Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

A full-length cDNA clone (LeCDPK1) from tomato (Lycopersicon esculentum) encoding a calcium-dependent protein kinase (CDPK) was isolated by screening a cDNA library from tomato cell cultures exposed to Cladosporium fulvum elicitor preparations. The predicted amino acid sequence of the cDNA reveals a high degree of similarity with other members of the CDPK family. LeCDPK1 has a putative N-terminal myristoylation sequence and presents a possible palmitoylation site. The in vitro translated protein conserves the biochemical properties of a member of the CDPK family. In addition, CDPK activity was detected in soluble and particulate extracts of tomato leaves. Basal levels of LeCDPK1 mRNA were detected by northern-blot analysis in roots, stems, leaves, and flowers of tomato plants. The expression of LeCDPK1 was rapidly and transiently enhanced in detached tomato leaves treated with pathogen elicitors and H2O2. Moreover, when tomato greenhouse plants were subjected to mechanical wounding, a transient increase of LeCDPK1 steady-state mRNA levels was detected locally at the site of the injury and systemically in distant non-wounded leaves. The increase observed in LeCDPK1 mRNA upon wounding correlates with an increase in the amount and in the activity of a soluble CDPK detected in extracts of tomato leaves, suggesting that this kinase is part of physiological plant defense mechanisms against biotic or abiotic attacks.

Registro:

Documento: Artículo
Título:A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants
Autor:Chico, J.M.; Raíces, M.; Téllez-Iñón, M.T.; Ulloa, R.M.
Filiación:Inst. Invest. Ingen. Gen. Biol. Mol., Consejo Nac. de Invest. Cie. y Tec., Vuelta de Obligado 2490 2do piso, 1428 Buenos Aires, Argentina
Palabras clave:Amino acids; Biological membranes; Calcium; DNA; Enzyme kinetics; Physiology; Plant cell culture; Proteins; RNA; Defense mechanisms; Plants (botany); Cladosporium fulvum; Cladosporium fulvum; Embryophyta; Lycopersicon esculentum; Lycopersicon esculentum; calcium binding protein; messenger RNA; protein kinase; adaptation; amino acid sequence; article; Colletotrichum; enzymology; gene expression regulation; genetics; growth, development and aging; innate immunity; isolation and purification; metabolism; microbiology; molecular cloning; molecular genetics; Northern blotting; plant disease; plant leaf; sequence homology; signal transduction; Southern blotting; tomato; Western blotting; Adaptation, Physiological; Amino Acid Sequence; Blotting, Northern; Blotting, Southern; Blotting, Western; Calcium-Binding Proteins; Cloning, Molecular; Colletotrichum; Gene Expression Regulation, Enzymologic; Immunity, Natural; Lycopersicon esculentum; Molecular Sequence Data; Plant Diseases; Plant Leaves; Protein Kinases; RNA, Messenger; Sequence Homology, Amino Acid; Signal Transduction
Año:2002
Volumen:128
Número:1
Página de inicio:256
Página de fin:270
DOI: http://dx.doi.org/10.1104/pp.010649
Título revista:Plant Physiology
Título revista abreviado:Plant Physiol.
ISSN:00320889
CODEN:PLPHA
CAS:protein kinase, 9026-43-1; Calcium-Binding Proteins; Protein Kinases, EC 2.7.1.37; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v128_n1_p256_Chico

Referencias:

  • Alvarez, M.E., Pennell, R.I., Meijer, P.J., Ishikawa, A., Dixon, R.A., Lamb, C., Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity (1998) Cell, 92, pp. 773-784
  • Alwine, J.C., Kemp, D.J., Stark, G.R., Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes (1977) Proc Natl Acad Sci USA, 74, pp. 5350-5354
  • Bachmann, M., Shiraishi, N., Campbell, W.H., Yoo, B.C., Harmon, A.C., Huber, S.C., Identification of Ser-543 as the major regulatory phosphorylation site in spinach leaf nitrate reductase (1996) Plant Cell, 8, pp. 505-517
  • Bell, E., Mullet, J.E., Characterization of an Arabidopsis lipoxygenase gene responsive to methyl jasmonate and wounding (1993) Plant Physiol, 103, pp. 1133-1137
  • Bergey, D.R., Orozco-Cárdenas, M., De Moura, D.S., Ryan, C.A., A wound- and systemin-inducible polygalacturonase in tomato (1999) Proc Natl Acad Sci USA, 96, pp. 1756-1760
  • Blume, B., Nurnberger, T., Nass, N., Scheel, D., Receptor-mediated increase in cytoplasmic free calcium required for activation of pathogen defense in parsley (2000) Plant Cell, 12, pp. 1425-1440
  • Bonner, W.M., Laskey, R.A., A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels (1974) Eur J Biochem, 1, pp. 83-88
  • Bowles, D.J., Defense-related proteins in higher plants (1990) Annu Rev Biochem, 59, pp. 873-907
  • Bowles, D.J., Signal transduction in the wound response of tomato plants (1998) Philos Trans R Soc Lond B Biol Sci, 353, pp. 1495-1510
  • Carrera, E., Prat, S., Expression of the Arabidopsis abi1-1 mutant allele inhibits -proteinase inhibitor wound-induction in tomato (1998) Plant J, 15, pp. 765-771
  • Church, G.M., Gilbert, W., Genomic sequencing (1984) Proc Natl Acad Sci USA, 81, pp. 1991-1995
  • Conconi, A., Smerdon, M.J., Howe, G.A., Ryan, C.A., The octadecanoid signalling pathway in plants mediates a response to ultraviolet radiation (1996) Nature, 383, pp. 826-829
  • Darvill, A., Albersheim, P., Phytoalexins and their elicitors: A defense against insects and pathogens (1984) Annu Rev Plant Physiol, 53, pp. 243-275
  • Desikan, R., Neill, S.J., Hancock, J.T., Hydrogen peroxide-induced gene expression in Arabidopsis thaliana (2000) Free Radic Biol Med, 28, pp. 773-778
  • Ellard-Ivey, M., Hopkins, R.B., White, T.J., Lomax, T.L., Cloning, expression and N-terminal myristoylation of CpCPK1, a calcium-dependent protein kinase from zucchini (Cucurbita pepo L.) (1999) Plant Mol Biol, 39, pp. 199-208
  • Farmer, E.E., Ryan, C.A., Interplant communication: Airborne methyl jasmonate induces synthesis of proteinase inhibitor in plant leaves (1990) Proc Natl Acad Sci USA, 96, pp. 12947-12952
  • Farmer, E.E., Ryan, C.A., Octadenoic precursors of jasmonic acid activate the synthesis of wound-inducible proteinase inhibitors (1992) Plant Cell, 4, pp. 129-134
  • Felix, G., Regenass, M., Boller, T., Specific perception of subnanomolar concentrations of chitin fragments by tomato cells: Induction of extracellular alkalinization, changes in protein phosphorylation, and establishment of a refractory state (1993) Plant J, 4, pp. 307-316
  • Flor, H.H., Current status of the gene-for-gene concept (1971) Annu Rev Phytopathol, 9, pp. 275-296
  • Green, T.R., Ryan, C.A., Wound-induced proteinase inhibitors in plant leaves: A possible defense against insects (1972) Science, 175, pp. 776-777
  • Halterman, D.A., Martin, G.B., Signal recognition and transduction involved in plant disease resistance (1997) Essays Biochem, 32, pp. 87-99
  • Hanaoka, H., Fukuda, A., Sasaki, T., Nemoto, K., Fujiwara, T., Hayashi, H., CDPK in rice phloem sap (1999) Interactions and Intersections in Plant Signaling Pathways-Keystone Symposia, p. 57
  • Heitz, T., Bergey, D.R., Ryan, C.A., A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding, systemin, and methyl jasmonate (1997) Plant Physiol, 114, pp. 1085-1093
  • Herde, O., Fuss, H., Peña-Cortés, H., Fisahn, J., Proteinase inhibitor II gene expression induced by electrical stimulation and control of photosynthetic activity in tomato plants (1995) Plant Cell Physiol, 36, pp. 737-742
  • Hildmann, T., Ebneth, M., Peña-Cortés, H., Sánchez-Serrano, J.J., Willmitzer, L., Prat, S., General roles of abscisic and jasmonic acid in gene activation as a result of mechanical wounding (1992) Plant Cell, 4, pp. 1157-1170
  • Hrabak, E.M., Dickmann, L.J., Satterlee, J.S., Sussman, M.R., Characterization of eight new members of the calmodulin-like domain protein kinase gene family from Arabidopsis thaliana (1996) Plant Mol Biol, 31, pp. 405-412
  • Korsmeyer, S.J., Yin, X.M., Oltvai, Z.N., Veis-Novack, D.J., Linette, G.P., Reactive oxygen species and the regulation of cell death by the Bcl-2 gene family (1995) Biochem Biophys Acta, 1271, pp. 63-66
  • Kovtun, Y., Chiu, W.L., Tena, G., Sheen, J., Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants (2000) Proc Natl Acad Sci USA, 97, pp. 2940-2945
  • Lazarovits, G., Bhullar, B.S., Sugiyama, H.J., Higgins, V.J., Purification and partial characterization of a glycoprotein toxin produced by Cladosporium fulvum (1979) Phytopathology, 69, pp. 1062-1068
  • León, J., Rojo, E., Sánchez-Serrano, J.J., Wound signalling in plants (2001) J Exp Bot, 52, pp. 1-9
  • Levine, A., Tenhaken, R., Dixon, R., Lamb, C., H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response (1994) Cell, 79, pp. 583-593
  • Ligterink, W., Kroj, T., Zur Nieden, U., Hirt, H., Scheel, D., Receptor-mediated activation of a MAP kinase in pathogen defense of plants (1997) Science, 276, pp. 2054-2057
  • MacIntosh, G.C., Ulloa, R.M., Raíces, M., Téllez-Iñón, M.T., Changes in calcium-dependent protein kinase activity during in vitro tuberization in potato (1996) Plant Physiol, 112, pp. 1541-1550
  • Martin, G.B., Brommonschenkel, S.H., Chunwongse, J., Frary, A., Ganal, M.W., Spivey, R., Wu, T., Tanksley, S.D., MAP-based cloning of a protein kinase gene conferring disease resistance in tomato (1993) Science, 262, pp. 1432-1436
  • Martín, M.L., Busconi, L., Membrane localization of a rice calcium-dependent protein kinase (CDPK) is mediated by myristoylation and palmitoylation (2000) Plant J, 24, pp. 429-435
  • Menke, F.L., Parchmann, S., Mueller, M.J., Kijne, J.W., Memelink, J., Involvement of the octadecanoid pathway and protein phosphorylation in fungal elicitor-induced expression of terpenoid indole alkaloid biosynthetic genes in Catharanthus roseus (1999) Plant Physiol, 119, pp. 1289-1296
  • Moura, D.S., Bergey, D.R., Ryan, C.A., Characterization and localization of a wound-inducible type I serinecarboxypeptidase from leaves of tomato plants (Lycopersicon esculentum Mill.) (2001) Planta, 212, pp. 222-230
  • Murray, M.G., Thompson, W.F., Rapid isolation of high molecular weight plant DNA (1980) Nucleic Acids Res, 8, pp. 4321-4325
  • Orozco-Cárdenas, M., Narvaez-Vasquez, J., Ryan, C.A., Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate (2001) Plant Cell, 13, pp. 179-191
  • Orozco-Cárdenas, M., Ryan, C.A., Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway (1999) Proc Natl Acad Sci USA, 96, pp. 6553-6557
  • Parchmann, S., Gundlach, H., Mueller, M.J., Induction of 12-oxo-phytodienoic acid in wounded plants and elicited plant cell cultures (1997) Plant Physiol, 115, pp. 1057-1064
  • Patharkar, O.R., Cushman, J.C., A stress-induced calcium-dependent protein kinase from Mesembryanthemum crystallinum phosphorylates a two-component pseudo-response regulator (2000) Plant J, 24, pp. 679-691
  • Pearce, G., Strydom, D., Jonhnson, S., Ryan, C.A., A polypeptide from tomato leaves induces wound-inducible proteinase inhibitor proteins (1991) Science, 253, pp. 895-898
  • Peña-Cortés, H., Albrecht, T., Prat, S., Weiler, E.W., Willmitzer, L., Aspirin prevents wound-induced gene expression in tomato leaves by blocking jasmonic acid biosynthesis (1993) Planta, 191, pp. 123-128
  • Peña-Cortés, H., Sánchez-Serrano, J.J., Mertens, R., Willmitzer, L., Abscisic acid is involved in the wound-induced expression of th proteinase inhibitor II gene in potato and tomato (1989) Proc Natl Acad Sci USA, 86, pp. 9851-9855
  • Raíces, M., Chico, J.M., Téllez-Iñón, M.T., Ulloa, R.M., Molecular characterization of StCDPK1, a calcium-dependent protein kinase from Solanum tuberosum that is induced at the onset of tuber development (2001) Plant Mol Biol, 46, pp. 591-601
  • Roberts, D.M., Harmon, A.C., Calcium-modulated proteins: Targets of intracellular calcium signals in higher plants (1992) Annu Rev Plant Physiol Plant Mol Biol, 43, pp. 375-414
  • Rojo, E., Titarenko, E., León, J., Berger, S., Vancanneyt, G., Sánchez-Serrano, J.J., Reversible protein phosphorylation regulates jasmonic acid-dependent and independent wound signal transduction pathways in Arabidopsis thaliana (1998) Plant J, 13, pp. 153-165
  • Romeis, T., Piedras, P., Jones, J.D., Resistance gene-dependent activation of a calcium-dependent protein kinase in the plant defense response (2000) Plant Cell, 12, pp. 803-816
  • Romeis, T., Piedras, P., Zang, S., Klessig, D.F., Hirt, H., Jones, J.D., Avr9- and Cf-9-dependent activation of MAP kinase in tobacco cell cultures and leaves: Convergence of resistance genes, elicitor, wound and salicylate responses (1999) Plant Cell, 11, pp. 273-287
  • Ruiz-Medrano, R., Xoconostle-Cázares, B., Lucas, W.J., Phloem long-distance transport of CmNACP mRNA: Implications for supracellular regulation in plants (1999) Development, 126, pp. 4405-4419
  • Rushton, P.J., Somssich, I.E., Transcriptional control of plant genes responsive to pathogens (1998) Curr Opin Plant Biol, 1, pp. 311-315
  • Ryan, C.A., Oligosaccharide signalling in plants (1987) Annu Rev Cell Biol, 3, pp. 295-317
  • Ryan, C.A., Oligosaccharides as recognition signals for the expression of defensive genes in plants (1988) Biochemistry, 27, pp. 8879-8883
  • Ryan, C.A., Protease inhibitors in plants: Genes for improving defenses against insects and pathogens (1990) Annu Rev Phytopathol, 28, pp. 425-449
  • Saravitz, D.M., Siedow, J.N., The differential expression of wound-inducible lipoxygenase genes in soybean leaves (1996) Plant Physiol, 110, pp. 287-299
  • Sasaki, T., Chino, M., Hayashi, H., Fujiwara, T., Detection of several mRNA species in rice phloem sap (1998) Plant Cell Physiol, 39, pp. 895-897
  • Schaller, A., Oecking, C., Modulation of plasma membrane H+-ATPase activity differentially activates wound and pathogen defense responses in tomato plants (1999) Plant Cell, 11, pp. 263-272
  • Scheel, D., Resistance response physiology and signal transduction (1998) Curr Opin Plant Biol, 1, pp. 305-310
  • Song, W.Y., Wang, G.L., Chen, L.L., Kim, H.S., Pi, L.Y., Holsten, T., Gardner, J., Ronald, P., A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21 (1995) Science, 270, pp. 1804-1806
  • Sopory, S.K., Munshi, M., Protein kinases and phosphatases and their role in cellular signaling in plants (1998) Crit Rev Plant Sci, 17, pp. 245-318
  • Staskawicz, B.J., Ausubel, F.M., Baker, B.J., Ellis, J.G., Jones, J.D., Molecular genetics of plant disease resistance (1995) Science, 268, pp. 661-667
  • Wildon, D.C., Thain, J.F., Minchin, P.E.H., Gubb, I.R., Reilly, A.J., Skipper, Y.D., Doherty, H.M., Bowles, D.J., Electrical signalling and systemic proteinase inhibitor induction in the wounded plant (1992) Nature, 360, pp. 62-65
  • Xoconostle-Cázares, B., Xiang, Y., Ruiz-Medrano, R., Wang, H.L., Monzer, J., Yoo, B.C., McFarland, K.C., Lucas, W.J., Plant paralog to viral movement protein that potentiates transport of mRNA into the phloem (1999) Science, 283, pp. 94-98
  • Yang, Y., Shah, J., Klessig, D.F., Signal perception and transduction in plant defense responses (1997) Genes Dev, 11, pp. 1621-1639
  • Zhang, S., Du, H., Klessig, D.F., Activation of the tobacco SIP kinase by both a cell wall-derived carbohydrate elicitor and purified proteinaceous elicitins from Phytophthora spp. (1998) Plant Cell, 10, pp. 435-449

Citas:

---------- APA ----------
Chico, J.M., Raíces, M., Téllez-Iñón, M.T. & Ulloa, R.M. (2002) . A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants. Plant Physiology, 128(1), 256-270.
http://dx.doi.org/10.1104/pp.010649
---------- CHICAGO ----------
Chico, J.M., Raíces, M., Téllez-Iñón, M.T., Ulloa, R.M. "A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants" . Plant Physiology 128, no. 1 (2002) : 256-270.
http://dx.doi.org/10.1104/pp.010649
---------- MLA ----------
Chico, J.M., Raíces, M., Téllez-Iñón, M.T., Ulloa, R.M. "A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants" . Plant Physiology, vol. 128, no. 1, 2002, pp. 256-270.
http://dx.doi.org/10.1104/pp.010649
---------- VANCOUVER ----------
Chico, J.M., Raíces, M., Téllez-Iñón, M.T., Ulloa, R.M. A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants. Plant Physiol. 2002;128(1):256-270.
http://dx.doi.org/10.1104/pp.010649