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Abstract:

Somatolactin (SL) and SL receptor (SLR) belong to the growth hormone and cytokine type I receptor superfamilies, respectively. However, further research is required to define the duplications and functions of SL and its receptors in basal vertebrates including environmental background color adaptation in fish. In the present study, we cloned and sequenced SL and its putative receptor (SLR), classified and compared the sequences phylogenetically, and determined SL and SLR mRNA expression levels during long-term background color exposure in Cichlasoma dimerus, a freshwater South American cichlid. Our results show that C. dimerus SL and SLR share high sequence similarity with homologous from other perciform fish. Phylogenetic analysis indicates that C. dimerus SL belongs to the SLα clade sub-group. C. dimerus SLR is clearly a member of the GHR1 receptor subgroup, which includes the experimentally validated SLR from salmonids. Higher transcript levels of SLα in the pituitary and SLR in the epidermis and dermis cells of fish scales were observed in fish following long-term black background color exposure compared to those exposed to a white background. A higher number of melanophores was also observed in fish exposed for 10. days to a black background compared to those exposed to a white background. These changes were concomitant to differences in SL or SLR transcript levels found in fish exposed to these two different background colors. Our results suggest, for the first time, that SLR is expressed in fish scales, and that there is an increase in SL in the pituitary and the putative SLR in likely target cells, i.e., melanophores, in long-term black background exposure in C. dimerus. © 2011 Elsevier Inc.

Registro:

Documento: Artículo
Título:Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation
Autor:Cánepa, M.M.; Zhu, Y.; Fossati, M.; Stiller, J.W.; Vissio, P.G.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
CONICET, Argentina
Department of Biology, East Carolina University, Greenville, NC 27858, United States
School of Biological Sciences, Flinders University, G.P.O. Box 2100, Adelaide, SA 5001, Australia
Palabras clave:Background color exposure; Chromatophore; Cichlid; Melanophore; Pituitary; Somatolactin; Somatolactin receptor; Teleost; growth hormone receptor; messenger RNA; somatolectin; somatolectin receptor; unclassified drug; acclimatization; animal cell; animal experiment; article; Cichlasoma dimerus; cichlid; color; controlled study; epidermis cell; female; gene sequence; male; melanophore; molecular cloning; nonhuman; nucleotide sequence; Perciformes; phylogeny; priority journal; protein expression; salmonid; skin cell; species differentiation; unindexed sequence
Año:2012
Volumen:176
Número:1
Página de inicio:52
Página de fin:61
DOI: http://dx.doi.org/10.1016/j.ygcen.2011.12.023
Título revista:General and Comparative Endocrinology
Título revista abreviado:Gen. Comp. Endocrinol.
ISSN:00166480
CODEN:GCENA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v176_n1_p52_Canepa

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Citas:

---------- APA ----------
Cánepa, M.M., Zhu, Y., Fossati, M., Stiller, J.W. & Vissio, P.G. (2012) . Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation. General and Comparative Endocrinology, 176(1), 52-61.
http://dx.doi.org/10.1016/j.ygcen.2011.12.023
---------- CHICAGO ----------
Cánepa, M.M., Zhu, Y., Fossati, M., Stiller, J.W., Vissio, P.G. "Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation" . General and Comparative Endocrinology 176, no. 1 (2012) : 52-61.
http://dx.doi.org/10.1016/j.ygcen.2011.12.023
---------- MLA ----------
Cánepa, M.M., Zhu, Y., Fossati, M., Stiller, J.W., Vissio, P.G. "Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation" . General and Comparative Endocrinology, vol. 176, no. 1, 2012, pp. 52-61.
http://dx.doi.org/10.1016/j.ygcen.2011.12.023
---------- VANCOUVER ----------
Cánepa, M.M., Zhu, Y., Fossati, M., Stiller, J.W., Vissio, P.G. Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation. Gen. Comp. Endocrinol. 2012;176(1):52-61.
http://dx.doi.org/10.1016/j.ygcen.2011.12.023