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Abstract:

Some strains of Bacillus sphaericus are entomopathogenic to mosquito larvae, which transmit diseases, such as filariasis and malaria, affecting millions of people worldwide. This species is unable to use hexoses and pentoses as unique carbon sources, which was proposed to be due to the lack of glycolytic enzymes, such as 6-phosphofructokinase (PFK). In this study, PFK activity was detected and the pfk gene was cloned and sequenced. Furthermore, this gene was shown to be present in strains belonging to all the homology groups of this heterogeneous species, in which PFK activity was also detected. A careful sequence analysis revealed the conservation of different catalytic and regulatory residues, as well as the enzyme's phylogenetic affiliation with the family of allosteric ATP-PFK enzymes.

Registro:

Documento: Artículo
Título:Existence of a true phosphofructokinase in Bacillus sphaericus: Cloning and sequencing of the pfk gene
Autor:Alice, A.F.; Pérez-Martínez, G.; Sáchez-Rivas, C.
Filiación:Departamento de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires (1428), Argentina
Departamento de Biotecnología, Inst. Agroquimica Tecnol. Alimentos, CSIC, Paterna, Valencia, Spain
Palabras clave:Diseases; Enzymes; Genes; Genetic engineering; Mosquito larvae; Microbiology; 6 phosphofructokinase; glycolytic enzyme; hexose; pentose; bacterium; article; Bacillus sphaericus; bacterial gene; bacterial strain; catalysis; disease transmission; entomopathogeny; enzyme activity; filariasis; gene sequence; malaria; molecular cloning; nonhuman; nucleotide sequence; pfk gene; phylogeny; sequence analysis; Amino Acid Sequence; Bacillus; Base Sequence; Cloning, Molecular; Genetic Complementation Test; Molecular Sequence Data; Phosphofructokinases; Polymerase Chain Reaction; Bacillus sphaericus; Bacteria (microorganisms); Posibacteria; Prokaryota
Año:2002
Volumen:68
Número:12
Página de inicio:6410
Página de fin:6415
DOI: http://dx.doi.org/10.1128/AEM.68.12.6410-6415.2002
Handle:http://hdl.handle.net/20.500.12110/paper_00992240_v68_n12_p6410_Alice
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
CAS:6 phosphofructokinase, 9001-80-3; hexose, 93780-23-5; pentose, 53106-52-8; Phosphofructokinases, EC 2.7.1 -
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00992240_v68_n12_p6410_Alice.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v68_n12_p6410_Alice

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Citas:

---------- APA ----------
Alice, A.F., Pérez-Martínez, G. & Sáchez-Rivas, C. (2002) . Existence of a true phosphofructokinase in Bacillus sphaericus: Cloning and sequencing of the pfk gene. Applied and Environmental Microbiology, 68(12), 6410-6415.
http://dx.doi.org/10.1128/AEM.68.12.6410-6415.2002
---------- CHICAGO ----------
Alice, A.F., Pérez-Martínez, G., Sáchez-Rivas, C. "Existence of a true phosphofructokinase in Bacillus sphaericus: Cloning and sequencing of the pfk gene" . Applied and Environmental Microbiology 68, no. 12 (2002) : 6410-6415.
http://dx.doi.org/10.1128/AEM.68.12.6410-6415.2002
---------- MLA ----------
Alice, A.F., Pérez-Martínez, G., Sáchez-Rivas, C. "Existence of a true phosphofructokinase in Bacillus sphaericus: Cloning and sequencing of the pfk gene" . Applied and Environmental Microbiology, vol. 68, no. 12, 2002, pp. 6410-6415.
http://dx.doi.org/10.1128/AEM.68.12.6410-6415.2002
---------- VANCOUVER ----------
Alice, A.F., Pérez-Martínez, G., Sáchez-Rivas, C. Existence of a true phosphofructokinase in Bacillus sphaericus: Cloning and sequencing of the pfk gene. Appl. Environ. Microbiol. 2002;68(12):6410-6415.
http://dx.doi.org/10.1128/AEM.68.12.6410-6415.2002