Argimón, S.; Galello, F.; Pereyra, E.; Rossi, S.; Moreno, S. "Mucor rouxii Rho1 protein; characterization and possible role in polarized growth" (2007) Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 91(3):237-251
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We have previously shown that protein kinase A of the medically important zygomycete Mucor rouxii participates in fungal morphology through cytoskeletal organization. As a first step towards finding the link between protein kinase A and cytoskeletal organization we here demonstrate the cloning of the Rho1 gene and the characterization of its protein product. The RHO1 protein primary sequence shows 70-85% identity with fungal RHO1 or mammalian RhoA. Two protein kinase A phosphorylation sequences in adequate context are predicted, Ser73 and Ser135. The peptide IRRNSQKFV, containing Ser135 proved to be a good substrate for M. rouxii protein kinase A catalytic subunit. The over-expressed Rho1 fully complements a Saccharomyces cerevisiae null mutant. The endogenous protein was identified by western blot against a developed antibody and by ADP-ribosylation. Localization in germlings was visualized by immunofluorescence; the protein was localized in patches in the mother cell surface and excluded from the germ tube. Measurement of Rho1 expression during germination indicates that Rho1, at both the mRNA and protein levels, correlates with differentiation and not with growth. Rho1 has been shown to be the regulatory protein of the β-1,3-glucan synthase complex in fungi in which β-1,3-glucans are major components of the cell wall. Even though glucans have not been detected in zygomycetes, caspofungin, an echinochandin known to be an inhibitor of β-1,3-glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. This result has an important impact on the possible participation of β-1,3-glucans on the regulation of morphology of zygomycetes. © Springer Science+Business Media B.V. 2006.


Documento: Artículo
Título:Mucor rouxii Rho1 protein; characterization and possible role in polarized growth
Autor:Argimón, S.; Galello, F.; Pereyra, E.; Rossi, S.; Moreno, S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina
Aberdeen Fungal Group, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom
Palabras clave:Caspofungin; Cloning; Gene expression morphology; Mucor rouxii; Rho1; 1,3 beta glucan synthase; beta 1,3 glucan; caspofungin; cyclic AMP dependent protein kinase; fungal protein; messenger RNA; protein Rho1; RhoA guanine nucleotide binding protein; serine; unclassified drug; adenosine diphosphate ribosylation; amino acid sequence; antifungal activity; article; cell differentiation; controlled study; correlation function; culture medium; enzyme active site; enzyme phosphorylation; enzyme substrate; fungal cell; fungal gene; fungal morphology; fungus culture; fungus growth; fungus mutant; gene overexpression; immunofluorescence test; molecular cloning; Mucor; mucor rouxii; nonhuman; nucleotide sequence; prediction; priority journal; protein analysis; protein function; protein localization; Saccharomyces cerevisiae; sequence homology; Western blotting; Amino Acid Sequence; Base Sequence; beta-Glucans; Cloning, Molecular; Fungal Proteins; Gene Expression Regulation, Fungal; Molecular Sequence Data; Mucor; Phylogeny; Amylomyces rouxii; Fungi; Mammalia; Saccharomyces cerevisiae; Zygomycetes
Página de inicio:237
Página de fin:251
Título revista:Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Título revista abreviado:Antonie Van Leeuwenhoek Int. J. Gen. Mol. Microbiol.
CAS:1,3 beta glucan synthase, 9037-30-3; beta 1,3 glucan, 9051-97-2; caspofungin, 189768-38-5; serine, 56-45-1, 6898-95-9; Fungal Proteins; beta-1,3-glucan, 9051-97-2; beta-Glucans


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---------- APA ----------
Argimón, S., Galello, F., Pereyra, E., Rossi, S. & Moreno, S. (2007) . Mucor rouxii Rho1 protein; characterization and possible role in polarized growth. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 91(3), 237-251.
---------- CHICAGO ----------
Argimón, S., Galello, F., Pereyra, E., Rossi, S., Moreno, S. "Mucor rouxii Rho1 protein; characterization and possible role in polarized growth" . Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology 91, no. 3 (2007) : 237-251.
---------- MLA ----------
Argimón, S., Galello, F., Pereyra, E., Rossi, S., Moreno, S. "Mucor rouxii Rho1 protein; characterization and possible role in polarized growth" . Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, vol. 91, no. 3, 2007, pp. 237-251.
---------- VANCOUVER ----------
Argimón, S., Galello, F., Pereyra, E., Rossi, S., Moreno, S. Mucor rouxii Rho1 protein; characterization and possible role in polarized growth. Antonie Van Leeuwenhoek Int. J. Gen. Mol. Microbiol. 2007;91(3):237-251.