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Abstract:

The variable genes of monoclonal antibody (mAb) B10, specific for the C-terminal region of the eukaryotic ribosomal P protein, have been cloned as a single-chain Fv fragment (scFv) and expressed in Escherichia coli. The primary sequence of the variable regions of the B10 antibody, together with a detailed characterization of the reactive residues of the antigen, allowed the construction of a model of the paratope-epitope interaction, giving a first insight into the binding mechanisms of anti-P autoantibodies to their target peptides. The mAb and scFv could be useful for extensive P protein detection since both recognize the highly conserved motif DDxGF. © 2003 Elsevier Science (USA). All rights reserved.

Registro:

Documento: Artículo
Título:Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins
Autor:López Bergami, P.; Mateos, P.; Hoebeke, J.; Levin, M.J.; Baldi, A.
Filiación:Inst. de Biol. y Med. Exp. (IBYME), Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Lab. Biol. Molec. Enfermedad Chagas, Inst. Invest. Ing. Genet. Biol. M., Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
UPR 9021 Immunol. Chim. T., 67084 Strasbourg Cedex, France
Palabras clave:Autoantibody; Paratope-epitope modelling; Ribosomal P protein; Single-chain Fv fragment; Trypanosoma cruzi; autoantibody; epitope; monoclonal antibody; ribosome protein; antibody combining site; article; binding affinity; carboxy terminal sequence; Escherichia coli; eukaryote; molecular biology; molecular cloning; molecular interaction; nonhuman; nucleotide sequence; priority journal; protein analysis; protein expression; sequence analysis; Escherichia coli; Eukaryota; Trypanosoma; Trypanosoma cruzi
Año:2003
Volumen:301
Número:4
Página de inicio:819
Página de fin:824
DOI: http://dx.doi.org/10.1016/S0006-291X(02)03074-7
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v301_n4_p819_LopezBergami

Referencias:

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  • Elkon, K.B., Parnassa, A.P., Foster, C.L., Antibodies to ribosomal P proteins of Trypanosoma cruzi in Chagas disease possess functional autoreactivity with heart tissue and differ from anti-P autoantibodies in lupus (1985) J. Exp. Med., 162, pp. 459-471
  • Kaplan, D., Ferrari, I., Lopez Bergami, P., Mahler, E., Levitus, G., Chiale, P., Hoebeke, J., Levin, M., Antibodies to ribosomal P proteins of Trypanosoma cruzi in Chagas disease possess functional autoreactivity with heart tissue and differ from anti-P autoantibodies in lupus (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 10301-10306
  • Chiale, P.A., Ferrari, I., Mahler, E., Vallazza, M.A., Elizari, M.V., Rosenbaum, M.B., Levin, M.J., Differential profile and biochemical effects of antiautonomic membrane receptor antibodies in ventricular arrhythmias and sinus node dysfunction (2001) Circulation, 103, pp. 1765-1771
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Citas:

---------- APA ----------
López Bergami, P., Mateos, P., Hoebeke, J., Levin, M.J. & Baldi, A. (2003) . Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins. Biochemical and Biophysical Research Communications, 301(4), 819-824.
http://dx.doi.org/10.1016/S0006-291X(02)03074-7
---------- CHICAGO ----------
López Bergami, P., Mateos, P., Hoebeke, J., Levin, M.J., Baldi, A. "Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins" . Biochemical and Biophysical Research Communications 301, no. 4 (2003) : 819-824.
http://dx.doi.org/10.1016/S0006-291X(02)03074-7
---------- MLA ----------
López Bergami, P., Mateos, P., Hoebeke, J., Levin, M.J., Baldi, A. "Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins" . Biochemical and Biophysical Research Communications, vol. 301, no. 4, 2003, pp. 819-824.
http://dx.doi.org/10.1016/S0006-291X(02)03074-7
---------- VANCOUVER ----------
López Bergami, P., Mateos, P., Hoebeke, J., Levin, M.J., Baldi, A. Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins. Biochem. Biophys. Res. Commun. 2003;301(4):819-824.
http://dx.doi.org/10.1016/S0006-291X(02)03074-7