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Ferreyra, R.G.; Burgardt, N.I.; Milikowski, D.; Melen, G.; Kornblihtt, A.R.; Dell' Angelica, E.C.; Santomé, J.A.; Ermácora, M.R. "A yeast sterol carrier protein with fatty-acid and fatty-acyl-CoA binding activity" (2006) Archives of Biochemistry and Biophysics. 453(2):197-206
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Abstract:

The 14-kDa sterol carrier protein 2 (SCP2) domain is present in Eukaria, Bacteria and Archaea, and has been implicated in the transport and metabolism of lipids. We report the cloning, expression, purification and physicochemical characterization of a SCP2 from the yeast Yarrowia lipolytica (YLSCP2). Analytical size-exclusion chromatography, circular dichroism and fluorescence spectra, indicate that recombinant YLSCP2 is a well-folded monomer. Thermal unfolding experiments show that SCP2 maximal stability is at pH 7.0-9.0. YLSCP2 binds cis-parinaric acid and palmitoyl-CoA with KD values of 81 ± 40 nM and 73 ± 33 nM, respectively, sustaining for the first time the binding of fatty acids and their CoA esters to a nonanimal SCP2. The role of yeast SCP2 and other lipid binding proteins in transport, storage and peroxisomal oxidation of fatty acids is discussed. © 2006 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:A yeast sterol carrier protein with fatty-acid and fatty-acyl-CoA binding activity
Autor:Ferreyra, R.G.; Burgardt, N.I.; Milikowski, D.; Melen, G.; Kornblihtt, A.R.; Dell' Angelica, E.C.; Santomé, J.A.; Ermácora, M.R.
Filiación:Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina
Department of Human Genetics, University of California, Los Angeles School of Medicine, Los Angeles, CA 90095-7088, United States
Palabras clave:Circular dichroism; Fatty-acid; Fatty-acyl-CoA; Lipid binding protein; Peroxisomes; Sterol carrier protein; Yarrowia lipolytica; Yeast; acyl coenzyme A; fatty acid; lipid; lipid binding protein; palmitoyl coenzyme A; parinaric acid; recombinant protein; sterol carrier protein 2; article; circular dichroism; fatty acid oxidation; fluorescence spectroscopy; gel permeation chromatography; molecular cloning; nonhuman; nucleotide sequence; peroxisome; priority journal; protein domain; protein expression; protein folding; protein function; protein purification; protein stability; Yarrowia lipolytica; Acyl-CoA Dehydrogenase; Amino Acid Sequence; Binding Sites; Carrier Proteins; Computer Simulation; Fatty Acids; Models, Chemical; Models, Molecular; Molecular Sequence Data; Protein Binding; Yarrowia; Yarrowia lipolytica
Año:2006
Volumen:453
Número:2
Página de inicio:197
Página de fin:206
DOI: http://dx.doi.org/10.1016/j.abb.2006.06.024
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:lipid, 66455-18-3; palmitoyl coenzyme A, 1763-10-6; parinaric acid, 18427-44-6; Acyl-CoA Dehydrogenase, EC 1.3.99.3; Carrier Proteins; Fatty Acids; sterol carrier proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00039861_v453_n2_p197_Ferreyra

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Citas:

---------- APA ----------
Ferreyra, R.G., Burgardt, N.I., Milikowski, D., Melen, G., Kornblihtt, A.R., Dell' Angelica, E.C., Santomé, J.A.,..., Ermácora, M.R. (2006) . A yeast sterol carrier protein with fatty-acid and fatty-acyl-CoA binding activity. Archives of Biochemistry and Biophysics, 453(2), 197-206.
http://dx.doi.org/10.1016/j.abb.2006.06.024
---------- CHICAGO ----------
Ferreyra, R.G., Burgardt, N.I., Milikowski, D., Melen, G., Kornblihtt, A.R., Dell' Angelica, E.C., et al. "A yeast sterol carrier protein with fatty-acid and fatty-acyl-CoA binding activity" . Archives of Biochemistry and Biophysics 453, no. 2 (2006) : 197-206.
http://dx.doi.org/10.1016/j.abb.2006.06.024
---------- MLA ----------
Ferreyra, R.G., Burgardt, N.I., Milikowski, D., Melen, G., Kornblihtt, A.R., Dell' Angelica, E.C., et al. "A yeast sterol carrier protein with fatty-acid and fatty-acyl-CoA binding activity" . Archives of Biochemistry and Biophysics, vol. 453, no. 2, 2006, pp. 197-206.
http://dx.doi.org/10.1016/j.abb.2006.06.024
---------- VANCOUVER ----------
Ferreyra, R.G., Burgardt, N.I., Milikowski, D., Melen, G., Kornblihtt, A.R., Dell' Angelica, E.C., et al. A yeast sterol carrier protein with fatty-acid and fatty-acyl-CoA binding activity. Arch. Biochem. Biophys. 2006;453(2):197-206.
http://dx.doi.org/10.1016/j.abb.2006.06.024