Abstract:
The variable genes of monoclonal antibody (mAb) B10, specific for the C-terminal region of the eukaryotic ribosomal P protein, have been cloned as a single-chain Fv fragment (scFv) and expressed in Escherichia coli. The primary sequence of the variable regions of the B10 antibody, together with a detailed characterization of the reactive residues of the antigen, allowed the construction of a model of the paratope-epitope interaction, giving a first insight into the binding mechanisms of anti-P autoantibodies to their target peptides. The mAb and scFv could be useful for extensive P protein detection since both recognize the highly conserved motif DDxGF. © 2003 Elsevier Science (USA). All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins |
Autor: | López Bergami, P.; Mateos, P.; Hoebeke, J.; Levin, M.J.; Baldi, A. |
Filiación: | Inst. de Biol. y Med. Exp. (IBYME), Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina Lab. Biol. Molec. Enfermedad Chagas, Inst. Invest. Ing. Genet. Biol. M., Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina UPR 9021 Immunol. Chim. T., 67084 Strasbourg Cedex, France
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Palabras clave: | Autoantibody; Paratope-epitope modelling; Ribosomal P protein; Single-chain Fv fragment; Trypanosoma cruzi; autoantibody; epitope; monoclonal antibody; ribosome protein; antibody combining site; article; binding affinity; carboxy terminal sequence; Escherichia coli; eukaryote; molecular biology; molecular cloning; molecular interaction; nonhuman; nucleotide sequence; priority journal; protein analysis; protein expression; sequence analysis; Escherichia coli; Eukaryota; Trypanosoma; Trypanosoma cruzi |
Año: | 2003
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Volumen: | 301
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Número: | 4
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Página de inicio: | 819
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Página de fin: | 824
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DOI: |
http://dx.doi.org/10.1016/S0006-291X(02)03074-7 |
Título revista: | Biochemical and Biophysical Research Communications
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Título revista abreviado: | Biochem. Biophys. Res. Commun.
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ISSN: | 0006291X
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CODEN: | BBRCA
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v301_n4_p819_LopezBergami |
Referencias:
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- Elkon, K.B., Parnassa, A.P., Foster, C.L., Antibodies to ribosomal P proteins of Trypanosoma cruzi in Chagas disease possess functional autoreactivity with heart tissue and differ from anti-P autoantibodies in lupus (1985) J. Exp. Med., 162, pp. 459-471
- Kaplan, D., Ferrari, I., Lopez Bergami, P., Mahler, E., Levitus, G., Chiale, P., Hoebeke, J., Levin, M., Antibodies to ribosomal P proteins of Trypanosoma cruzi in Chagas disease possess functional autoreactivity with heart tissue and differ from anti-P autoantibodies in lupus (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 10301-10306
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Citas:
---------- APA ----------
López Bergami, P., Mateos, P., Hoebeke, J., Levin, M.J. & Baldi, A.
(2003)
. Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins. Biochemical and Biophysical Research Communications, 301(4), 819-824.
http://dx.doi.org/10.1016/S0006-291X(02)03074-7---------- CHICAGO ----------
López Bergami, P., Mateos, P., Hoebeke, J., Levin, M.J., Baldi, A.
"Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins"
. Biochemical and Biophysical Research Communications 301, no. 4
(2003) : 819-824.
http://dx.doi.org/10.1016/S0006-291X(02)03074-7---------- MLA ----------
López Bergami, P., Mateos, P., Hoebeke, J., Levin, M.J., Baldi, A.
"Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins"
. Biochemical and Biophysical Research Communications, vol. 301, no. 4, 2003, pp. 819-824.
http://dx.doi.org/10.1016/S0006-291X(02)03074-7---------- VANCOUVER ----------
López Bergami, P., Mateos, P., Hoebeke, J., Levin, M.J., Baldi, A. Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins. Biochem. Biophys. Res. Commun. 2003;301(4):819-824.
http://dx.doi.org/10.1016/S0006-291X(02)03074-7