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Pereira, C.A.; Alonso, G.D.; Paveto, M.C.; Iribarren, A.; Cabanas, M.L.; Torres, H.N.; Flawiá, M.M. "Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites" (2000) Journal of Biological Chemistry. 275(2):1495-1501
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Abstract:

This work contains the first description of a guanidino kinase in a flagellar unicellular parasite. The enzyme phosphorylates L-arginine and was characterized in preparations from Trypanosoma cruzi, the ethiological agent of Chagas' disease. The activity requires ATP and a divalent cation. Under Standard assay conditions (1 mM L-arginine), the presence of 5-fold higher concentrations of canavanine or histidine produced a greater than 50% enzyme inhibition. The base sequence of this enzyme revealed an open reading frame of 357 amino acids and a molecular weight of 40,201. The amino acid sequence shows all of the characteristic consensus blocks of the ATP:guanidino phosphotransferase family and a putative 'actinin-type' actin-binding domain. The highest amino acid identities of the T. cruzi sequence, about 70%, were with arginine kinases from Arthropoda. Southern and chromosome blots revealed that the kinase is encoded by a single-copy gene. Moreover, Northern blot analysis showed an mRNA subpopulation of about 2.0 kilobases, and Western blotting of T. cruzi-soluble polypeptides revealed a 40-kDa band. The finding in the parasite of a phosphagen and its biosynthetic pathway, which are totally different from those in mammalian host tissues, points out this arginine kinase as a possible chemotherapy target for Chagas' disease.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites
Autor:Pereira, C.A.; Alonso, G.D.; Paveto, M.C.; Iribarren, A.; Cabanas, M.L.; Torres, H.N.; Flawiá, M.M.
Filiación:Inst. Invest. Ing. Genet. y Biol. M., Consejo Nac. de Invest. Cie. y Tec., Universidad de Buenos Aires, Buenos Aires, Argentina
INGEBI, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:adenosine triphosphate; arginine; arginine kinase; canavanine; divalent cation; guanidine derivative; histidine; messenger RNA; phosphotransferase; amino acid sequence; article; Chagas disease; drug targeting; energy transfer; enzyme activity; enzyme analysis; enzyme inhibition; molecular cloning; molecular weight; nonhuman; nucleotide sequence; open reading frame; priority journal; protozoon; Trypanosoma cruzi; Amino Acid Sequence; Amino Acids; Animals; Arginine Kinase; Base Sequence; Cations, Divalent; Chromatography, Affinity; Chromosome Mapping; Cloning, Molecular; Genomic Library; Humans; Kinetics; Molecular Sequence Data; Phylogeny; Recombinant Proteins; Sequence Alignment; Sequence Homology, Amino Acid; Trypanosoma cruzi; Arthropoda; Mammalia; Protozoa; Trypanosoma; Trypanosoma cruzi
Año:2000
Volumen:275
Número:2
Página de inicio:1495
Página de fin:1501
DOI: http://dx.doi.org/10.1074/jbc.275.2.1495
Título revista:Journal of Biological Chemistry
Título revista abreviado:J. Biol. Chem.
ISSN:00219258
CODEN:JBCHA
CAS:Amino Acids; Arginine Kinase, EC 2.7.3.3; Cations, Divalent; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v275_n2_p1495_Pereira

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Citas:

---------- APA ----------
Pereira, C.A., Alonso, G.D., Paveto, M.C., Iribarren, A., Cabanas, M.L., Torres, H.N. & Flawiá, M.M. (2000) . Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites. Journal of Biological Chemistry, 275(2), 1495-1501.
http://dx.doi.org/10.1074/jbc.275.2.1495
---------- CHICAGO ----------
Pereira, C.A., Alonso, G.D., Paveto, M.C., Iribarren, A., Cabanas, M.L., Torres, H.N., et al. "Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites" . Journal of Biological Chemistry 275, no. 2 (2000) : 1495-1501.
http://dx.doi.org/10.1074/jbc.275.2.1495
---------- MLA ----------
Pereira, C.A., Alonso, G.D., Paveto, M.C., Iribarren, A., Cabanas, M.L., Torres, H.N., et al. "Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites" . Journal of Biological Chemistry, vol. 275, no. 2, 2000, pp. 1495-1501.
http://dx.doi.org/10.1074/jbc.275.2.1495
---------- VANCOUVER ----------
Pereira, C.A., Alonso, G.D., Paveto, M.C., Iribarren, A., Cabanas, M.L., Torres, H.N., et al. Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites. J. Biol. Chem. 2000;275(2):1495-1501.
http://dx.doi.org/10.1074/jbc.275.2.1495