Rojas, D.A.; Perez-Munizaga, D.A.; Centanin, L.; Antonelli, M.; Wappner, P.; Allende, M.L.; Reyes, A.E. "Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish" (2007) Gene Expression Patterns. 7(3):339-345
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Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveling the molecular mechanisms that mediate regulation of HIF proteins by oxygen tension has been reported, but our knowledge on their developmental regulation during embryogenesis in model organisms is limited. Expression of hif-1α and hif-2α genes has been characterized during normal mouse development and they were found to be expressed from stages E7.5, later in E9.5 and E15.5 in several different tissues such as the brain, heart and blood vessels. However, there is no detailed temporal information on their expression at other embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe the cloning and detailed expression pattern of zebrafish hif-1α and hif-2α genes. Sequence analysis revealed that zebrafish Hif proteins are highly homologous to other vertebrate orthologues. Zebrafish hif-1α and hif-2α are both expressed throughout development in discrete territories in a dynamic pattern. Interestingly, in the notochord the expression of hif-1α is switched off, while hif-2α transcription is turned on, signifying that the two genes might have partially overlapping, although non-redundant functions in development. This is the first time that a detailed comparison of the expression of hif-1α and hif-2α is directly assessed in a vertebrate model system throughout development. © 2006 Elsevier B.V. All rights reserved.


Documento: Artículo
Título:Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish
Autor:Rojas, D.A.; Perez-Munizaga, D.A.; Centanin, L.; Antonelli, M.; Wappner, P.; Allende, M.L.; Reyes, A.E.
Filiación:Facultad de Ciencias de la Salud, Universidad Diego Portales, Av. Ejército Libertador 141, Santiago, Chile
Millennium Nucleus in Developmental Biology, Departamento Biología, Facultad de Ciencias, Casilla 653, Santiago, Chile
Instituto Leloir, FCEyN-Universidad de Buenos Aires, Patricias Argentinas 435, Buenos Aires, 1405, Argentina
Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago, Chile
Palabras clave:Blood vessels; Brain; Cartilage; Dorsal aorta; Hypoxia-inducible factor; Intersegmental vessels; Neuromasts; Notochord; Optic tectum; Ventricle epithelia; Zebrafish; hypoxia inducible factor 1alpha; hypoxia inducible factor 2alpha; messenger RNA; oxygen; basic helix loop helix transcription factor; endothelial PAS domain containing protein 1; endothelial PAS domain-containing protein 1; hypoxia inducible factor 1alpha; zebrafish protein; angiogenesis; animal cell; article; blood vessel; brain tissue; controlled study; developmental stage; embryo; embryo development; expressed sequence tag; gene expression; gene expression regulation; gene function; genetic transcription; heart; heart development; hypoxia; molecular cloning; molecular mechanics; nervous system development; nonhuman; notochord; nucleotide sequence; oxygen tension; phylogenetic tree; priority journal; protein domain; reverse transcription polymerase chain reaction; sequence analysis; sequence homology; species difference; vertebrate; zebra fish; animal; animal embryo; antibody specificity; gene expression profiling; gene expression regulation; genetics; in situ hybridization; metabolism; molecular cloning; phylogeny; prenatal development; zebra fish; Danio rerio; Vertebrata; Animals; Basic Helix-Loop-Helix Transcription Factors; Cloning, Molecular; Embryo, Nonmammalian; Gene Expression Profiling; Gene Expression Regulation, Developmental; Hypoxia-Inducible Factor 1, alpha Subunit; In Situ Hybridization; Organ Specificity; Phylogeny; Zebrafish; Zebrafish Proteins
Página de inicio:339
Página de fin:345
Título revista:Gene Expression Patterns
Título revista abreviado:Gene Expr. Patterns
CAS:oxygen, 7782-44-7; Basic Helix-Loop-Helix Transcription Factors; Hypoxia-Inducible Factor 1, alpha Subunit; Zebrafish Proteins; endothelial PAS domain-containing protein 1


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---------- APA ----------
Rojas, D.A., Perez-Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L. & Reyes, A.E. (2007) . Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish. Gene Expression Patterns, 7(3), 339-345.
---------- CHICAGO ----------
Rojas, D.A., Perez-Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L., et al. "Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish" . Gene Expression Patterns 7, no. 3 (2007) : 339-345.
---------- MLA ----------
Rojas, D.A., Perez-Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L., et al. "Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish" . Gene Expression Patterns, vol. 7, no. 3, 2007, pp. 339-345.
---------- VANCOUVER ----------
Rojas, D.A., Perez-Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L., et al. Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish. Gene Expr. Patterns. 2007;7(3):339-345.