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Gimenez, A.M.; Gesumaría, M.C.; Schoijet, A.C.; Alonso, G.D.; Flawiá, M.M.; Racagni, G.E.; Machado, E.E. "Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes" (2015) Molecular and Biochemical Parasitology. 203(1-2):14-24
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Abstract:

Phosphatidylinositol (PtdIns) metabolism through phosphatidylinositol kinase (PIKs) activities plays a central role in different signaling pathways. In Trypanosoma cruzi, causative agent of Chagas disease, PIKs have been proposed as target for drug design in order to combat this pathogen. In this work, we studied the classes of PI4K, PIPK and PI3K that could participate in signaling pathways in T. cruzi epimastigote forms. For this reason, we analyzed their enzymatic parameters and detailed responses to avowed kinase inhibitors (adenosine, sodium deoxycholate, wortmannin and LY294002) and activators (Ca2+, phosphatidic acid, spermine and heparin). Our results suggest the presence and activity of a class III PI4K, a class I PIPK, a class III PI3K previously described (TcVps34) and a class I PI3K. Class I PI3K enzyme, here named TcPI3K, was cloned and expressed in a bacterial system, and their product was tested for kinase activity. The possible participation of TcPI3K in central cellular events of the parasite is also discussed. © 2015 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes
Autor:Gimenez, A.M.; Gesumaría, M.C.; Schoijet, A.C.; Alonso, G.D.; Flawiá, M.M.; Racagni, G.E.; Machado, E.E.
Filiación:Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, X5800, Argentina
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, Ciudad Autónoma de Buenos Aires, C1428ADN, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Kinase activity; Phosphatidylinositol-3 kinase; Phosphoinositide kinase; Phospholipid signaling; T. cruzi epimastigotes; TcPI3K; 2 morpholino 8 phenylchromone; adenosine; calcium ion; deoxycholate sodium; heparin; phosphatidic acid; phosphatidylinositol 3 kinase; phosphatidylinositol 4 phosphate kinase; phosphatidylinositol 4,5 bisphosphate 3 kinase; phosphatidylinositol kinase; protozoal protein; spermine; wortmannin; enzyme activator; enzyme inhibitor; phosphatidylinositol; protozoal protein; Article; controlled study; cytosolic fraction; enzyme activity; enzyme phosphorylation; epimastigote; molecular cloning; nonhuman; Northern blotting; phylogeny; priority journal; protein expression; signal transduction; Trypanosoma cruzi; Western blotting; antagonists and inhibitors; Chagas disease; classification; drug design; enzymology; growth, development and aging; human; metabolism; parasitology; phosphorylation; Trypanosoma cruzi; Chagas Disease; Cloning, Molecular; Drug Design; Enzyme Activators; Enzyme Inhibitors; Humans; Phosphatidylinositol 3-Kinases; Phosphatidylinositols; Phosphorylation; Phylogeny; Protozoan Proteins; Signal Transduction; Trypanosoma cruzi
Año:2015
Volumen:203
Número:1-2
Página de inicio:14
Página de fin:24
DOI: http://dx.doi.org/10.1016/j.molbiopara.2015.10.002
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:2 morpholino 8 phenylchromone, 154447-36-6; adenosine, 58-61-7; calcium ion, 14127-61-8; deoxycholate sodium, 302-95-4; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; phosphatidylinositol 3 kinase, 115926-52-8; phosphatidylinositol 4 phosphate kinase, 9032-61-5; phosphatidylinositol 4,5 bisphosphate 3 kinase, 103843-30-7; phosphatidylinositol kinase, 37205-54-2; spermine, 306-67-2, 71-44-3; wortmannin, 19545-26-7; Enzyme Activators; Enzyme Inhibitors; Phosphatidylinositol 3-Kinases; Phosphatidylinositols; Protozoan Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v203_n1-2_p14_Gimenez

Referencias:

  • Chagas, C., Nova tripanozomiaze humana. Estudos sobre a morfolojia e o ciclo evolutivo do Schizotrypanum cruzi n. Gen., n. Sp., ajente etiolojico de nova entidade morbida do homem (1909) Mem. Inst. Oswaldo Cruz, 1, pp. 159-218
  • World Health Organization (W.H.O.), , http://www.who.int/mediacentre/factsheets/fs340/en/, web page, (consulted 04.10.15)
  • Parsons, M., Ruben, L., Pathways involved in environmental sensing in trypanosomatids (2000) Parasitol. Today, 16 (FEBRUARY 2), pp. 56-62. , PubMed PMID: 10652488
  • Machado-Domenech, E.E.M., Garcia, M., Garrido, M.N., Racagni, G., Phospholipids of Trypanosoma cruzi - Increase of polyphosphoinositides and phosphatidic-acid after cholinergic stimulation (1992) Fems Microbiol. Lett., 95 (AUGUST 2-3), pp. 267-270. , PubMed PMID: WOS: A1992JJ66600025
  • Garrido, M.N., Bollo, M.I., Machado-Domenech, E.E., Biphasic and dose-dependent accumulation of INSP3 in Trypanosoma cruzi stimulated by a synthetic peptide carrying a chicken alpha d-globin fragment (1996) Cell. Mol. Biol., 42 (SEPTEMBER 6), pp. 859-864. , PubMed PMID: 8891353
  • Nozaki, T., Toh-E, A., Fujii, M., Yagisawa, H., Nakazawa, M., Takeuchi, T., Cloning and characterization of a gene encoding phosphatidyl inositol-specific phospholipase C from Trypanosoma cruzi (1999) Mol. Biochem. Parasitol., 102 (AUGUST 2), pp. 283-295. , PubMed PMID: 10498184
  • Furuya, T., Kashuba, C., Docampo, R., Moreno, S.N., A novel phosphatidylinositol-phospholipase C of Trypanosoma cruzi that is lipid modified and activated during trypomastigote to amastigote differentiation (2000) J. Biol. Chem., 275 (MARCH 9), pp. 6428-6438. , PubMed PMID: 10692446
  • Marchesini, N., Bollo, M., Hernandez, G., Garrido, M.N., Machado-Domenech, E.E., Cellular signalling in Trypanosoma cruzi: Biphasic behaviour of inositol phosphate cycle components evoked by carbachol (2002) Mol. Biochem. Parasitol., 120 (MARCH 1), pp. 83-91. , PubMed PMID: 11849708
  • Santander, V., Bollo, M., Machado-Domenech, E., Lipid kinases and Ca(2+) signaling in Trypanosoma cruzi stimulated by a synthetic peptide (2002) Biochem. Biophys. Res. Commun., 293 (APRIL 1), pp. 314-320. , PubMed PMID: 12054601
  • Vanhaesebroeck, B., Guillermet-Guibert, J., Graupera, M., Bilanges, B., The emerging mechanisms of isoform-specific PI3K signalling (2010) Nat. Rev. Mol. Cell Biol., 11 (MAY 5), pp. 329-341. , PubMed PMID: 20379207
  • Hall, B.S., Gabernet-Castello, C., Voak, A., Goulding, D., Natesan, S.K., Field, M.C., TbVps34, the trypanosome orthologue of Vps34, is required for Golgi complex segregation (2006) J. Biol. Chem., 281 (SEPTEMBER 37), pp. 27600-27612. , PubMed PMID: 16835237
  • Braga, M.V., De Souza, W., Effects of protein kinase and phosphatidylinositol-3 kinase inhibitors on growth and ultrastructure of Trypanosoma cruzi (2006) Fems Microbiol. Lett., 256 (MARCH 2), pp. 209-216. , PubMed PMID: 16499608
  • Diaz-Gonzalez, R., Kuhlmann, F.M., Galan-Rodriguez, C., Madeira da Silva, L., Saldivia, M., Karver, C.E., The susceptibility of trypanosomatid pathogens to PI3/mTOR kinase inhibitors affords a new opportunity for drug repurposing (2011) PLoS Negl. Trop. Dis., 5 (AUGUST 8), p. e1297. , PubMed PMID: 21886855, Pubmed Central PMCID: 3160303
  • Demmel, L., Schmidt, K., Lucast, L., Havlicek, K., Zankel, A., Koestler, T., The endocytic activity of the flagellar pocket in Trypanosoma brucei is regulated by an adjacent phosphatidylinositol phosphate kinase (2014) J. Cell Sci., 127 (MAY PT. 10), pp. 2351-2364. , PubMed PMID: 24639465, Pubmed Central PMCID: 4021478
  • Bahia, D., Oliveira, L.M., Lima, F.M., Oliveira, P., Silveira, J.F., Mortara, R.A., The TryPIKinome of five human pathogenic trypanosomatids: Trypanosoma brucei, Trypanosoma cruzi, Leishmania major, Leishmania braziliensis and Leishmania infantum - New tools for designing specific inhibitors (2009) Biochem. Biophys. Res. Commun., 390 (DECEMBER 3), pp. 963-970. , PubMed PMID: 19852933
  • Lima, F.M., Oliveira, P., Mortara, R.A., Silveira, J.F., Bahia, D., The challenge of Chagas' disease: Has the human pathogen, Trypanosoma cruzi, learned how to modulate signaling events to subvert host cells? (2010) New Biotechnol., 27 (DECEMBER 6), pp. 837-843. , PubMed PMID: 20172059
  • Oliveira, P., Lima, F.M., Cruz, M.C., Ferreira, R.C., Sanchez-Flores, A., Cordero, E.M., Trypanosoma cruzi: Genome characterization of phosphatidylinositol kinase gene family (PIK and PIK-related) and identification of a novel PIK gene (2014) Infect. Genet. Evol.: J. Mol. Epidemiol. Evolut. Genet. Infect. Dis., 25 (JULY), pp. 157-165. , PubMed PMID: 24727645
  • Schoijet, A.C., Miranda, K., Girard-Dias, W., De Souza, W., Flawia, M.M., Torres, H.N., A Trypanosoma cruzi phosphatidylinositol 3-kinase (TcVps34) is involved in osmoregulation and receptor-mediated endocytosis (2008) J. Biol. Chem., 283 (NOVEMBER 46), pp. 31541-31550. , PubMed PMID: 18801733, Pubmed Central PMCID: 2581564
  • Rodgers, M.J., Albanesi, J.P., Phillips, M.A., Phosphatidylinositol 4-kinase III-beta is required for Golgi maintenance and cytokinesis in Trypanosoma brucei (2007) Eukaryot. Cell, 6 (JULY 7), pp. 1108-1118. , PubMed PMID: 17483288, Pubmed Central PMCID: 1951100
  • Bonansea, S., Usorach, M., Gesumaria, M.C., Santander, V., Gimenez, A.M., Bollo, M., Stress response to high osmolarity in Trypanosoma cruzi epimastigotes (2012) Arch. Biochem. Biophys., 527 (NOVEMBER 1), pp. 6-15. , PubMed PMID: 22884762
  • Gimenez, A.M., Santander, V.S., Villasuso, A.L., Pasquare, S.J., Giusto, N.M., Machado, E.E., Regulation of phosphatidic acid levels in Trypanosoma cruzi (2011) Lipids, 46 (OCTOBER 10), pp. 969-979. , PubMed PMID: 21667213
  • Warren, L.G., Metabolism of Schizotrypanum cruzi Chagas. I. Effect of culture age and substrate concentration on respiratory rate (1960) J. Parasitol., 46 (OCTOBER), pp. 529-539. , PubMed PMID: 13783227
  • El-Sayed, N.M., Myler, P.J., Bartholomeu, D.C., Nilsson, D., Aggarwal, G., Tran, A.N., The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease (2005) Science, 309 (JULY 5733), pp. 409-415. , PubMed PMID: 16020725
  • Stack, J.H., Herman, P.K., Schu, P.V., Emr, S.D., A membrane-associated complex containing the Vps15 protein kinase and the Vps34 PI 3-kinase is essential for protein sorting to the yeast lysosome-like vacuole (1993) EMBO J., 12 (MAY 5), pp. 2195-2204. , PubMed PMID: 8387919, Pubmed Central PMCID: 413440
  • Mak, L.H., Woscholski, R., Targeting PTEN using small molecule inhibitors (2015) Methods, 77-78 (MAY), pp. 63-68. , PubMed PMID: 25747336
  • Spinelli, L., Lindsay, Y.E., Leslie, N.R., PTEN inhibitors: An evaluation of current compounds (2015) Adv. Biol. Regul., 57 (JANUARY), pp. 102-111. , PubMed PMID: 25446882
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72 (MAY), pp. 248-254. , PubMed PMID: 942051
  • Stubbs, E.B., Jr., Kelleher, J.A., Sun, G.Y., Phosphatidylinositol kinase, phosphatidylinositol-4-phosphate kinase and diacylglycerol kinase activities in rat brain subcellular fractions (1988) Biochim. Biophys. Acta, 958 (2), pp. 247-254. , PubMed PMID: 2827783
  • Marchesini, N., Santander, V., MacHado-Domenech, E., Diacylglycerol pyrophosphate: A novel metabolite in the Trypanosoma cruzi phosphatidic acid metabolism (1998) FEBS Lett., 436 (OCTOBER 3), pp. 377-381. , PubMed PMID: 9801152
  • Walsh, J.P., Caldwell, K.K., Majerus, P.W., Formation of phosphatidylinositol3-phosphate by isomerization from phosphatidylinositol 4-phosphate (1991) Proc. Natl. Acad. Sci. U. S. A., 88 (OCYOBER 20), pp. 9184-9187. , PubMed PMID: 1656463, Pubmed Central PMCID: 52677
  • Alonso, G.D., Schoijet, A.C., Torres, H.N., Flawia, M.M., TcrPDEA1 a cAMP-specific phosphodiesterase with atypical pharmacological properties from Trypanosoma cruzi (2007) Mol. Biochem. Parasitol., 152 (MARCH 1), pp. 72-79. , PubMed PMID: 17222469
  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227 (AUGUST 5259), pp. 680-685. , PubMed PMID: 5432063
  • Balla, A., Balla, T., Phosphatidylinositol 4-kinases: Old enzymes with emerging functions (2006) Trends Cell Biol., 16 (JULY 7), pp. 351-361. , PubMed PMID: 16793271
  • Toker, A., Phosphoinositides and signal transduction (2002) Cellular Mol. Life Sci.: CMLS, 59 (MAY 5), pp. 761-779. , PubMed PMID: 12088277
  • Bao, Y., Weiss, L.M., Braunstein, V.L., Huang, H., Role of protein kinase A in Trypanosoma cruzi (2008) Infect. Immun., 76 (OCTOBER 10), pp. 4757-4763. , PubMed PMID: 18694966, Pubmed Central PMCID: 2546855
  • Shemarova, I.V., Phosphoinositide signaling in unicellular eukaryotes (2007) Crit. Rev. Microbiol., 33 (3), pp. 141-156. , PubMed PMID: 17653984
  • Bazenet, C.E., Ruano, A.R., Brockman, J.L., Anderson, R.A., The human erythrocyte contains two forms of phosphatidylinositol-4-phosphate 5-kinase which are differentially active toward membranes (1990) J. Biol. Chem., 265 (OCTOBER 29), pp. 18012-18022. , PubMed PMID: 2170402
  • Cockcroft, S., Phosphatidic acid regulation of phosphatidylinositol 4-phosphate 5-kinases (2009) Biochim. Biophys. Acta, 1791 (SEPTEMBER 9), pp. 905-912. , PubMed PMID: 19298865
  • Leondaritis, G., Tiedtke, A., Galanopoulou, D., D-3 phosphoinositides of the ciliate Tetrahymena: Characterization and study of their regulatory role in lysosomal enzyme secretion (2005) Biochim. Biophys. Acta, 1745 (SEPTEMBER 3), pp. 330-341. , PubMed PMID: 16081170
  • King, J.S., Insall, R.H., Chemotaxis: Finding the way forward with Dictyostelium (2009) Trends Cell Biol., 19 (OCTOBER 10), pp. 523-530. , PubMed PMID: 19733079
  • Davies, S.P., Reddy, H., Caivano, M., Cohen, P., Specificity and mechanism of action of some commonly used protein kinase inhibitors (2000) Biochem. J., 351 (OCTOBER PT. 1), pp. 95-105. , PubMed PMID: 10998351, Pubmed Central PMCID: 1221339
  • Cox, S.S., Van Der Giezen, M., Tarr, S.J., Crompton, M.R., Tovar, J., Evidence from bioinformatics, expression and inhibition studies of phosphoinositide-3 kinase signalling in Giardia intestinalis (2006) BMC Microbiol., 6, p. 45. , PubMed PMID: 16707026, Pubmed Central PMCID: 1483827
  • Kovács, P.P.E., Phosphatidylinositol 3-kinase-like activity in tetrahymena. Effects of Wortmannin and LY 294002 (2003) Acta Protozool., 42, pp. 277-285
  • Hernandez, Y., Zamora, G., Ray, S., Chapoy, J., Chavez, E., Valvarde, R., Transcriptional analysis of three major putative phosphatidylinositol kinase genes in a parasitic protozoan, Giardia lamblia (2007) J. Eukaryot. Microbiol., 54 (1 JANUARY), pp. 29-32. , PubMed PMID: 17300515, Pubmed Central PMCID: 3124632
  • Walker, E.H., Pacold, M.E., Perisic, O., Stephens, L., Hawkins, P.T., Wymann, M.P., Structural determinants of phosphoinositide 3-kinase inhibition by wortmannin, LY294002, quercetin, myricetin, and staurosporine (2000) Mol. Cell, 6 (OCTOBER 4), pp. 909-919. , PubMed PMID: 11090628
  • Brennwald, P., Novick, P., Interactions of three domains distinguishing the Ras-related GTP-binding proteins Ypt1 and Sec4 (1993) Nature, 362 (APRIL 6420), pp. 560-563. , PubMed PMID: 8464498
  • Mauricio De Mendonca, S.M., Nepomuceno da Silva, J.L., Cunha e-Silva, N., De Souza, W., Gazos Lopes, U., Characterization of a Rab11 homologue in Trypanosoma cruzi (2000) Gene, 243 (2 FEBRUARY 1), pp. 179-185. , PubMed PMID: 10675626
  • Araripe, J.R., Cunha Silva, E.N.L., Leal, S.T., De Souza, W., Rondinelli, E., Trypanosoma cruzi: TcRAB7 protein is localized at the Golgi apparatus in epimastigotes (2004) Biochem. Biophys. Res. Commun., 321 (AUGUST 2), pp. 397-402. , PubMed PMID: 15358190
  • Araripe, J.R., Ramos, F.P., Cunha Silva, E.N.L., Urmenyi, T.P., Silva, R., Leite Fontes, C.F., Characterization of a RAB5 homologue in Trypanosoma cruzi (2005) Biochem. Biophys. Res. Commun., 329 (APRIL 2), pp. 638-645. , PubMed PMID: 15737633
  • Shemarova, I.V., CAMP-dependent signal pathways in unicellular eukaryotes (2009) Crit. Rev. Microbiol., 35 (1), pp. 23-42. , PubMed PMID: 19514907
  • Gonzales-Perdomo, M., Romero, P., Goldenberg, S., Cyclic AMP and adenylate cyclase activators stimulate Trypanosoma cruzi differentiation (1988) Exp. Parasitol., 66 (AUGUST 2), pp. 205-212. , PubMed PMID: 2840306
  • Jimenez, V., Dealing with environmental challenges: Mechanisms of adaptation in Trypanosoma cruzi (2014) Res. Microbiol., 165 (APRIL 3), pp. 155-165. , PubMed PMID: 24508488, Pubmed Central PMCID: 3997592
  • Minning, T.A., Weatherly, D.B., Atwood, J., III, Orlando, R., Tarleton, R.L., The steady-state transcriptome of the four major life-cycle stages of Trypanosoma cruzi (2009) BMC Genomics, 10, p. 370. , PubMed PMID: 19664227, Pubmed Central PMCID: 2907688
  • Pascuccelli, V., Labriola, C., Tellez-Inon, M.T., Parodi, A.J., Molecular and biochemical characterization of a protein kinase B from Trypanosoma cruzi (1999) Mol. Biochem. Parasitol., 102 (JULY 1), pp. 21-33. , PubMed PMID: 10477173

Citas:

---------- APA ----------
Gimenez, A.M., Gesumaría, M.C., Schoijet, A.C., Alonso, G.D., Flawiá, M.M., Racagni, G.E. & Machado, E.E. (2015) . Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes. Molecular and Biochemical Parasitology, 203(1-2), 14-24.
http://dx.doi.org/10.1016/j.molbiopara.2015.10.002
---------- CHICAGO ----------
Gimenez, A.M., Gesumaría, M.C., Schoijet, A.C., Alonso, G.D., Flawiá, M.M., Racagni, G.E., et al. "Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes" . Molecular and Biochemical Parasitology 203, no. 1-2 (2015) : 14-24.
http://dx.doi.org/10.1016/j.molbiopara.2015.10.002
---------- MLA ----------
Gimenez, A.M., Gesumaría, M.C., Schoijet, A.C., Alonso, G.D., Flawiá, M.M., Racagni, G.E., et al. "Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes" . Molecular and Biochemical Parasitology, vol. 203, no. 1-2, 2015, pp. 14-24.
http://dx.doi.org/10.1016/j.molbiopara.2015.10.002
---------- VANCOUVER ----------
Gimenez, A.M., Gesumaría, M.C., Schoijet, A.C., Alonso, G.D., Flawiá, M.M., Racagni, G.E., et al. Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes. Mol. Biochem. Parasitol. 2015;203(1-2):14-24.
http://dx.doi.org/10.1016/j.molbiopara.2015.10.002