Abstract:
In this work we confirm by a metabolic method the existence of at least two enzymes with 11β- and 18-hydroxylase activities in rat adrenal mitochondria. The method was based on the ability of cortisol (F), a foreign alternative substrate, to inhibit competitively metabolite productions from various precursors. F inhibited a) aldosterone (ALDO) production from 11-deoxycorticosterone (DOC) without affecting the yields of corticosterone (B) and 18-hydroxy-11-deoxycorticosterone (18-OHDOC); b) 18-hydroxycorticosterone and aldosterone productions from B (Ki = 2.5 ± 0.5 μM); and c) ALDO production from 18-OHDOC. These results suggest the existence of two categories of enzymes with both 11β- and 18-hydroxylase activities, one comprising those that catalyze the conversions of DOC to B and 18-OHDOC (F-insensitive reactions [FIS]) and the other one comprising the enzymes involved in the conversions of B to 18-OHB and ALDO and that of 18-OHDOC to ALDO (F-sensitive reactions [FS]). The cloned enzymes CYP11B1 and CYP11B2 would pertain respectively to the FIS and FS categories. © 1995.
Registro:
Documento: |
Artículo
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Título: | Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria |
Autor: | Matkovic, L.; Gomez-Sanchez, C.E.; Lantos, C.P.; Cozza, E.N. |
Filiación: | Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina Department of Internal Medicine, Research Service, Harry S Truman Memorial Veterans' Hospital, Columbia, MO, United States
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Palabras clave: | 11β, 18-hydroxylases; adrenal; aldosterone; Cortisol; cytochrome P450; steroidogenesis; 18 hydroxycorticosterone; 18 hydroxydeoxycorticosterone; aldosterone; cytochrome p450; deoxycorticosterone; hydrocortisone; mitochondrial enzyme; oxygenase; steroid 11beta monooxygenase; aldosterone release; animal tissue; article; enzyme activity; enzyme localization; enzyme substrate; male; mitochondrion; molecular cloning; nonhuman; rat; steroidogenesis; zona glomerulosa; 18-Hydroxycorticosterone; Adrenal Glands; Aldosterone; Aldosterone Antagonists; Animal; Desoxycorticosterone; Hydrocortisone; Male; Mitochondria; Mixed Function Oxygenases; Rats; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Animalia |
Año: | 1995
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Volumen: | 60
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Número: | 6
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Página de inicio: | 447
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Página de fin: | 452
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DOI: |
http://dx.doi.org/10.1016/0039-128X(94)00064-J |
Título revista: | Steroids
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Título revista abreviado: | Steroids
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ISSN: | 0039128X
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CODEN: | STEDA
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CAS: | 18-Hydroxycorticosterone, 561-65-9; 18-hydroxydeoxycorticosterone, 379-68-0; Aldosterone Antagonists; Aldosterone, 52-39-1; Desoxycorticosterone, 64-85-7; Hydrocortisone, 50-23-7; Mixed Function Oxygenases, EC 1.-
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v60_n6_p447_Matkovic |
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Citas:
---------- APA ----------
Matkovic, L., Gomez-Sanchez, C.E., Lantos, C.P. & Cozza, E.N.
(1995)
. Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria. Steroids, 60(6), 447-452.
http://dx.doi.org/10.1016/0039-128X(94)00064-J---------- CHICAGO ----------
Matkovic, L., Gomez-Sanchez, C.E., Lantos, C.P., Cozza, E.N.
"Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria"
. Steroids 60, no. 6
(1995) : 447-452.
http://dx.doi.org/10.1016/0039-128X(94)00064-J---------- MLA ----------
Matkovic, L., Gomez-Sanchez, C.E., Lantos, C.P., Cozza, E.N.
"Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria"
. Steroids, vol. 60, no. 6, 1995, pp. 447-452.
http://dx.doi.org/10.1016/0039-128X(94)00064-J---------- VANCOUVER ----------
Matkovic, L., Gomez-Sanchez, C.E., Lantos, C.P., Cozza, E.N. Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria. Steroids. 1995;60(6):447-452.
http://dx.doi.org/10.1016/0039-128X(94)00064-J