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Abstract:

We characterized a persistently Junin virus (JUNV)-infected BHK-21 cell line obtained by experimental infection with the XJC13 strain. This cell line, named K3, produced low levels of virus in supernatants which were not influenced by the presence of defective interfering (DI) particles after the first year of infection. K3 cells were able to exclude superinfection of the homologous JUNV and the antigenically related Tacaribe virus (TCRV), whereas the non-related arenaviruses lymphocytic choriomeningitis virus (LCMV) and Pichinde virus (PICV) could replicate normally. Although superinfecting virus binding and internalization to persistently infected cells were slightly reduced, earlier biosynthesis of antigenomic RNA was observed in comparison with BHK-21 cells. Despite the fact that superinfection did not increase the number of cells expressing viral antigens, de novo synthesis of superinfecting virus proteins was detected. The virus produced by JUNV-superinfected K3 cells remained mostly cell-associated in the form of particles tethered to the plasma membrane and aberrant tubular structures. JUNV restriction was correlated with an overexpression of cellular protein TSG101 in K3 cells, which has been pointed out as involved in the budding of several RNA viruses. This correlation was also observed in a cell clone isolated from K3. Reduction of TSG101 expression favoured the release of infectious virus to the supernatant of JUNV-superinfected K3 cells. Our data suggest that overexpression of TSG101 in K3 cells is a novel mechanism that may contribute, along with a diminished synthesis of superinfecting virus proteins, to explain superinfection exclusion in persistently arenavirus-infected cells. © 2007 SGM.

Registro:

Documento: Artículo
Título:Superinfection exclusion in BHK-21 cells persistently infected with Junín virus
Autor:Ellenberg, P.; Linero, F.N.; Scolaro, L.A.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Universidad de Buenos Aires, Pab. II, 4 Piso, Buenos Aires, Argentina
Palabras clave:cell protein; virus antigen; virus protein; virus RNA; animal cell; antigenicity; Arenavirus; article; biosynthesis; cell budding; cell count; cell isolation; cell membrane; cell strain BHK; controlled study; correlation analysis; gene overexpression; Junin virus; Lymphocytic choriomeningitis virus; molecular cloning; nonhuman; Pichinde arenavirus; priority journal; superinfection; supernatant; virus infection; virus particle; virus replication; virus strain; Animals; Antigens, Viral; Arenaviridae Infections; Cell Line; Cercopithecus aethiops; Cricetinae; Defective Viruses; DNA Primers; DNA, Complementary; Genome, Viral; Haplorhini; Junin virus; Kidney; Polymerase Chain Reaction; RNA, Viral; Superinfection; Vero Cells; Arenavirus; Junin virus; Lymphocytic choriomeningitis virus; Pichinde virus; RNA viruses; Tacaribe virus
Año:2007
Volumen:88
Número:10
Página de inicio:2730
Página de fin:2739
DOI: http://dx.doi.org/10.1099/vir.0.83041-0
Título revista:Journal of General Virology
Título revista abreviado:J. Gen. Virol.
ISSN:00221317
CODEN:JGVIA
CAS:Antigens, Viral; DNA Primers; DNA, Complementary; RNA, Viral
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v88_n10_p2730_Ellenberg

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Citas:

---------- APA ----------
Ellenberg, P., Linero, F.N. & Scolaro, L.A. (2007) . Superinfection exclusion in BHK-21 cells persistently infected with Junín virus. Journal of General Virology, 88(10), 2730-2739.
http://dx.doi.org/10.1099/vir.0.83041-0
---------- CHICAGO ----------
Ellenberg, P., Linero, F.N., Scolaro, L.A. "Superinfection exclusion in BHK-21 cells persistently infected with Junín virus" . Journal of General Virology 88, no. 10 (2007) : 2730-2739.
http://dx.doi.org/10.1099/vir.0.83041-0
---------- MLA ----------
Ellenberg, P., Linero, F.N., Scolaro, L.A. "Superinfection exclusion in BHK-21 cells persistently infected with Junín virus" . Journal of General Virology, vol. 88, no. 10, 2007, pp. 2730-2739.
http://dx.doi.org/10.1099/vir.0.83041-0
---------- VANCOUVER ----------
Ellenberg, P., Linero, F.N., Scolaro, L.A. Superinfection exclusion in BHK-21 cells persistently infected with Junín virus. J. Gen. Virol. 2007;88(10):2730-2739.
http://dx.doi.org/10.1099/vir.0.83041-0