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Abstract:

Calcium-dependent protein kinases (CDPKs) are essential calcium sensors. In this work, we have studied StCDPK2 isoform from potato both at gene and protein level. StCdpk2 genomic sequence contains eight exons and seven introns, as was observed for StCdpk1. There is one copy of the gene per genome located in chromosome 7. StCDPK2 encodes an active CDPK of 515 aminoacids, with an apparent MW of 57 kDa, which presents myristoylation and palmitoylation consensus in its N-terminus. StCDPK2 is highly expressed in leaves and green sprouts; enhanced expression was detected under light treatment, which corresponds well with light responsive cis-acting elements found in its promoter sequence. Antibodies against the recombinant StCDPK2::6xHis protein detected this isoform in soluble and particulate fractions from leaves. StCDPK2 autophosphorylation and kinase activity are both calcium dependent reaching half maximal activation at 0.6 μM calcium. The active kinase is autophosphorylated on serine and tyrosine residues and its activity is negatively modulated by phosphatidic acid (PA). Our results reveal StCDPK2 as a signalling element involved in plant growth and development and show that its activity is tightly regulated. © 2010 Springer-Verlag.

Registro:

Documento: Artículo
Título:StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling
Autor:Giammaria, V.; Grandellis, C.; Bachmann, S.; Gargantini, P.R.; Feingold, S.E.; Bryan, G.; Ulloa, R.M.
Filiación:Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI), CONICET and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490 2do piso, 1428 Buenos Aires, Argentina
Laboratorio de Agrobiotecnología, EEA Balcarce-INTA, C.C. 276 (7620) Balcarce, Argentina
Genetics Programme, Scottish Crop Research Institute (SCRI), Dundee DD2 5DA, United Kingdom
Facultad de Medicina, Universidad Católica de Córdoba (UCC), Jacinto Ríos 571, 5000 Córdoba, Argentina
Palabras clave:Autophosphorylation; CDPK; Light; Phospholipids; Solanum tuberosum; calcium; calcium binding protein; calcium protein kinase; calcium-dependent protein kinase; isoprotein; protein kinase; recombinant protein; vegetable protein; amino acid sequence; article; chemistry; enzymology; gene expression regulation; genetics; growth, development and aging; light; metabolism; molecular cloning; molecular genetics; nucleotide sequence; phosphorylation; potato; promoter region; sequence homology; signal transduction; upregulation; Amino Acid Sequence; Calcium; Calcium-Binding Proteins; Cloning, Molecular; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Light; Molecular Sequence Data; Phosphorylation; Plant Proteins; Promoter Regions, Genetic; Protein Isoforms; Protein Kinases; Recombinant Proteins; Sequence Homology, Amino Acid; Signal Transduction; Solanum tuberosum; Up-Regulation; Solanum tuberosum
Año:2011
Volumen:233
Número:3
Página de inicio:593
Página de fin:609
DOI: http://dx.doi.org/10.1007/s00425-010-1319-2
Título revista:Planta
Título revista abreviado:Planta
ISSN:00320935
CODEN:PLANA
CAS:calcium, 14092-94-5, 7440-70-2; protein kinase, 9026-43-1; Calcium, 7440-70-2; Calcium-Binding Proteins; Plant Proteins; Protein Isoforms; Protein Kinases, 2.7.-; Recombinant Proteins; calcium-dependent protein kinase, 2.7.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v233_n3_p593_Giammaria

Referencias:

  • Andronis, C., Barak, S., Knowles, S.M., Sugano, S., Tobin, E.M., The clock protein CCA1 and the bZIP transcription factor HY5 physically interact to regulate gene expression in Arabidopsis (2008) Mol Plant, 1, pp. 58-67
  • Arguello-Astorga, G., Herrera-Estrella, L., Evolution of light-regulated plant promoters (1998) Annu Rev Plant Physiol Plant Mol Biol, 49, pp. 525-555
  • Asano, T., Tanaka, N., Yang, G., Hayashi, N., Komatsu, S., Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rice (2005) Plant Cell Physiol, 46, pp. 356-366
  • Bachmann, M., Shiraishi, N., Campbell, W.H., Yoo, B.C., Harmon, A.C., Huber, S.C., Identification of the major regulatory phosphorylation site as Ser-543 in spinach leaf nitrate reductase and its phosphorylation by a calcium-dependent protein kinase in vitro (1996) Plant Cell, 8, pp. 505-517
  • Bonierbale, M.W., Plaisted, R.L., Pineda, O., Tanksley, S.D., QTL analysis of trichome-mediated insect resistance in potato (1994) Theor Appl Genet, 87, pp. 973-987
  • Chaudhuri, S., Seal, A., Gupta, M.D., Autophosphorylation-dependent activation of a calcium-dependent protein kinase from groundnut (1999) Plant Physiol, 120, pp. 859-866
  • Chen, X., Lin, W.H., Wang, Y., Luan, S., Xue, H.W., An inositol polyphosphate 5-phosphatase functions in PHOTOTROPIN1 signaling in Arabidopis by altering cytosolic Ca2+ (2008) Plant Cell, 20, pp. 353-366
  • Cheng, S.-H., Willmann, M.R., Chen, H.-C., Sheen, J., Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family (2002) Plant Physiol, 129, pp. 469-485
  • Chico, J.M., Raíces, M., Téllez-Iñón, M.T., Ulloa, R.M., A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants (2002) Plant Physiol, 128, pp. 256-270
  • Choi, H.I., Park, H.J., Park, J.H., Kim, S., Im, M.Y., Seo, H.H., Kim, Y.W., Kim, S.Y., Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity (2005) Plant Physiol, 139, pp. 1750-1761
  • Dyson, H.J., Wright, P.E., Elucidation of the protein folding landscape by NMR (2005) Methods Enzymol, 394, pp. 299-321
  • Ellard-Ivey, M., Hopkins, R.B., White, T.J., Lomax, T.L., Cloning, expression and N-terminal myristoylation of CpCPK1, a calcium-dependent protein kinase from zucchini (Cucurbita pepo L.) (1999) Plant Mol Biol, 39, pp. 199-208
  • Falquet, L., Pagni, M., Bucher, P., Hulo, N., Sigrist, C.J., Hofmann, K., Bairoch, A., The PROSITE database, its status in 2002 (2002) Nucleic Acids Res, 30, pp. 235-238
  • Farmer, P., Choi, J., Calcium and phospholipid activation of a recombinant calcium-dependent protein kinase (DcCPK1) from carrot (Daucus carota L.) (1999) Biochim Biophys Acta, 14, pp. 6-17
  • Feingold, S., Lloyd, J., Norero, N., Bonierbale, M., Lorenzen, J., Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.) (2005) Theor Appl Genet, 111, pp. 456-466
  • Gargantini, P.R., Gonzalez-Rizzo, S., Chinchilla, D., Raices, M., Giammaria, V., Ulloa, R.M., Frugier, F., Crespi, M.D., A CDPK isoform participates in the regulation of nodule number in Medicago truncatula (2006) Plant J, 48, pp. 843-856
  • Gargantini, P.R., Giammaria, V., Grandellis, C., Feingold, S.E., Maldonado, S., Ulloa, R.M., Genomic and functional characterization of StCDPK1 (2009) Plant Mol Biol, 70, pp. 153-172
  • Gehring, C.A., Williams, D.A., Cody, S.H., Parish, R.W., Phototropism and geotropism in maize coleoptiles are spatially correlated with increases in cytosolic free calcium (1990) Nature, 345, pp. 528-530
  • Gidoni, D., Brosio, P., Bond-Nutter, D., Bedbrook, J., Dunsmuir, P., Novel cis-acting elements in Petunia Cab gene promoters (1989) Mol Gen Genet, 215, pp. 337-344
  • Harada, A., Shimazaki, K., Phototropins and blue light-dependent calcium signaling in higher plants (2007) Photochem Photobiol, 83, pp. 102-111
  • Harmon, A.C., Calcium-regulated protein kinases of plants (2003) Gravit Space Biol Bull, 16, pp. 83-90
  • Harmon, A.C., Gribskov, M., Harper, J.F., CDPKs-a kinase for every Ca2+ signal? (2000) Trends Plant Sci, 5, pp. 154-159
  • Harper, J.F., Binder, B.M., Sussman, M.R., Calcium and lipid regulation of an Arabidopsis protein kinase expressed in Escherichia coli (1993) Biochemistry, 32, pp. 3282-3290
  • Harper, J.F., Breton, G., Harmon, A., Decoding Ca2+ signals through plant protein kinases (2004) Annu Rev Plant Biol, 55, pp. 263-288
  • Harris, N., Spence, J., Oparka, K.J., Immunocytochemistry for light and electron microscopy (1994) Plant Cell Biology: A Practical Approach, pp. 157-176. , N. Harris and K. J. Oparka (Eds.), Oxford: IRL Press at Oxford University Press
  • Hein, I., McLean, K., Chalhoub, B., Bryan, G., Generation and screening of a BAC library from a diploid potato clone to unravel durable late blight resistance on linkage group IV (2007) Int J Plant Genomics, , (article ID 51421)
  • Hrabak, E.M., Chan, C.W., Gribskov, M., Harper, J.F., Choi, J.H., Halford, N., Kudla, J., Harmon, A.C., The Arabidopsis CDPK-SnRK superfamily of protein kinases (2003) Plant Physiol, 132, pp. 666-680
  • Ishida, S., Yuasa, T., Nakata, M., Takahashi, Y., A tobacco calcium-dependent protein kinase, CDPK1, regulates the transcription factor REPRESSION OF SHOOT GROWTH in response to gibberellins (2008) Plant Cell, 20, pp. 3273-3288
  • Ito, T., Nakata, M., Fukazawa, J., Ishida, S., Takahashi, Y., Alteration of substrate specificity: the variable N-terminal domain of tobacco Ca2+-dependent protein kinase is important for substrate recognition (2010) Plant Cell, 22, pp. 1592-1604
  • Johnson, C.H., Knight, M.R., Kondo, T., Masson, P., Sedbrook, J., Haley, A., Trewavas, A., Circadian oscillations of cytosolic and chloroplastic free calcium in plants (1995) Science, 269, pp. 1863-1865
  • Kim, M.C., Chung, W.S., Yun, D.-J., Cho, M.J., Calcium and calmodulin-mediated regulation of gene expression in plants (2009) Mol Plant, 2, pp. 13-21
  • Klimecka, M., Muszyńska, G., Structure and functions of plant calcium-dependent protein kinases (2007) Acta Biochim Pol, 54, pp. 219-233
  • Kobayashi, M., Ohura, I., Kawakita, K., Yokota, N., Fujiwara, M., Shimamoto, K., Doke, N., Yoshioka, H., Calcium-dependent protein kinases regulate the production of reactive oxygen species by potato NADPH oxidase (2007) Plant Cell, 19, pp. 1065-1080
  • Kooijman, E.E., Tieleman, D.P., Testerink, C., Munnik, T., Rijkers, D.T., Burger, K.N., de Kruijff, B., An electrostatic/hydrogen bond switch as the basis for the specific interaction of phosphatidic acid with proteins (2007) J Biol Chem, 282, pp. 11356-11364
  • Kooijman, E.E., King, K.E., Gangoda, M., Gericke, A., Ionization properties phosphatidylinositol polyphosphates in mixed model membranes (2009) Biochemistry, 48, pp. 9360-9371
  • Li, A., Zhu, Y.F., Tan, X.M., Wang, X., Wei, B., Guo, H.Z., Zhang, Z.L., Mao, L., Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.) (2008) Plant Mol Biol, 66, pp. 429-443
  • Li, A., Wang, X., Leseberg, C.H., Jia, J., Mao, L., Biotic and abiotic stress responses through calcium-dependent protein kinase (CDPK) signaling in wheat (Triticum aestivum L.) (2008) Plant Signal Behav, 3, pp. 654-656
  • Lu, S., Hrabak, E., An Arabidopsis calcium-dependent protein kinase is associated with the endoplasmic reticulum (2002) Plant Physiol, 12, pp. 1008-1021
  • Ludwig, A., Romeis, T., Jones, J., CDPK-mediated signalling pathways: specificity and cross-talk (2004) J Exp Bot, 55, pp. 181-188
  • Martín, M.L., Busconi, L., Membrane localization of a rice calcium-dependent protein kinase (CDPK) is mediated by myristoylation and palmitoylation (2000) Plant J, 24, pp. 429-435
  • Monreal, J.A., López-Baena, F.J., Vidal, J., Echevarría, C., García-Mauriño, S., Involvement of phospholipase D and phosphatidic acid in the light-dependent up-regulation of sorghum leaf phosphoenolpyruvate carboxylase-kinase (2010) J Exp Bot, 61, pp. 2819-2827
  • Munnik, T., Testerink, C., Plant phospholipid signaling: "in a nutshell" (2009) J Lipid Res, 50 (SUPPL), pp. S260-S265
  • Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures (1962) Plant Physiol, 15, pp. 473-497
  • Murray, M.G., Thompson, W.F., Rapid isolation of high molecular weight plant DNA (1980) Nucleic Acids Res, 8, pp. 4321-4325
  • Raices, M., Gargantini, P.R., Chinchilla, D., Crespi, M., Tellez-Inon, M.T., Ulloa, R.M., Regulation of CDPK isoforms during tuber development (2003) Plant Mol Biol, 52, pp. 1011-1024
  • Raíces, M., Chico, J.M., Téllez-Iñón, M.T., Ulloa, R.M., Molecular characterization of StCDPK1, a calcium-dependent protein kinase from Solanum tuberosum that is induced at the onset of tuber development (2001) Plant Mol Biol, 46, pp. 591-601
  • Romeis, T., Piedras, P., Jones, J.D.G., Resistance gene dependent activation of a calcium-dependent protein kinase in the plant defense response (2000) Plant Cell, 12, pp. 803-815
  • Romeis, T., Ludwig, A.A., Martin, R., Jones, J.D., Calcium-dependent protein kinases play an essential role in a plant defence response (2001) EMBO J, 20, pp. 5556-5567
  • Saha, P., Singh, M., Characterization of a winged bean (Psophocarpus tetragonolobus) protein kinase with calmodulin-like domain: regulation by autophosphorylation (1995) Biochem J, 305, pp. 205-210
  • Schaller, G.E., Harmon, A.C., Sussman, M.R., Characterization of a calcium- and lipid-dependent protein kinase associated with the plasma membrane of oat (1992) Biochemistry, 31, pp. 1721-1727
  • Schindler, U., Cashmore, A.R., Photoregulated gene expression may involve ubiquitous DNA binding proteins (1990) EMBO J, 9, pp. 3415-3427
  • Schlessinger, A., Liu, J., Rost, B., Natively unstructured loops differ from other loops (2007) PLoS Comput Biol, 3, pp. e140
  • Shapiro, M.B., Senapathy, P., RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression (1987) Nucleic Acids Res, 15, pp. 7155-7714
  • Sutoh, K., Yamauchi, D., Two cis-acting elements necessary and sufficient for gibberellin-upregulated proteinase expression in rice seeds (2003) Plant J, 34, pp. 635-645
  • Szczegielniak, J., Liwosz, A., Jurkowski, I., Loog, M., Dobrowolska, G., Ek, P., Harmon, A.C., Muszyńska, G., Calcium-dependent protein kinase from maize seedlings activated by phospholipids (2000) Eur J Biochem, 267, pp. 3818-3827
  • Szczegielniak, J., Klimecka, M., Liwosz, A., Ciesielski, A., Kaczanowski, S., Dobrowolska, G., Harmon, A.C., Muszyńska, G., A wound-responsive and phospholipid-regulated maize calcium-dependent protein kinase (2005) Plant Physiol, 139, pp. 1970-1983
  • Ulloa, R.M., Raíces, M., Macintosh, G.C., Maldonado, S., Téllez-Iñón, M.T., Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in Solanum tuberosum (2002) Physiol Plant, 115, pp. 417-427
  • van Os, H., Andrzejewski, S., Bakker, E., Construction of a 10, 000-marker ultra-dense genetic recombination map of potato: providing a framework for accelerated gene isolation and a genome wide physical map (2006) Genetics, 173, pp. 1075-1087
  • van Schooten, B., Testerink, C., Munnik, T., Signalling diacylglycerol pyrophosphate, a new phosphatidic acid metabolite (2006) Biochim Biophys Acta, 1761, pp. 151-159
  • Witte, C.P., Keinath, N., Dubiella, U., Demoulière, R., Seal, A., Romeis, T., Tobacco calcium-dependent protein kinases are differentially phosphorylated in vivo as part of a kinase cascade that regulates stress response (2010) J Biol Chem, 285, pp. 9740-9748

Citas:

---------- APA ----------
Giammaria, V., Grandellis, C., Bachmann, S., Gargantini, P.R., Feingold, S.E., Bryan, G. & Ulloa, R.M. (2011) . StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling. Planta, 233(3), 593-609.
http://dx.doi.org/10.1007/s00425-010-1319-2
---------- CHICAGO ----------
Giammaria, V., Grandellis, C., Bachmann, S., Gargantini, P.R., Feingold, S.E., Bryan, G., et al. "StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling" . Planta 233, no. 3 (2011) : 593-609.
http://dx.doi.org/10.1007/s00425-010-1319-2
---------- MLA ----------
Giammaria, V., Grandellis, C., Bachmann, S., Gargantini, P.R., Feingold, S.E., Bryan, G., et al. "StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling" . Planta, vol. 233, no. 3, 2011, pp. 593-609.
http://dx.doi.org/10.1007/s00425-010-1319-2
---------- VANCOUVER ----------
Giammaria, V., Grandellis, C., Bachmann, S., Gargantini, P.R., Feingold, S.E., Bryan, G., et al. StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling. Planta. 2011;233(3):593-609.
http://dx.doi.org/10.1007/s00425-010-1319-2