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In strawberry, the putative participation of aquaporins should be considered during fruit ripening. Furthermore, the availability of different firmness cultivars in this non-climacteric fruit is a very useful tool to determine their involvement in softening. In a previous work, the cloning of a strawberry fruit-specific aquaporin, FaPIP1;1, which showed an expression profile associated with fruit ripening was reported. Here, FaPIP2;1, an aquaporin subtype of PIP2 was cloned and its functional characterization in Xenopus oocytes determined. The FaPIP2;1 gene encodes a water channel with high water permeability (Pf) that is regulated by cytosolic pH. Interestingly, the co-expression of both FaPIP subtypes resulted in an enhancement of water permeability, showing Pf values that exceeds their individual contribution. The expression pattern of both aquaporin subtypes in two cultivars with contrasting fruit firmness showed that the firmer cultivar (Camarosa) has a higher accumulation of FaPIP1 and FaPIP2 mRNAs during fruit ripening when compared with the softer cultivar (Toyonoka). In conclusion, not only FaPIP aquaporins showed an expression pattern associated with fruit firmness but it was also shown that the enhancement of water transfer through the plasma membrane is coupled to the presence/absence of the co-expression of both subtypes. © 2010 The Author(s).


Documento: Artículo
Título:Cloning, functional characterization, and co-expression studies of a novel aquaporin (FaPIP2;1) of strawberry fruit
Autor:Alleva, K.; Marquez, M.; Villarreal, N.; Mut, P.; Bustamante, C.; Bellati, J.; Martínez, G.; Civello, M.; Amodeo, G.
Filiación:Laboratorio de Biomembranas, Departamento de Fisiología, Universidad de Buenos Aires, Argentina
IIB-INTECH, UNSAM-CONICET, Chascomús, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 4̊Piso, C1428EHA Buenos Aires, Argentina
Palabras clave:Aquaporin; fruit ripening; PIP; strawberry; water transport; aquaporin; complementary DNA; vegetable protein; water; aquaporin; vegetable protein; amino acid sequence; animal; article; cell membrane; chemistry; fruit; genetics; metabolism; molecular cloning; molecular genetics; permeability; strawberry; Xenopus laevis; Fragaria; fruit; metabolism; Amino Acid Sequence; Animals; Aquaporins; Cell Membrane; Cloning, Molecular; DNA, Complementary; Fragaria; Fruit; Molecular Sequence Data; Permeability; Plant Proteins; Water; Xenopus laevis; Fragaria x ananassa; Amino Acid Sequence; Animals; Aquaporins; Cell Membrane; Cloning, Molecular; DNA, Complementary; Fragaria; Fruit; Molecular Sequence Data; Permeability; Plant Proteins; Water; Xenopus laevis
Página de inicio:3935
Página de fin:3945
Título revista:Journal of Experimental Botany
Título revista abreviado:J. Exp. Bot.
CAS:aquaporin, 215587-75-0; water, 7732-18-5; Aquaporins; DNA, Complementary; Plant Proteins; Water, 7732-18-5; Aquaporins; DNA, Complementary; Plant Proteins; Water


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---------- APA ----------
Alleva, K., Marquez, M., Villarreal, N., Mut, P., Bustamante, C., Bellati, J., Martínez, G.,..., Amodeo, G. (2010) . Cloning, functional characterization, and co-expression studies of a novel aquaporin (FaPIP2;1) of strawberry fruit. Journal of Experimental Botany, 61(14), 3935-3945.
---------- CHICAGO ----------
Alleva, K., Marquez, M., Villarreal, N., Mut, P., Bustamante, C., Bellati, J., et al. "Cloning, functional characterization, and co-expression studies of a novel aquaporin (FaPIP2;1) of strawberry fruit" . Journal of Experimental Botany 61, no. 14 (2010) : 3935-3945.
---------- MLA ----------
Alleva, K., Marquez, M., Villarreal, N., Mut, P., Bustamante, C., Bellati, J., et al. "Cloning, functional characterization, and co-expression studies of a novel aquaporin (FaPIP2;1) of strawberry fruit" . Journal of Experimental Botany, vol. 61, no. 14, 2010, pp. 3935-3945.
---------- VANCOUVER ----------
Alleva, K., Marquez, M., Villarreal, N., Mut, P., Bustamante, C., Bellati, J., et al. Cloning, functional characterization, and co-expression studies of a novel aquaporin (FaPIP2;1) of strawberry fruit. J. Exp. Bot. 2010;61(14):3935-3945.