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Abstract:

Proper cell wall assembly is crucial during pollen tube growth. Leucine-rich repeat extensins (LRXs) are extracellular glycoproteins which belong to the hydroxyproline-rich glycoprotein (HRGP) family. They contain a conserved N-terminal leucine-rich repeat (LRR) domain and a highly variable C-terminal extensin domain. Here, we characterized four LRX proteins (LRX8 through LRX11) from pollen of Arabidopsis thaliana. To investigate the role of LRX8-LRX11 in pollen germination and pollen tube growth, multiple T-DNA lrx mutants were obtained. The lrx mutants display abnormal pollen tubes with an irregular deposition of callose and pectin. They also show serious alterations in pollen germination and segregation ratio. Our results suggest that LRXs are involved in ensuring proper cell wall assembly during pollen tube growth. © 2017 Federation of European Biochemical Societies

Registro:

Documento: Artículo
Título:Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth
Autor:Sede, A.R.; Borassi, C.; Wengier, D.L.; Mecchia, M.A.; Estevez, J.M.; Muschietti, J.P.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, “Dr. Héctor Torres” (INGEBI-CONICET), Buenos Aires, Argentina
Fundación Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Instituto de Fisiología, Biología Molecular y Neurociencias, IFIByNE-CONICET, Universidad de Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
CRAG (Centro de Investigación en Agrigenómica), Barcelona, Spain
Palabras clave:callose; cell wall; extensin; LRX; pectin; polarized growth; pollen tube; beta 1,3 glucan; callose; extensin; leucine rich repeat extensin; LRX10 protein; LRX11 protein; LRX8 protein; LRX9 protein; pectin; unclassified drug; callose; extensin protein, plant; glucan; glycoprotein; pectin; plant protein; Arabidopsis thaliana; cell wall; controlled study; Letter; nonhuman; pollen germination; pollen tube growth; pollination; priority journal; seed development; Arabidopsis; cell wall; gene expression regulation; genetics; germination; growth, development and aging; metabolism; molecular cloning; physiology; pollen tube; Arabidopsis; Cell Wall; Cloning, Molecular; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Germination; Glucans; Glycoproteins; Mutagenesis, Insertional; Pectins; Plant Proteins; Pollen Tube
Año:2018
Volumen:592
Número:2
Página de inicio:233
Página de fin:243
DOI: http://dx.doi.org/10.1002/1873-3468.12947
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:beta 1,3 glucan, 9051-97-2; pectin, 9000-69-5; glucan, 9012-72-0, 9037-91-6; callose; extensin protein, plant; Glucans; Glycoproteins; Pectins; Plant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v592_n2_p233_Sede

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Citas:

---------- APA ----------
Sede, A.R., Borassi, C., Wengier, D.L., Mecchia, M.A., Estevez, J.M. & Muschietti, J.P. (2018) . Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth. FEBS Letters, 592(2), 233-243.
http://dx.doi.org/10.1002/1873-3468.12947
---------- CHICAGO ----------
Sede, A.R., Borassi, C., Wengier, D.L., Mecchia, M.A., Estevez, J.M., Muschietti, J.P. "Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth" . FEBS Letters 592, no. 2 (2018) : 233-243.
http://dx.doi.org/10.1002/1873-3468.12947
---------- MLA ----------
Sede, A.R., Borassi, C., Wengier, D.L., Mecchia, M.A., Estevez, J.M., Muschietti, J.P. "Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth" . FEBS Letters, vol. 592, no. 2, 2018, pp. 233-243.
http://dx.doi.org/10.1002/1873-3468.12947
---------- VANCOUVER ----------
Sede, A.R., Borassi, C., Wengier, D.L., Mecchia, M.A., Estevez, J.M., Muschietti, J.P. Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth. FEBS Lett. 2018;592(2):233-243.
http://dx.doi.org/10.1002/1873-3468.12947