 10.1016/0091-3057(95)02206-6, 8743637
  • Locatelli, F., LaFourcade, C., Maldonado, H., Romano, A., Characterisation of cAMP-dependent protein kinase isoforms in the brain of the crab Chasmagnathus (2001) J Comp Physiol [B], 171 (1), pp. 33-40
  • Locatelli, F., Maldonado, H., Romano, A., Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus (2002) Neurobiol Learn Mem, 77 (2), pp. 234-249. , 10.1006/nlme.2001.4007, 11848721
  • Feld, M., Dimant, B., Delorenzi, A., Coso, O., Romano, A., Phosphorylation of extra-nuclear ERK/MAPK is required for long-term memory consolidation in the crab Chasmagnathus (2005) Behav Brain Res, 158 (2), pp. 251-261. , 10.1016/j.bbr.2004.09.005, 15698891
  • Freudenthal, R., Romano, A., Participation of Rel/NF-kappaB transcription factors in long-term memory in the crab Chasmagnathus (2000) Brain Res, 855 (2), pp. 274-281. , 10.1016/S0006-8993(99)02358-6, 10677600
  • Merlo, E., Freudenthal, R., Romano, A., The IkappaB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus (2002) Neuroscience, 112 (1), pp. 161-172. , 10.1016/S0306-4522(02)00049-0, 12044481
  • Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T.J., Higgins, D.G., Thompson, J.D., Multiple sequence alignment with the Clustal series of programs (2003) Nucleic Acids Res, 31 (13), pp. 3497-3500. , 10.1093/nar/gkg500, 168907, 12824352
  • Luo, L., Tully, T., White, K., Human amyloid precursor protein ameliorates behavioral deficit of flies deleted for Appl gene (1992) Neuron, 9 (4), pp. 595-605. , 10.1016/0896-6273(92)90024-8, 1389179
  • Kimberly, W.T., Zheng, J.B., Guenette, S.Y., Selkoe, D.J., The intracellular domain of the beta-amyloid precursor protein is stabilized by Fe65 and translocates to the nucleus in a notch-like manner (2001) J Biol Chem, 276 (43), pp. 40288-40292
  • O'sullivan, N.C., McGettigan, P.A., Sheridan, G.K., Pickering, M., Conboy, L., O'Connor, J.J., Moynagh, P.N., Murphy, K.J., Temporal change in gene expression in the rat dentate gyrus following passive avoidance learning (2007) J Neurochem, 101 (4), pp. 1085-1098. , 10.1111/j.1471-4159.2006.04418.x, 17298388
  • Romano, A., Lozada, M., Maldonado, H., Effect of naloxone pretreatment on habituation in the crab Chasmagnathus granulatus (1990) Behav Neural Biol, 53 (1), pp. 113-122. , 10.1016/0163-1047(90)90882-7, 2154175
  • Chomczynski, P., Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction (1987) Anal Biochem, 162 (1), pp. 156-159. , 10.1016/0003-2697(87)90021-2, 2440339
  • Pfaffl, M.W., A new mathematical model for relative quantification in real-time RT-PCR (2001) Nucleic Acids Res, 29 (9), pp. e45. , 10.1093/nar/29.9.e45, 55695, 11328886
  • Sandeman, D., Sandeman, R., Derby, C., Schmidt, M., Morphology of the Brain of Crayfish, Crabs, and Spiny Lobsters: A Common Nomenclature for Homologous Structures (1992) Biol Bull, 183 (2), pp. 304-326

Citas:

---------- APA ----------
Fustiñana, M.S., Ariel, P., Federman, N., Freudenthal, R. & Romano, A. (2010) . Characterization of the beta amyloid precursor protein-like gene in the central nervous system of the crab Chasmagnathus. Expression during memory consolidation. BMC Neuroscience, 11.
http://dx.doi.org/10.1186/1471-2202-11-109
---------- CHICAGO ----------
Fustiñana, M.S., Ariel, P., Federman, N., Freudenthal, R., Romano, A. "Characterization of the beta amyloid precursor protein-like gene in the central nervous system of the crab Chasmagnathus. Expression during memory consolidation" . BMC Neuroscience 11 (2010).
http://dx.doi.org/10.1186/1471-2202-11-109
---------- MLA ----------
Fustiñana, M.S., Ariel, P., Federman, N., Freudenthal, R., Romano, A. "Characterization of the beta amyloid precursor protein-like gene in the central nervous system of the crab Chasmagnathus. Expression during memory consolidation" . BMC Neuroscience, vol. 11, 2010.
http://dx.doi.org/10.1186/1471-2202-11-109
---------- VANCOUVER ----------
Fustiñana, M.S., Ariel, P., Federman, N., Freudenthal, R., Romano, A. Characterization of the beta amyloid precursor protein-like gene in the central nervous system of the crab Chasmagnathus. Expression during memory consolidation. BMC Neurosci. 2010;11.
http://dx.doi.org/10.1186/1471-2202-11-109