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Buscaglia, C.A.; Pollevick, G.D.; Veloso, C.; Lorca, M.; Frasch, A.C.C.; Sánchez, D.O. "A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi" (1996) Biochimica et Biophysica Acta - Gene Structure and Expression. 1309(1-2):53-57
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Abstract:

A full-length DNA clone encoding a putative pyruvate dehydrogenase α subunit (E1α) gene was isolated from a Trypanosoma cruzi (RA strain) DNA library. Sequencing of this clone revealed it to encode a 378 amino acid protein (M(r) 42774) with high sequence similarity to E1α obtained from different sources. The highest score is obtained with human E1α: 43.3% similarity. Southern blot analysis is consistent with the existence of a single copy of this putative T. cruzi E1α gene per haploid genome in different parasite strains. Expression of this gene was demonstrated by Northern blot analysis and its trans-splicing acceptor site was identified by Polymerase Chain Reaction-mediated amplification of its cDNA.

Registro:

Documento: Artículo
Título:A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi
Autor:Buscaglia, C.A.; Pollevick, G.D.; Veloso, C.; Lorca, M.; Frasch, A.C.C.; Sánchez, D.O.
Filiación:Inst. de Invest. Bioquimicas 'F., Antonio Machado 151, (1405) Buenos Aires, Argentina
Fac. de Cie. Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Unidad de Parasitología, Facultad de Medicina, Universidad de Chile, Las Palmeras 299, (QN) Santiago, Chile
Palabras clave:(Trypanosoma cruzi); Pyruvate dehydrogenase α subunit; Trans-splicing; pyruvate dehydrogenase; amino acid sequence; article; dna library; enzyme subunit; gene isolation; human; molecular cloning; nonhuman; nucleotide sequence; priority journal; sequence homology; trypanosoma cruzi; yeast; Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA, Protozoan; Gene Dosage; Genes, Protozoan; Humans; Molecular Sequence Data; Pyruvate Dehydrogenase Complex; RNA, Messenger; RNA, Protozoan; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Trypanosoma cruzi; Trypanosoma; Trypanosoma cruzi
Año:1996
Volumen:1309
Número:1-2
Página de inicio:53
Página de fin:57
DOI: http://dx.doi.org/10.1016/S0167-4781(96)00140-6
Título revista:Biochimica et Biophysica Acta - Gene Structure and Expression
Título revista abreviado:BIOCHIM. BIOPHYS. ACTA GENE STRUCT. EXPR.
ISSN:01674781
CODEN:BBGSD
CAS:DNA, Protozoan; Pyruvate Dehydrogenase Complex; RNA, Messenger; RNA, Protozoan
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674781_v1309_n1-2_p53_Buscaglia

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Citas:

---------- APA ----------
Buscaglia, C.A., Pollevick, G.D., Veloso, C., Lorca, M., Frasch, A.C.C. & Sánchez, D.O. (1996) . A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi. Biochimica et Biophysica Acta - Gene Structure and Expression, 1309(1-2), 53-57.
http://dx.doi.org/10.1016/S0167-4781(96)00140-6
---------- CHICAGO ----------
Buscaglia, C.A., Pollevick, G.D., Veloso, C., Lorca, M., Frasch, A.C.C., Sánchez, D.O. "A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi" . Biochimica et Biophysica Acta - Gene Structure and Expression 1309, no. 1-2 (1996) : 53-57.
http://dx.doi.org/10.1016/S0167-4781(96)00140-6
---------- MLA ----------
Buscaglia, C.A., Pollevick, G.D., Veloso, C., Lorca, M., Frasch, A.C.C., Sánchez, D.O. "A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi" . Biochimica et Biophysica Acta - Gene Structure and Expression, vol. 1309, no. 1-2, 1996, pp. 53-57.
http://dx.doi.org/10.1016/S0167-4781(96)00140-6
---------- VANCOUVER ----------
Buscaglia, C.A., Pollevick, G.D., Veloso, C., Lorca, M., Frasch, A.C.C., Sánchez, D.O. A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi. BIOCHIM. BIOPHYS. ACTA GENE STRUCT. EXPR. 1996;1309(1-2):53-57.
http://dx.doi.org/10.1016/S0167-4781(96)00140-6