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Abstract:

We have investigated bacterial populations relevant to nitrification in a full-scale activated sludge plant receiving wastewater from a petroleum refinery showing unstable nitrification. Inhibition of ammonia oxidation was related to phenol concentration according to a model of non-competitive inhibition. While the number of ammonia-oxidizing bacteria (AOB) did not correlate with nitrification performance, the total number of nitrite-oxidizing bacteria (NOB) dropped considerably during periods of nitrite accumulation or no nitrification. Diversity of nitrifiers in the sludge of the full-scale facility was examined at a time of full nitrification with the construction of clone libraries of ammonia monooxygenase (amoA) gene and of the 16S rRNA gene of NOB. Nucleotide sequences of amoA gene belonged to one dominant population, associated with Nitrosomonas europaea, and to a minor population related to the Nitrosomonas nitrosa lineage. The majority of sequences retrieved in the NOB-like clone library also clustered within a single operational taxonomic unit. The high dominance of Nitrobacter over Nitrospira and the low diversity of nitrifying bacteria observed in this wastewater treatment plant might account for the increased risk of failure in the presence of disturbances. © 2010 Elsevier B.V.

Registro:

Documento: Artículo
Título:Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification
Autor:Figuerola, E.L.M.; Erijman, L.
Filiación:Instituto de Investigaciones en Ingeniería Genética y, Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Activated sludge; Ammonia-oxidizing bacteria; Nitrification; Nitrobacter; Phenol; 16S rRNA gene; Activated sludge; Activated sludge plants; Ammonia oxidation; Ammonia oxidizing bacteria; Bacterial population; Clone library; Competitive inhibition; Monooxygenases; Nitrifying bacteria; Nitrite accumulation; Nitrite-oxidizing bacteria; Nitrobacter; Nitrosomonas; Nitrosomonas europaea; Nitrospira; Nucleotide sequences; Operational taxonomic units; Phenol concentration; Risk of failure; Wastewater treatment plants; Ammonia; Bacteria; Bacteriology; Bioactivity; Chemical oxygen demand; Cloning; Denitrification; Nitration; Oxidation; Petroleum refineries; Phenols; Refining; RNA; Sewage pumping plants; Wastewater; Wastewater reclamation; Wastewater treatment; Water treatment plants; Nitrification; ammonia; DNA; nitrite; petroleum; phenol; RNA 16S; unspecific monooxygenase; ammonia monooxygenase; industrial waste; oxidoreductase; petroleum; RNA 16S; water pollutant; activated sludge; disturbance; enzyme activity; genetic analysis; inhibition; nitrification; nitrifying bacterium; oxidation; petroleum; phenol; refining industry; species diversity; taxonomy; waste facility; wastewater; water treatment; ammonia oxidizing bacteria; article; DNA extraction; gene sequence; gene targeting; microbial diversity; molecular cloning; nitrification; nitrifying bacterium; nitrite oxidizing bacterium; Nitrobacter; Nitrosomonas europaea; nonhuman; nucleotide sequence; oxidation; petrochemical industry; phylogeny; real time polymerase chain reaction; sludge; taxonomy; unindexed sequence; waste water management; Bacteria; bioremediation; genetics; industrial waste; isolation and purification; metabolism; prevention and control; procedures; water management; water pollutant; Ammonia; Bacteria; Base Sequence; Biodegradation, Environmental; Industrial Waste; Nitrites; Oxidoreductases; Petroleum; RNA, Ribosomal, 16S; Water Pollutants, Chemical; Water Purification; Bacteria (microorganisms); Nitrobacter; Nitrosomonadales; Nitrosomonas europaea; Nitrosomonas nitrosa; Nitrospira; Ammonia; Bacteria; Base Sequence; Biodegradation, Environmental; Industrial Waste; Nitrites; Oxidoreductases; Petroleum; RNA, Ribosomal, 16S; Water Pollutants, Chemical; Water Purification
Año:2010
Volumen:181
Número:1-3
Página de inicio:281
Página de fin:288
DOI: http://dx.doi.org/10.1016/j.jhazmat.2010.05.009
Título revista:Journal of Hazardous Materials
Título revista abreviado:J. Hazard. Mater.
ISSN:03043894
CODEN:JHMAD
CAS:DNA, 9007-49-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; nitrite, 14797-65-0; petroleum, 8002-05-9; phenol, 108-95-2, 3229-70-7; unspecific monooxygenase, 9012-80-0, 9037-52-9, 9038-14-6; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; Ammonia, 7664-41-7; Industrial Waste; Nitrites; Oxidoreductases, 1.-; Petroleum; RNA, Ribosomal, 16S; Water Pollutants, Chemical; ammonia monooxygenase, 1.7.3.-; Ammonia; ammonia monooxygenase; Industrial Waste; Nitrites; Oxidoreductases; Petroleum; RNA, Ribosomal, 16S; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043894_v181_n1-3_p281_Figuerola

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Citas:

---------- APA ----------
Figuerola, E.L.M. & Erijman, L. (2010) . Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification. Journal of Hazardous Materials, 181(1-3), 281-288.
http://dx.doi.org/10.1016/j.jhazmat.2010.05.009
---------- CHICAGO ----------
Figuerola, E.L.M., Erijman, L. "Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification" . Journal of Hazardous Materials 181, no. 1-3 (2010) : 281-288.
http://dx.doi.org/10.1016/j.jhazmat.2010.05.009
---------- MLA ----------
Figuerola, E.L.M., Erijman, L. "Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification" . Journal of Hazardous Materials, vol. 181, no. 1-3, 2010, pp. 281-288.
http://dx.doi.org/10.1016/j.jhazmat.2010.05.009
---------- VANCOUVER ----------
Figuerola, E.L.M., Erijman, L. Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification. J. Hazard. Mater. 2010;181(1-3):281-288.
http://dx.doi.org/10.1016/j.jhazmat.2010.05.009