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Abstract:

Pseudomonas sp. 14-3 accumulates polyhydroxybutyrate (PHB) from octanoate, but not from glucose. To elucidate this unusual phenotype, genes responsible for the synthesis of PHB were cloned and analyzed. A PHB polymerase gene (phaC) was found downstream from genes coding for a β-ketothiolase (phaA), an acetoacetyl-coenzyme A reductase (phaB) and a putative transcriptional regulator (phaR). All genes were similar to pha genes from several related species, but differences were observed in the distal region of phaA. Complementation with heterologous β-ketothiolase genes from Azotobacter sp. FA8 or Pseudomonas putida GPp104 restored the capability of Pseudomonas sp. 14-3 to synthesize PHB from glucose, demonstrating that its β-ketothiolase was nonfunctional. Analysis of the genome sequences of other Pseudomonas species has revealed the existence of putative β-ketothiolase genes. The functionality of one of these thiolase genes, belonging to P. putida GPp104, was experimentally demonstrated. Pseudomonas sp. 14-3 is the first natural phaA mutant described, that despite this mutation accumulates high amounts of PHB when growing on fatty acids. © 2006 Federation of European Microbiological Societies.

Registro:

Documento: Artículo
Título:Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene
Autor:Ayub, N.D.; Julia Pettinari, M.; Méndez, B.S.; López, N.I.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:β-ketothiolase; Natural mutants; Polyhydroxybutyrate; Pseudomonas; acetyl coenzyme A acyltransferase; butyric acid derivative; polyhydroxybutyrate; unclassified drug; article; Azotobacter; biosynthesis; controlled study; gene expression; gene mutation; gene sequence; genetic analysis; molecular cloning; nonhuman; nucleotide sequence; phenotype; priority journal; Pseudomonas; Pseudomonas putida; transcription regulation; Acetyl-CoA C-Acyltransferase; Bacterial Proteins; Base Sequence; Cloning, Molecular; Genetic Complementation Test; Genome, Bacterial; Glucose; Hydroxybutyrates; Molecular Sequence Data; Octanoic Acids; Polyesters; Pseudomonas; Sequence Alignment; Azotobacter; Pseudomonas; Pseudomonas putida; Pseudomonas sp. 14-3
Año:2006
Volumen:264
Número:1
Página de inicio:125
Página de fin:131
DOI: http://dx.doi.org/10.1111/j.1574-6968.2006.00446.x
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:acetyl coenzyme A acyltransferase, 9029-97-4; Acetyl-CoA C-Acyltransferase, 2.3.1.16; Bacterial Proteins; Glucose, 50-99-7; Hydroxybutyrates; Octanoic Acids; Polyesters; caprylic acid, 124-07-2; poly-beta-hydroxybutyrate, 26063-00-3
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03781097_v264_n1_p125_Ayub.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v264_n1_p125_Ayub

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Citas:

---------- APA ----------
Ayub, N.D., Julia Pettinari, M., Méndez, B.S. & López, N.I. (2006) . Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene. FEMS Microbiology Letters, 264(1), 125-131.
http://dx.doi.org/10.1111/j.1574-6968.2006.00446.x
---------- CHICAGO ----------
Ayub, N.D., Julia Pettinari, M., Méndez, B.S., López, N.I. "Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene" . FEMS Microbiology Letters 264, no. 1 (2006) : 125-131.
http://dx.doi.org/10.1111/j.1574-6968.2006.00446.x
---------- MLA ----------
Ayub, N.D., Julia Pettinari, M., Méndez, B.S., López, N.I. "Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene" . FEMS Microbiology Letters, vol. 264, no. 1, 2006, pp. 125-131.
http://dx.doi.org/10.1111/j.1574-6968.2006.00446.x
---------- VANCOUVER ----------
Ayub, N.D., Julia Pettinari, M., Méndez, B.S., López, N.I. Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene. FEMS Microbiol. Lett. 2006;264(1):125-131.
http://dx.doi.org/10.1111/j.1574-6968.2006.00446.x