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Abstract:

We report the cloning and characterization of rat α10, a previously unidentified member of the nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by the α10 nAChR subunit gene is most similar to the rat α9 nAChR, and both α9 and α10 subunit genes are transcribed in adult rat mechanosensory hair cells. Injection of Xenopus laevis oocytes with α10 cRNA alone or in pairwise combinations with either α2-α6 or β2-β4 subunit cRNAs yielded no detectable ACh-gated currents. However, coinjection of α9 and α10 cRNAs resulted in the appearance of an unusual nAChR subtype. Compared with homomeric α9 channels, the α9α10 nAChR subtype displays faster and more extensive agonist-mediated desensitization, a distinct current-voltage relationship, and a biphasic response to changes in extracellular Ca2+ ions. The pharmacological profiles of homomeric α9 and heteromeric α9α10 nAChRs are essentially indistinguishable and closely resemble those reported for endogenous cholinergic eceptors found in vertebrate hair cells. Our data suggest that efferent modulation of hair cell function occurs, at least in part, through heteromeric nAChRs assembled from both α9 and α10 subunits.

Registro:

Documento: Artículo
Título:α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
Autor:Elgoyhen, A.B.; Vetter, D.E.; Katz, E.; Rothlin, C.V.; Heinemann, S.F.; Boulter, J.
Filiación:Instituto de Investigaciones en Ingeniería, Genética y Biología Molecular, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Salk Institute for Biological Studies, Molecular Neurobiology Laboratory, San Diego, CA 92037, United States
Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
University of California, Department of Psychiatry and Biobehavioral Sciences, University of California Los Angeles Brain Research Institute, Los Angeles, CA 90095, United States
University of California, Macdonald Research Laboratory Building, 675 Charles Young Drive, Los Angeles, CA 90095, United States
Palabras clave:1,1 dimethyl 4 phenylpiperazinium; acetylcholine; alpha bungarotoxin; atropine; bicuculline; carbachol; cholinergic receptor; cholinergic receptor blocking agent; cholinergic receptor stimulating agent; muscarine; nicotine; nicotinic agent; nicotinic receptor; nicotinic receptor blocking agent; oxotremorine; receptor subunit; strychnine; tropisetron; tubocurarine chloride; nicotinic receptor; animal cell; article; cholinergic receptor blocking; cochlea; dose response; drug receptor binding; extracellular calcium; hair cell; human; molecular cloning; multigene family; nonhuman; nucleotide sequence; oocyte; priority journal; rat; receptor down regulation; vestibule; Xenopus laevis; amino acid sequence; animal; cochlea; cytology; female; gene expression; genetics; metabolism; molecular genetics; mouse; nucleotide sequence; physiology; vestibule; Animalia; Mammalia; Strychnos toxifera; Vertebrata; Xenopus laevis; Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Cochlea; Female; Gene Expression; Hair Cells; Hair Cells, Vestibular; Humans; Mice; Molecular Sequence Data; Rats; Receptors, Nicotinic; Vestibule; Xenopus laevis
Año:2001
Volumen:98
Número:6
Página de inicio:3501
Página de fin:3506
DOI: http://dx.doi.org/10.1073/pnas.051622798
Título revista:Proceedings of the National Academy of Sciences of the United States of America
Título revista abreviado:Proc. Natl. Acad. Sci. U. S. A.
ISSN:00278424
CODEN:PNASA
CAS:Receptors, Nicotinic
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00278424_v98_n6_p3501_Elgoyhen.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v98_n6_p3501_Elgoyhen

Referencias:

  • Hudspeth, A.J., (1989) Nature (London), 341, pp. 397-404
  • Fuchs, P.A., (1996) Curr. Opin. Neurobiol., 6, pp. 514-519
  • Guth, P.S., Norris, C.H., (1996) Hear. Res., 98, pp. 1-8
  • Warr, W.B., (1992) Organization of Olivocochlaer Efferent Systems in Mammals/The Mammalian Auditory Pathway: Neuroanatomy, , Springer, New York
  • Guinan J.J., Jr., Stankovic, K.M., (1996) J. Acoust. Soc. Am., 100, pp. 1680-1690
  • Ulfendahl, M., Flock, A., (1998) Curr. Opin. Neurobiol., 8, pp. 475-479
  • Housley, G.D., Ashmore, J.F., (1991) Proc. R. Soc. London, 244, pp. 161-167
  • Fuchs, P.A., Murrow, B.W., (1992) J. Neurosci., 12, pp. 800-809
  • Sridhar, T.S., Liberman, M.C., Brown, M.C., Sewell, W.F., (1995) J. Neurosci., 15, pp. 3667-3678
  • Murugasu, E., Russell, I.J., (1996) J. Neurosci., 16, pp. 325-332
  • Eybalin, M., (1993) Physiol. Rev., 73, pp. 309-373
  • Yuhas, W.A., Fuchs, P.A., (1999) J. Comp. Physiol., 185, pp. 455-462
  • Elgoyhen, A.B., Johnson, D.S., Boulter, J., Vetter, D.E., Heinemann, S., (1994) Cell, 79, pp. 705-715
  • Rothlin, C.V., Katz, E., Verbitsky, M., Elgoyhen, A.B., (1999) Mol. Pharmacol., 55, pp. 248-254
  • Katz, E., Verbitsky, M., Rothlin, C.V., Vetter, D.E., Heinemann, S.F., Elgoyhen, A.B., (2000) Hear. Res., 141, pp. 117-128
  • Verbitsky, M., Rothlin, C.V., Katz, E., Elgoyhen, A.B., (2000) Neuropharmacology, 39, pp. 2515-2524
  • Dulon, D., Lenoir, M., (1996) Eur. J. Neurosci., 8, pp. 1945-1952
  • Blanchet, C., Eróstegui, C., Sugasawa, M., Dulon, D., (1996) J. Neurosci., 16, pp. 2574-2584
  • Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J., (1990) J. Mol. Biol., 215, pp. 403-410
  • Zuo, J., Treadaway, J., Buckner, T.W., Fritzsch, B., (1999) Proc. Natl. Acad. Sci. USA, 96, pp. 14100-14105
  • Fuchs, P.A., Murrow, B.W., (1992) Proc. R. Soc. London, 248, pp. 35-40
  • Erostegui, C., Norris, C.H., Bobbin, R.P., (1994) Hear. Res., 74, pp. 135-147
  • Vetter, D.E., Liberman, M.C., Mann, J., Barhanin, J., Boulter, J., Brown, M.C., Saffiote-Kolman, J., Elgoyhen, A.B., (1999) Neuron, 23, pp. 93-103
  • McNiven, A.I., Yuhas, W.A., Fuchs, P.A., (1996) Audit. Neurosci., 2, pp. 63-77
  • Morley, B.J., Li, H.S., Hiel, H., Drescher, D.G., Elgoyhen, A.B., (1998) Mol. Brain. Res., 53, pp. 78-87
  • Glowatzki, E., Fuchs, P.A., (2000) Science, 288, pp. 2366-2368

Citas:

---------- APA ----------
Elgoyhen, A.B., Vetter, D.E., Katz, E., Rothlin, C.V., Heinemann, S.F. & Boulter, J. (2001) . α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells. Proceedings of the National Academy of Sciences of the United States of America, 98(6), 3501-3506.
http://dx.doi.org/10.1073/pnas.051622798
---------- CHICAGO ----------
Elgoyhen, A.B., Vetter, D.E., Katz, E., Rothlin, C.V., Heinemann, S.F., Boulter, J. "α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells" . Proceedings of the National Academy of Sciences of the United States of America 98, no. 6 (2001) : 3501-3506.
http://dx.doi.org/10.1073/pnas.051622798
---------- MLA ----------
Elgoyhen, A.B., Vetter, D.E., Katz, E., Rothlin, C.V., Heinemann, S.F., Boulter, J. "α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells" . Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 6, 2001, pp. 3501-3506.
http://dx.doi.org/10.1073/pnas.051622798
---------- VANCOUVER ----------
Elgoyhen, A.B., Vetter, D.E., Katz, E., Rothlin, C.V., Heinemann, S.F., Boulter, J. α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells. Proc. Natl. Acad. Sci. U. S. A. 2001;98(6):3501-3506.
http://dx.doi.org/10.1073/pnas.051622798