Abstract:
The retrograde transport of nerve growth factor (NGF) in neurite-like processes of living differentiated PC12 cells was studied using streptavidin-quantum dots (QDs) coupled to monobiotin-NGF. These reagents were active in differentiation, binding, internalization, and transport. Ten-35% of the QD-NGF-receptor complexes were mobile. Quantitative single particle tracking revealed a bidirectional step-like motion, requiring intact microtubules, with a net retrograde velocity of 0.054 ± 0.020 μm/s. Individual runs had a mean velocity of ∼0.15 μm/s at room temperature, and the run times were exponentially distributed. The photostability and brightness of QDs permit extended real-time analysis of individual QDbNGF- receptor complexes trafficking within neurites. © 2007 Federation of European Biochemical Societies.
Registro:
Documento: |
Artículo
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Título: | Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths |
Autor: | Echarte, M.M.; Bruno, L.; Arndt-Jovin, D.J.; Jovin, T.M.; Pietrasanta, L.I. |
Filiación: | Centro de Microscopías Avanzadas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon I, Ciudad Universitaria, Intendente Guiraldes 2160, C1428EHA Buenos Aires, Argentina Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon I, Ciudad Universitaria, Intendente Guiraldes 2160, C1428EHA Buenos Aires, Argentina Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37070 Göttingen, Germany Consejo Nacional de Investigaciones Científicas y Técnicas, C1033AAJ Buenos Aires, Argentina
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Palabras clave: | Live cell imaging; Neurotrophins; NGF; Quantum dots; Retrograde axonal transport; nerve growth factor receptor; quantum dot; streptavidin; animal cell; article; cell differentiation; cell labeling; controlled study; imaging; internalization; microtubule; mouse; neurite; nonhuman; priority journal; protein binding; protein transport; quantitative assay; rat; room temperature; velocity; Animals; Chromogenic Compounds; Endocytosis; Microtubules; Multiprotein Complexes; Neurites; PC12 Cells; Protein Binding; Protein Transport; Quantum Dots; Rats; Receptor, Nerve Growth Factor; Sensitivity and Specificity; Staining and Labeling; Substrate Specificity |
Año: | 2007
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Volumen: | 581
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Número: | 16
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Página de inicio: | 2905
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Página de fin: | 2913
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DOI: |
http://dx.doi.org/10.1016/j.febslet.2007.05.041 |
Título revista: | FEBS Letters
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Título revista abreviado: | FEBS Lett.
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ISSN: | 00145793
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CODEN: | FEBLA
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CAS: | streptavidin, 9013-20-1; Chromogenic Compounds; Multiprotein Complexes; Receptor, Nerve Growth Factor
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PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00145793_v581_n16_p2905_Echarte.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v581_n16_p2905_Echarte |
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Citas:
---------- APA ----------
Echarte, M.M., Bruno, L., Arndt-Jovin, D.J., Jovin, T.M. & Pietrasanta, L.I.
(2007)
. Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths. FEBS Letters, 581(16), 2905-2913.
http://dx.doi.org/10.1016/j.febslet.2007.05.041---------- CHICAGO ----------
Echarte, M.M., Bruno, L., Arndt-Jovin, D.J., Jovin, T.M., Pietrasanta, L.I.
"Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths"
. FEBS Letters 581, no. 16
(2007) : 2905-2913.
http://dx.doi.org/10.1016/j.febslet.2007.05.041---------- MLA ----------
Echarte, M.M., Bruno, L., Arndt-Jovin, D.J., Jovin, T.M., Pietrasanta, L.I.
"Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths"
. FEBS Letters, vol. 581, no. 16, 2007, pp. 2905-2913.
http://dx.doi.org/10.1016/j.febslet.2007.05.041---------- VANCOUVER ----------
Echarte, M.M., Bruno, L., Arndt-Jovin, D.J., Jovin, T.M., Pietrasanta, L.I. Quantitative single particle tracking of NGF-receptor complexes: Transport is bidirectional but biased by longer retrograde run lengths. FEBS Lett. 2007;581(16):2905-2913.
http://dx.doi.org/10.1016/j.febslet.2007.05.041