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Fructans are fructose polymers synthesized in a wide range of species such as bacteria, fungi and plants. Fructans are synthesized by fructosyltransferases (FTs) and depolymerized by fructan exohydrolases (FEHs). Bromus pictus is a graminean decaploid species from the Patagonian region of Argentina, which accumulates large amounts of fructans even at temperate temperatures. The first gene isolated from B. pictus fructan metabolism was a putative sucrose:fructan 6-fructosyltransferase (6-SFT). Here, a complete cDNA of the first fructan exohydrolase (FEH) from B. pictus (Bp1-FEHa) was isolated using RT-PCR strategies. The Bp1-FEHa encoding gene is present as a single copy in B. pictus genome. Functional characterization in Pichia pastoris confirmed Bp1-FEHa is a fructan exohydrolase with predominant activity towards β-(2-1) linkages. Its expression was analyzed in different leaf sections, showing the highest expression levels in the second section of the sheath and the tip of the blade. Bp1-FEHa expression was studied along with FEH and FT activities and fructan accumulation profile in response to chilling conditions during a 7-day time course experiment. Bp1-FEHa expression and FEH activity followed a similar pattern in response to low temperatures, especially in basal sections of the sheaths. In these sections the FEH and FT activities were particularly high and they were significantly correlated to fructan accumulation profile, along with cold treatment. © 2009 Springer-Verlag.


Documento: Artículo
Título:Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in the cold tolerant Patagonian species Bromus pictus
Autor:Del Viso, F.; Puebla, A.F.; Hopp, H.E.; Heinz, R.A.
Filiación:Instituto de Biotecnología, CICVyA, INTA, Hurlingham, Buenos Aires 1686, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Bromus pictus; Fructan; Fructan exohydrolase; Low temperature; Patagonia; fructan; fructan beta fructosidase; fructan beta-fructosidase; glycosidase; glycosyltransferase; inulosucrase; recombinant protein; adaptation; amino acid sequence; article; Bromus; chemistry; cold; enzymology; gene expression profiling; gene expression regulation; genetics; metabolism; molecular cloning; molecular genetics; nucleotide sequence; phylogeny; Pichia; plant gene; plant leaf; sequence alignment; Southern blotting; Adaptation, Physiological; Amino Acid Sequence; Blotting, Southern; Bromus; Cloning, Molecular; Cold Temperature; Fructans; Gene Expression Profiling; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes, Plant; Glycoside Hydrolases; Hexosyltransferases; Molecular Sequence Data; Phylogeny; Pichia; Plant Leaves; Recombinant Proteins; Sequence Alignment; Bacteria (microorganisms); Bromus; Bromus pictus; Fungi; Graminea; Pichia pastoris
Página de inicio:13
Página de fin:25
Título revista:Planta
Título revista abreviado:Planta
CAS:fructan, 9037-90-5; glycosidase, 9032-92-2; glycosyltransferase, 9033-07-2; Fructans; Glycoside Hydrolases, 3.2.1.-; Hexosyltransferases, 2.4.1.-; Recombinant Proteins; fructan beta-fructosidase,; inulosucrase,


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---------- APA ----------
Del Viso, F., Puebla, A.F., Hopp, H.E. & Heinz, R.A. (2009) . Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in the cold tolerant Patagonian species Bromus pictus. Planta, 231(1), 13-25.
---------- CHICAGO ----------
Del Viso, F., Puebla, A.F., Hopp, H.E., Heinz, R.A. "Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in the cold tolerant Patagonian species Bromus pictus" . Planta 231, no. 1 (2009) : 13-25.
---------- MLA ----------
Del Viso, F., Puebla, A.F., Hopp, H.E., Heinz, R.A. "Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in the cold tolerant Patagonian species Bromus pictus" . Planta, vol. 231, no. 1, 2009, pp. 13-25.
---------- VANCOUVER ----------
Del Viso, F., Puebla, A.F., Hopp, H.E., Heinz, R.A. Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in the cold tolerant Patagonian species Bromus pictus. Planta. 2009;231(1):13-25.