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Calcium-dependent protein kinases (CDPKs) are essential calcium sensors. In this work, we have studied StCDPK2 isoform from potato both at gene and protein level. StCdpk2 genomic sequence contains eight exons and seven introns, as was observed for StCdpk1. There is one copy of the gene per genome located in chromosome 7. StCDPK2 encodes an active CDPK of 515 aminoacids, with an apparent MW of 57 kDa, which presents myristoylation and palmitoylation consensus in its N-terminus. StCDPK2 is highly expressed in leaves and green sprouts; enhanced expression was detected under light treatment, which corresponds well with light responsive cis-acting elements found in its promoter sequence. Antibodies against the recombinant StCDPK2::6xHis protein detected this isoform in soluble and particulate fractions from leaves. StCDPK2 autophosphorylation and kinase activity are both calcium dependent reaching half maximal activation at 0.6 μM calcium. The active kinase is autophosphorylated on serine and tyrosine residues and its activity is negatively modulated by phosphatidic acid (PA). Our results reveal StCDPK2 as a signalling element involved in plant growth and development and show that its activity is tightly regulated. © 2010 Springer-Verlag.


Documento: Artículo
Título:StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling
Autor:Giammaria, V.; Grandellis, C.; Bachmann, S.; Gargantini, P.R.; Feingold, S.E.; Bryan, G.; Ulloa, R.M.
Filiación:Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI), CONICET and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490 2do piso, 1428 Buenos Aires, Argentina
Laboratorio de Agrobiotecnología, EEA Balcarce-INTA, C.C. 276 (7620) Balcarce, Argentina
Genetics Programme, Scottish Crop Research Institute (SCRI), Dundee DD2 5DA, United Kingdom
Facultad de Medicina, Universidad Católica de Córdoba (UCC), Jacinto Ríos 571, 5000 Córdoba, Argentina
Palabras clave:Autophosphorylation; CDPK; Light; Phospholipids; Solanum tuberosum; calcium; calcium binding protein; calcium protein kinase; calcium-dependent protein kinase; isoprotein; protein kinase; recombinant protein; vegetable protein; amino acid sequence; article; chemistry; enzymology; gene expression regulation; genetics; growth, development and aging; light; metabolism; molecular cloning; molecular genetics; nucleotide sequence; phosphorylation; potato; promoter region; sequence homology; signal transduction; upregulation; Amino Acid Sequence; Calcium; Calcium-Binding Proteins; Cloning, Molecular; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Light; Molecular Sequence Data; Phosphorylation; Plant Proteins; Promoter Regions, Genetic; Protein Isoforms; Protein Kinases; Recombinant Proteins; Sequence Homology, Amino Acid; Signal Transduction; Solanum tuberosum; Up-Regulation; Solanum tuberosum
Página de inicio:593
Página de fin:609
Título revista:Planta
Título revista abreviado:Planta
CAS:calcium, 14092-94-5, 7440-70-2; protein kinase, 9026-43-1; Calcium, 7440-70-2; Calcium-Binding Proteins; Plant Proteins; Protein Isoforms; Protein Kinases, 2.7.-; Recombinant Proteins; calcium-dependent protein kinase, 2.7.1.-


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---------- APA ----------
Giammaria, V., Grandellis, C., Bachmann, S., Gargantini, P.R., Feingold, S.E., Bryan, G. & Ulloa, R.M. (2011) . StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling. Planta, 233(3), 593-609.
---------- CHICAGO ----------
Giammaria, V., Grandellis, C., Bachmann, S., Gargantini, P.R., Feingold, S.E., Bryan, G., et al. "StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling" . Planta 233, no. 3 (2011) : 593-609.
---------- MLA ----------
Giammaria, V., Grandellis, C., Bachmann, S., Gargantini, P.R., Feingold, S.E., Bryan, G., et al. "StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling" . Planta, vol. 233, no. 3, 2011, pp. 593-609.
---------- VANCOUVER ----------
Giammaria, V., Grandellis, C., Bachmann, S., Gargantini, P.R., Feingold, S.E., Bryan, G., et al. StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling. Planta. 2011;233(3):593-609.