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Abstract:

This study was aimed at determining the role of the crustacean hyperglycemic hormone (CHH) in the physiological compensation to both saline and thermal stress, in the freshwater crayfish Cherax quadricarinatus. By determining the expression of the CHH gene in the eyestalk of juvenile crayfish, we found that maximal induction of CHH was induced at high salinity (10 g/L) and low temperature (20 °C). In order to investigate the role of CHH in the physiological compensation to such stressful conditions, recombinant CHH was supplied to stressed animals. CHH-injected crayfish showed increased hemolymphatic levels of glucose, in accordance with a significant utilization of glycogen reserves from the hepatopancreas. Furthermore, CHH administration allowed stressed animals to regulate hemolymphatic sodium and potassium at more constant levels than controls. Taken together, these results suggest a relevant role of CHH in increasing the energy available intended for processes involved in the physiological compensation of C. quadricarinatus to both saline and thermal stress. © 2015, Springer-Verlag Berlin Heidelberg.

Registro:

Documento: Artículo
Título:Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress
Autor:Prymaczok, N.C.; Pasqualino, V.M.; Viau, V.E.; Rodríguez, E.M.; Medesani, D.A.
Filiación:Department of Biodiversity and Experimental Biology, FCEN, University of Buenos Aires, Buenos Aires, Argentina
Institute of Biodiversity, Experimental and Applied Biology (IBBEA), CONICET-UBA, Ciudad Universitaria, Pab. II, C1428EGA, Buenos Aires, Argentina
Palabras clave:Cherax quadricarinatus; CHH; Salinity; Stress; Temperature; arthropod protein; fresh water; glucose; glycogen; hormone; hyperglycemic hormone, crustacean; nerve protein; potassium; recombinant protein; sodium; amino acid sequence; animal; chemistry; cold; crayfish; drug effects; ecosystem; energy metabolism; genetics; hemolymph; hepatopancreas; metabolism; molecular cloning; molecular genetics; physiological stress; salinity; salt tolerance; signal transduction; Amino Acid Sequence; Animals; Arthropod Proteins; Astacoidea; Cloning, Molecular; Cold Temperature; Ecosystem; Energy Metabolism; Fresh Water; Glucose; Glycogen; Hemolymph; Hepatopancreas; Invertebrate Hormones; Molecular Sequence Data; Nerve Tissue Proteins; Potassium; Recombinant Proteins; Salinity; Salt-Tolerance; Signal Transduction; Sodium; Stress, Physiological
Año:2016
Volumen:186
Número:2
Página de inicio:181
Página de fin:191
DOI: http://dx.doi.org/10.1007/s00360-015-0954-0
Título revista:Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Título revista abreviado:J. Comp. Physiol. B Biochem. Syst. Environ. Physiol.
ISSN:01741578
CODEN:JCPBD
CAS:glucose, 50-99-7, 84778-64-3; glycogen, 9005-79-2; potassium, 7440-09-7; sodium, 7440-23-5; Arthropod Proteins; Glucose; Glycogen; hyperglycemic hormone, crustacean; Invertebrate Hormones; Nerve Tissue Proteins; Potassium; Recombinant Proteins; Sodium
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01741578_v186_n2_p181_Prymaczok

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Citas:

---------- APA ----------
Prymaczok, N.C., Pasqualino, V.M., Viau, V.E., Rodríguez, E.M. & Medesani, D.A. (2016) . Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress. Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 186(2), 181-191.
http://dx.doi.org/10.1007/s00360-015-0954-0
---------- CHICAGO ----------
Prymaczok, N.C., Pasqualino, V.M., Viau, V.E., Rodríguez, E.M., Medesani, D.A. "Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress" . Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology 186, no. 2 (2016) : 181-191.
http://dx.doi.org/10.1007/s00360-015-0954-0
---------- MLA ----------
Prymaczok, N.C., Pasqualino, V.M., Viau, V.E., Rodríguez, E.M., Medesani, D.A. "Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress" . Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, vol. 186, no. 2, 2016, pp. 181-191.
http://dx.doi.org/10.1007/s00360-015-0954-0
---------- VANCOUVER ----------
Prymaczok, N.C., Pasqualino, V.M., Viau, V.E., Rodríguez, E.M., Medesani, D.A. Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress. J. Comp. Physiol. B Biochem. Syst. Environ. Physiol. 2016;186(2):181-191.
http://dx.doi.org/10.1007/s00360-015-0954-0