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Solari, C.; Echegaray, C.V.; Cosentino, M.S.; Petrone, M.V.; Waisman, A.; Luzzani, C.; Francia, M.; Villodre, E.; Lenz, G.; Miriuka, S.; Barañao, L.; Guberman, A. "Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors" (2015) PLoS ONE. 10(12)
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Abstract:

Pluripotent stem cells possess complex systems that protect them from oxidative stress and ensure genomic stability, vital for their role in development. Even though it has been reported that antioxidant activity diminishes along stem cell differentiation, little is known about the transcriptional regulation of the involved genes. The reported modulation of some of these genes led us to hypothesize that some of them could be regulated by the transcription factors critical for self-renewal and pluripotency in embryonic stem cells (ESCs) and in induced pluripotent stem cells (iPSCs). In this work, we studied the expression profile of multiple genes involved in antioxidant defense systems in both ESCs and iPSCs. We found that Manganese superoxide dismutase gene (Mn-Sod/Sod2) was repressed during diverse differentiation protocols showing an expression pattern similar to Nanog gene. Moreover, Sod2 promoter activity was induced by Oct4 and Nanog when we performed a transactivation assay using two different reporter constructions. Finally, we studied Sod2 gene regulation by modulating the expression of Oct4 and Nanog in ESCs by shRNAs and found that downregulation of any of them reduced Sod2 expression. Our results indicate that pluripotency transcription factors positively modulate Sod2 gene transcription. © 2015 Solari et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Registro:

Documento: Artículo
Título:Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors
Autor:Solari, C.; Echegaray, C.V.; Cosentino, M.S.; Petrone, M.V.; Waisman, A.; Luzzani, C.; Francia, M.; Villodre, E.; Lenz, G.; Miriuka, S.; Barañao, L.; Guberman, A.
Filiación:Laboratorio de Regulación Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Química Biológica (IQUIBICEN), UBA-CONICET, Buenos Aires, Argentina
Laboratório de Sinalização Celular, Departamento de Biofísica, Universidade Federal Do Rio Grande Do sul, Porto Alegre, Brazil
Laboratorio de Investigación Aplicada A Las Neurociencias (LIAN), Fundación para la Lucha Contra Las Enfermedades Neurológicas de la Infancia (FLENI), Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
LIAN, FLENI, Buenos Aires, Argentina
Palabras clave:manganese superoxide dismutase; messenger RNA; octamer transcription factor 4; short hairpin RNA; transcription factor NANOG; transcription factor Sox2; homeodomain protein; manganese superoxide dismutase; Nanog protein, mouse; octamer transcription factor 4; Pou5f1 protein, mouse; small interfering RNA; superoxide dismutase; transcription factor NANOG; animal cell; Article; binding site; cell differentiation; cell renewal; cellular parameters; computer model; controlled study; down regulation; embryo; embryonic stem cell; gene expression profiling; gene expression regulation; gene identification; gene repression; gene sequence; molecular cloning; molecular dynamics; mouse; Nanog gene; nonhuman; Oct4 gene; oxidative stress; pluripotency; pluripotent stem cell; promoter region; Sod2 gene; transactivation; transcription initiation site; transcription regulation; animal; cell line; cytology; genetics; induced pluripotent stem cell; metabolism; physiology; Animals; Cell Differentiation; Cell Line; Embryonic Stem Cells; Gene Expression Regulation; Gene Expression Regulation, Enzymologic; Homeodomain Proteins; Induced Pluripotent Stem Cells; Mice; Nanog Homeobox Protein; Octamer Transcription Factor-3; Promoter Regions, Genetic; RNA, Small Interfering; Superoxide Dismutase
Año:2015
Volumen:10
Número:12
DOI: http://dx.doi.org/10.1371/journal.pone.0144336
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Homeodomain Proteins; Nanog Homeobox Protein; Nanog protein, mouse; Octamer Transcription Factor-3; Pou5f1 protein, mouse; RNA, Small Interfering; Superoxide Dismutase; superoxide dismutase 2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v10_n12_p_Solari

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Citas:

---------- APA ----------
Solari, C., Echegaray, C.V., Cosentino, M.S., Petrone, M.V., Waisman, A., Luzzani, C., Francia, M.,..., Guberman, A. (2015) . Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors. PLoS ONE, 10(12).
http://dx.doi.org/10.1371/journal.pone.0144336
---------- CHICAGO ----------
Solari, C., Echegaray, C.V., Cosentino, M.S., Petrone, M.V., Waisman, A., Luzzani, C., et al. "Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors" . PLoS ONE 10, no. 12 (2015).
http://dx.doi.org/10.1371/journal.pone.0144336
---------- MLA ----------
Solari, C., Echegaray, C.V., Cosentino, M.S., Petrone, M.V., Waisman, A., Luzzani, C., et al. "Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors" . PLoS ONE, vol. 10, no. 12, 2015.
http://dx.doi.org/10.1371/journal.pone.0144336
---------- VANCOUVER ----------
Solari, C., Echegaray, C.V., Cosentino, M.S., Petrone, M.V., Waisman, A., Luzzani, C., et al. Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors. PLoS ONE. 2015;10(12).
http://dx.doi.org/10.1371/journal.pone.0144336