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Abstract:

The promoter region of two sunflower (Helianthus annuus L. HA89 genotype) seed specifically expressed genes, coding for an oleate desaturase (HaFAD2-1) and a lipid transfer protein (HaAP10), were cloned and in silico characterized. The isolated fragments are 867 and 964 bp long, respectively, and contain several seed-specific motifs, such as AACA motif, ACGT element, E-Boxes, SEF binding sites and GCN4 motif. Functional analysis of these promoters in transgenic Arabidopsis plants was investigated after fusing them with the β-glucuronidase (GUS) reporter gene. None of the promoters triggered GUS activity in any vegetative tissue, with the exception of early seedling cotyledons. HaFAD2-1 and HaAP10 promoters were tested along seed development from globular stage to mature seeds. GUS staining was restricted to embryonic tissue and quantitative fluorometric assays showed high activity values at the later stages of development. In this work we demonstrate that HaFAD2-1 promoter is as strong as 35S promoter even though it is a tissue-specific promoter and its activity derived just from the embryo, thus confirming that it can be considered a strong highly specific seed promoter useful for biotechnology applications. © 2010 Springer-Verlag.

Registro:

Documento: Artículo
Título:Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)
Autor:Zavallo, D.; Bilbao, M.L.; Hopp, H.E.; Heinz, R.
Filiación:Instituto de Biotecnología, CNIA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Los Reseros y Nicolas Repeto, 1686 Hurlingham, Buenos Aires Province, Argentina
Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:Lipid transfer protein; Oleate desaturase; Seed promoter; Sunflower; Arabidopsis; Helianthus; Helianthus annuus; acyl coenzyme A desaturase; carrier protein; lipid transfer protein; oxidoreductase; Phosphatidylcholine desaturase; plant RNA; vegetable protein; Arabidopsis; article; gene expression profiling; gene expression regulation; genetics; molecular cloning; plant gene; plant seed; promoter region; sunflower; transgenic plant; Arabidopsis; Carrier Proteins; Cloning, Molecular; Fatty Acid Desaturases; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Plant; Helianthus; Oxidoreductases Acting on CH-CH Group Donors; Plant Proteins; Plants, Genetically Modified; Promoter Regions, Genetic; RNA, Plant; Seeds
Año:2010
Volumen:29
Número:3
Página de inicio:239
Página de fin:248
DOI: http://dx.doi.org/10.1007/s00299-010-0816-x
Título revista:Plant Cell Reports
Título revista abreviado:Plant Cell Rep.
ISSN:07217714
CODEN:PCRPD
CAS:acyl coenzyme A desaturase, 9014-34-0; carrier protein, 80700-39-6; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; Carrier Proteins; Fatty Acid Desaturases, 1.14.19.-; Oxidoreductases Acting on CH-CH Group Donors, 1.3.-; Phosphatidylcholine desaturase, 1.3.1.35; Plant Proteins; RNA, Plant; lipid transfer protein
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07217714_v29_n3_p239_Zavallo

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Citas:

---------- APA ----------
Zavallo, D., Bilbao, M.L., Hopp, H.E. & Heinz, R. (2010) . Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.). Plant Cell Reports, 29(3), 239-248.
http://dx.doi.org/10.1007/s00299-010-0816-x
---------- CHICAGO ----------
Zavallo, D., Bilbao, M.L., Hopp, H.E., Heinz, R. "Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)" . Plant Cell Reports 29, no. 3 (2010) : 239-248.
http://dx.doi.org/10.1007/s00299-010-0816-x
---------- MLA ----------
Zavallo, D., Bilbao, M.L., Hopp, H.E., Heinz, R. "Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)" . Plant Cell Reports, vol. 29, no. 3, 2010, pp. 239-248.
http://dx.doi.org/10.1007/s00299-010-0816-x
---------- VANCOUVER ----------
Zavallo, D., Bilbao, M.L., Hopp, H.E., Heinz, R. Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.). Plant Cell Rep. 2010;29(3):239-248.
http://dx.doi.org/10.1007/s00299-010-0816-x