Artículo

Rojas, D.A.; Perez-Munizaga, D.A.; Centanin, L.; Antonelli, M.; Wappner, P.; Allende, M.L.; Reyes, A.E. "Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish" (2007) Gene Expression Patterns. 7(3):339-345
La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveling the molecular mechanisms that mediate regulation of HIF proteins by oxygen tension has been reported, but our knowledge on their developmental regulation during embryogenesis in model organisms is limited. Expression of hif-1α and hif-2α genes has been characterized during normal mouse development and they were found to be expressed from stages E7.5, later in E9.5 and E15.5 in several different tissues such as the brain, heart and blood vessels. However, there is no detailed temporal information on their expression at other embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe the cloning and detailed expression pattern of zebrafish hif-1α and hif-2α genes. Sequence analysis revealed that zebrafish Hif proteins are highly homologous to other vertebrate orthologues. Zebrafish hif-1α and hif-2α are both expressed throughout development in discrete territories in a dynamic pattern. Interestingly, in the notochord the expression of hif-1α is switched off, while hif-2α transcription is turned on, signifying that the two genes might have partially overlapping, although non-redundant functions in development. This is the first time that a detailed comparison of the expression of hif-1α and hif-2α is directly assessed in a vertebrate model system throughout development. © 2006 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish
Autor:Rojas, D.A.; Perez-Munizaga, D.A.; Centanin, L.; Antonelli, M.; Wappner, P.; Allende, M.L.; Reyes, A.E.
Filiación:Facultad de Ciencias de la Salud, Universidad Diego Portales, Av. Ejército Libertador 141, Santiago, Chile
Millennium Nucleus in Developmental Biology, Departamento Biología, Facultad de Ciencias, Casilla 653, Santiago, Chile
Instituto Leloir, FCEyN-Universidad de Buenos Aires, Patricias Argentinas 435, Buenos Aires, 1405, Argentina
Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago, Chile
Palabras clave:Blood vessels; Brain; Cartilage; Dorsal aorta; Hypoxia-inducible factor; Intersegmental vessels; Neuromasts; Notochord; Optic tectum; Ventricle epithelia; Zebrafish; hypoxia inducible factor 1alpha; hypoxia inducible factor 2alpha; messenger RNA; oxygen; basic helix loop helix transcription factor; endothelial PAS domain containing protein 1; endothelial PAS domain-containing protein 1; hypoxia inducible factor 1alpha; zebrafish protein; angiogenesis; animal cell; article; blood vessel; brain tissue; controlled study; developmental stage; embryo; embryo development; expressed sequence tag; gene expression; gene expression regulation; gene function; genetic transcription; heart; heart development; hypoxia; molecular cloning; molecular mechanics; nervous system development; nonhuman; notochord; nucleotide sequence; oxygen tension; phylogenetic tree; priority journal; protein domain; reverse transcription polymerase chain reaction; sequence analysis; sequence homology; species difference; vertebrate; zebra fish; animal; animal embryo; antibody specificity; gene expression profiling; gene expression regulation; genetics; in situ hybridization; metabolism; molecular cloning; phylogeny; prenatal development; zebra fish; Danio rerio; Vertebrata; Animals; Basic Helix-Loop-Helix Transcription Factors; Cloning, Molecular; Embryo, Nonmammalian; Gene Expression Profiling; Gene Expression Regulation, Developmental; Hypoxia-Inducible Factor 1, alpha Subunit; In Situ Hybridization; Organ Specificity; Phylogeny; Zebrafish; Zebrafish Proteins
Año:2007
Volumen:7
Número:3
Página de inicio:339
Página de fin:345
DOI: http://dx.doi.org/10.1016/j.modgep.2006.08.002
Título revista:Gene Expression Patterns
Título revista abreviado:Gene Expr. Patterns
ISSN:1567133X
CODEN:GEPEA
CAS:oxygen, 7782-44-7; Basic Helix-Loop-Helix Transcription Factors; Hypoxia-Inducible Factor 1, alpha Subunit; Zebrafish Proteins; endothelial PAS domain-containing protein 1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1567133X_v7_n3_p339_Rojas

Referencias:

  • Bever, M.M., Fekete, D.M., Atlas of the developing inner ear in zebrafish (2002) Dev. Dyn., 223, pp. 536-543
  • Bruick, R.K., Oxygen sensing in the hypoxic response pathway: regulation of the hypoxia-inducible transcription factor (2003) Genes Dev., 17, pp. 2614-2623
  • Catron, T., Mendiola, M.A., Smith, S.M., Born, J., Walker, M.K., Hypoxia regulates avian cardiac Arnt and HIF-1alpha mRNA expression (2001) Biochem. Biophys. Res. Commun., 282, pp. 602-607
  • Chen, W.Y., John, J.A., Lin, C.H., Chang, C.Y., Molecular cloning and developmental expression of zinc finger transcription factor MTF-1 gene in zebrafish, Danio rerio (2002) Biochem. Biophys. Res. Commun., 291, pp. 798-805
  • Compernolle, V., Brusselmans, K., Acker, T., Hoet, P., Tjwa, M., Beck, H., Plaisance, S., Carmeliet, P., Loss of HIF-2alpha and inhibition of VEGF impair fetal lung maturation, whereas treatment with VEGF prevents fatal respiratory distress in premature mice (2002) Nat. Med., 8, pp. 702-710
  • Elvert, G., Lanz, S., Kappel, A., Flamme, I., mRNA cloning and expression studies of the quail homologue of HIF-2alpha (1999) Mech. Dev., 87, pp. 193-197
  • Ema, M., Taya, S., Yokotani, N., Sogawa, K., Matsuda, Y., Fujii-Kuriyama, Y., A novel bHLH-PAS factor with close sequence similarity to hypoxia-inducible factor 1alpha regulates the VEGF expression and is potentially involved in lung and vascular development (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 4273-4278
  • Flamme, I., Frohlich, T., von Reutern, M., Kappel, A., Damert, A., Risau, W., HRF, a putative basic helix-loop-helix-PAS-domain transcription factor is closely related to hypoxia-inducible factor-1 alpha and developmentally expressed in blood vessels (1997) Mech. Dev., 63, pp. 51-60
  • Haffter, P., Granato, M., Brand, M., Mullins, M.C., Hammerschmidt, M., Kane, D.A., Odenthal, J., Nusslein-Volhard, C., The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio (1996) Development, 123, pp. 1-36
  • Iyer, N.V., Kotch, L.E., Agani, F., Leung, S.W., Laughner, E., Wenger, R.H., Gassmann, M., Semenza, G.L., Cellular and developmental control of O2 homeostasis by hypoxia-inducible factor 1 alpha (1998) Genes Dev., 12, pp. 149-162
  • Iyer, N.V., Leung, S.W., Semenza, G.L., The human hypoxia-inducible factor 1alpha gene: HIF1A structure and evolutionary conservation (1998) Genomics, 52, pp. 159-165
  • Jain, S., Maltepe, E., Lu, M.M., Simon, C., Bradfield, C.A., Expression of ARNT, ARNT2, HIF1 alpha, HIF2 alpha and Ah receptor mRNAs in the developing mouse (1998) Mech. Dev., 73, pp. 117-123
  • Jowett, T., Lettice, L., Whole-mount in situ hybridizations on zebrafish embryos using a mixture of digoxigenin- and fluorescein-labelled probes (1994) Trends Genet., 10, pp. 73-74
  • Kimmel, C.B., Ballard, W.W., Kimmel, S.R., Ullmann, B., Schilling, T.F., Stages of embryonic development of the zebrafish (1995) Dev. Dyn., 203, pp. 253-310
  • Kotch, L.E., Iyer, N.V., Laughner, E., Semenza, G.L., Defective vascularization of HIF-1alpha-null embryos is not associated with VEGF deficiency but with mesenchymal cell death (1999) Dev. Biol., 209, pp. 254-267
  • Lawson, N.D., Weinstein, B.M., In vivo imaging of embryonic vascular development using transgenic zebrafish (2002) Dev. Biol., 248, pp. 307-318
  • Lee, Y.M., Jeong, C.H., Koo, S.Y., Son, M.J., Song, H.S., Bae, S.K., Raleigh, J.A., Kim, K.W., Determination of hypoxic region by hypoxia marker in developing mouse embryos in vivo: a possible signal for vessel development (2001) Dev. Dyn., 220, pp. 175-186
  • Lee, J.W., Bae, S.H., Jeong, J.W., Kim, S.H., Kim, K.W., Hypoxia-inducible factor (HIF-1)alpha: its protein stability and biological functions (2004) Exp. Mol. Med., 36, pp. 1-12
  • Luo, G., Gu, Y.Z., Jain, S., Chan, W.K., Carr, K.M., Hogenesch, J.B., Bradfield, C.A., Molecular characterization of the murine Hif-1 alpha locus (1997) Gene Expr., 6, pp. 287-299
  • Mackenzie, N.C., Brito, M., Reyes, A.E., Allende, M.L., Cloning, expression pattern and essentiality of the high-affinity copper transporter 1 (ctr1) gene in zebrafish (2004) Gene, 328, pp. 113-120
  • Madan, A., Varma, S., Cohen, H.J., Developmental stage-specific expression of the alpha and beta subunits of the HIF-1 protein in the mouse and human fetus (2002) Mol. Genet. Metab., 75, pp. 244-249
  • Maltepe, E., Schmidt, J.V., Baunoch, D., Bradfield, C.A., Simon, M.C., Abnormal angiogenesis and responses to glucose and oxygen deprivation in mice lacking the protein ARNT (1997) Nature, 386, pp. 403-407
  • Peng, J., Zhang, L., Drysdale, L., Fong, G.H., The transcription factor EPAS-1/hypoxia-inducible factor 2alpha plays an important role in vascular remodeling (2000) Proc. Natl. Acad. Sci. USA, 97, pp. 8386-8391
  • Pugh, C.W., Ratcliffe, P.J., Regulation of angiogenesis by hypoxia: role of the HIF system (2003) Nat. Med., 9, pp. 677-684
  • Royer, C., Lachuer, J., Crouzoulon, G., Roux, J., Peyronnet, J., Mamet, J., Pequignot, J., Dalmaz, Y., Effects of gestational hypoxia on mRNA levels of Glut3 and Glut4 transporters, hypoxia inducible factor-1 and thyroid hormone receptors in developing rat brain (2000) Brain Res., 856, pp. 119-128
  • Ryan, H.E., Lo, J., Johnson, R.S., HIF-1 alpha is required for solid tumor formation and embryonic vascularization (1998) EMBO J., 17, pp. 3005-3015
  • Schipani, E., Ryan, H.E., Didrickson, S., Kobayashi, T., Knight, M., Johnson, R.S., Hypoxia in cartilage: HIF-1alpha is essential for chondrocyte growth arrest and survival (2001) Genes Dev., 15, pp. 2865-2876
  • Schulte-Merker, S., Looking at embryos (2002) Zebrafish, pp. 39-58. , Nüsslein-Volhard C., and Dahm R. (Eds), Oxford University Press, New York
  • Semenza, G.L., Regulation of mammalian O 2 homeostasis by hypoxia-inducible factor 1 (1999) Annu. Rev. Cell Dev. Biol., 15, pp. 551-578
  • Semenza, G.L., HIF-1 and mechanisms of hypoxia sensing (2001) Curr. Opin. Cell Biol., 13, pp. 167-171
  • Sipe, C.W., Gruber, E.J., Saha, M.S., Short upstream region drives dynamic expression of hypoxia-inducible factor 1alpha during Xenopus development (2004) Dev. Dyn., 230, pp. 229-238
  • Tian, H., Hammer, R.E., Matsumoto, A.M., Russell, D.W., McKnight, S.L., The hypoxia-responsive transcription factor EPAS1 is essential for catecholamine homeostasis and protection against heart failure during embryonic development (1998) Genes Dev., 12, pp. 3320-3324
  • Ton, C., Stamatiou, D., Liew, C.C., Gene expression profile of zebrafish exposed to hypoxia during development (2003) Physiol. Genomics, 13, pp. 97-106
  • Wang, G.L., Semenza, G.L., Purification and characterization of hypoxia-inducible factor 1 (1995) J. Biol. Chem., 270, pp. 1230-1237
  • Westerfield, M., (1995) The Zebrafish Book. fourth ed., , University of Oregon Press, Eugene

Citas:

---------- APA ----------
Rojas, D.A., Perez-Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L. & Reyes, A.E. (2007) . Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish. Gene Expression Patterns, 7(3), 339-345.
http://dx.doi.org/10.1016/j.modgep.2006.08.002
---------- CHICAGO ----------
Rojas, D.A., Perez-Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L., et al. "Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish" . Gene Expression Patterns 7, no. 3 (2007) : 339-345.
http://dx.doi.org/10.1016/j.modgep.2006.08.002
---------- MLA ----------
Rojas, D.A., Perez-Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L., et al. "Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish" . Gene Expression Patterns, vol. 7, no. 3, 2007, pp. 339-345.
http://dx.doi.org/10.1016/j.modgep.2006.08.002
---------- VANCOUVER ----------
Rojas, D.A., Perez-Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L., et al. Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish. Gene Expr. Patterns. 2007;7(3):339-345.
http://dx.doi.org/10.1016/j.modgep.2006.08.002