Artículo
Zhou, M.-Y.; Del Carmen Vila, M.; Gomez-Sanchez, E.P.; Gomez-Sanchez, C.E. "Cloning of two alternatively spliced 21-hydroxylase CDNAs from rat adrenal" (1997) Journal of Steroid Biochemistry and Molecular Biology. 62(4):277-286
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Abstract:
Interest in extra-adrenal corticosteroid synthesis has been revived by technological advances and the quest for answers to clinical problems. The cytochrome P450 21-hydroxylase converts progesterone to deoxycorticosterone, the obligatory substrate for the production of the main adrenal steroids aldosterone, cortisol and corticosterone. The rat P450 21-hydroxylase was cloned and two constructs, 21OH-5 and 21OH-6, sequenced. The constructs are similar, except that 21OH-6 has three additional major insertions of 64, 70 and 84 bp, a 3 bp deletion, and four extra base pairs immediately before the poly-A sequence. The entire coding region of 21OH-5 has 87 and 71% homology with the mouse and human 21-hydroxylase cDNA, respectively, whereas the encoded protein has 84 and 65% homology. Reverse transcriptase-polymerase chain reaction (RT-PCR) combined with Southern blot demonstrated expression of both transcripts in the kidney, aorta, liver, cerebellum, hypothalamus and brain stem, heart and cerebrum, but not the hippocampus, in addition to the adrenal, The entire coding region of 21OH-5 and the corresponding region of 21OH-6 including the three introns were cloned into pCR3 and the plasmids transiently transfected into COS-7 cells. Only 22OH-5 was translated into active protein, converting approximately 64% of 3H-progesterone to deoxycorticosterone in 2 h.
Registro:
| Documento: | Artículo |
| Título: | Cloning of two alternatively spliced 21-hydroxylase CDNAs from rat adrenal |
| Autor: | Zhou, M.-Y.; Del Carmen Vila, M.; Gomez-Sanchez, E.P.; Gomez-Sanchez, C.E. |
| Filiación: | Division of Endocrinology, Department of Internal Medicine, University of Missouri-Columbia, Columbia, MO 65201, United States Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Harry S. Truman Mem. Vet. Hospital, University of Missouri-Columbia, Columbia, MO 65201, United States Dept. of Vet. Biomedical Sciences, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO 65201, United States |
| Palabras clave: | complementary dna; cytochrome p450; steroid 21 monooxygenase; adrenal gland; animal cell; animal tissue; aorta; article; brain; brain stem; cerebellum; cho cell; controlled study; enzyme activity; gene construct; gene expression; heart; histochemistry; hypothalamus; kidney; liver; molecular cloning; mutant; nonhuman; rat; reverse transcription polymerase chain reaction; sequence analysis; sequence homology; southern blotting; Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; COS Cells; DNA, Complementary; Molecular Sequence Data; Rats; Rats, Sprague-Dawley; Sequence Alignment; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Steroid 21-Hydroxylase; Steroids; Zona Glomerulosa; Animalia |
| Año: | 1997 |
| Volumen: | 62 |
| Número: | 4 |
| Página de inicio: | 277 |
| Página de fin: | 286 |
| DOI: | http://dx.doi.org/10.1016/S0960-0760(97)00040-X |
| Título revista: | Journal of Steroid Biochemistry and Molecular Biology |
| Título revista abreviado: | J. STEROID BIOCHEM. MOL. BIOL. |
| ISSN: | 09600760 |
| CODEN: | JSBBE |
| CAS: | DNA, Complementary; Steroid 21-Hydroxylase, EC 1.14.99.10; Steroids |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v62_n4_p277_Zhou |
Referencias:
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Citas:
---------- APA ----------
Zhou, M.-Y., Del Carmen Vila, M., Gomez-Sanchez, E.P. & Gomez-Sanchez, C.E. (1997) . Cloning of two alternatively spliced 21-hydroxylase CDNAs from rat adrenal. Journal of Steroid Biochemistry and Molecular Biology, 62(4), 277-286.http://dx.doi.org/10.1016/S0960-0760(97)00040-X
---------- CHICAGO ----------
Zhou, M.-Y., Del Carmen Vila, M., Gomez-Sanchez, E.P., Gomez-Sanchez, C.E. "Cloning of two alternatively spliced 21-hydroxylase CDNAs from rat adrenal" . Journal of Steroid Biochemistry and Molecular Biology 62, no. 4 (1997) : 277-286.http://dx.doi.org/10.1016/S0960-0760(97)00040-X
---------- MLA ----------
Zhou, M.-Y., Del Carmen Vila, M., Gomez-Sanchez, E.P., Gomez-Sanchez, C.E. "Cloning of two alternatively spliced 21-hydroxylase CDNAs from rat adrenal" . Journal of Steroid Biochemistry and Molecular Biology, vol. 62, no. 4, 1997, pp. 277-286.http://dx.doi.org/10.1016/S0960-0760(97)00040-X
---------- VANCOUVER ----------
Zhou, M.-Y., Del Carmen Vila, M., Gomez-Sanchez, E.P., Gomez-Sanchez, C.E. Cloning of two alternatively spliced 21-hydroxylase CDNAs from rat adrenal. J. STEROID BIOCHEM. MOL. BIOL. 1997;62(4):277-286.http://dx.doi.org/10.1016/S0960-0760(97)00040-X
