Abstract:
Kinetic studies were carried out using purified porphobilinogenase and deaminase preparations in the presence and absence of ammonium ions. It has been found in plots of v versus [S] that a deviation from the Michaelis-Menten hyperbola occurs with both enzymes; double-reciprocal plots were concave downward; Rs values were greater than 81; and in some cases the Hill coefficient was less than 1, indicating negative homotropic kinetics. Evidence also suggested that porphobilinogenase contains at least two substrate-binding sites per molecule of enzyme. It has also been found that ammonium ions act competitively on the first reaction of the porphobilinogenase. © 1970.
Referencias:
- Sancovich, del C. Batlle, Grinstein, (1969) Biochim. Biophys. Acta, 191, p. 130
- Lockwood, Rimington, (1957) Biochem. J., 67, p. 8P
- Sancovich, del C. Batlle, Grinstein, (1969) FEBS Letters, 3, p. 223
- Bogorad, ENZYMATIC MECHANISMS IN PORPHYRIN SYNTHESIS: POSSIBLE ENZYMATIC BLOCKS IN PORPHYRIAS* (1963) Annals of the New York Academy of Sciences, 104, p. 676
- Llambias, del C. Batlle, (1970) FEBS Letters, 6, p. 285
- E. B. C. Llambias and A. M. del C. Batlle, Biochem. J., in the press; Sancovich, Ferramolo, del C. Batlle, Grinstein, (1970) Methods in Enzymology, 17, p. 220. , 2nd ed., H. Tabor, C. Tabor, Academic Press, New York
- Hill, The Combinations of Haemoglobin with Oxygen and with Carbon Monoxide. I. (1913) Biochem J, 7, p. 471
- Koshland, Jr., Nemethy, Filmer, (1966) Biochemistry, 5, p. 365
- Bogorad, (1960) Comparative Biochemistry of Photoreactive Systems, p. 22. , 2nd ed., M.B. Allen, Academic Press, New York and London
- Niesselbaum, Bodansky, (1961) J. Biol. Chem., 236, p. 323
- Gerhart, Pardee, (1962) J. Biol. Chem., 237, p. 891
- Okazaki, Kornberg, (1964) J. Biol. Chem., 239, p. 275
- Iwatsuki, Okazaki, (1967) J. Mol. Biol., 29, p. 155
- Datta, Gest, (1965) J. Biol. Chem., 240, p. 3023
- Corman, Kaplan, (1965) Biochemistry, 4, p. 2175
- Corman, Kaplan, (1967) J. Biol. Chem., 242, p. 2840
- Wiggert, Cohen, (1966) J. Biol. Chem., 241, p. 210
- Bernofsky, Utter, (1967) Biochim. Biophys. Acta, 132, p. 244
- LéJohn, Jackson, Allosteric interactions of a regulatory nicotinamide adenine dinucleotide-specific glutamate dehydrogenase from Blastocladiella. A molecular model for the enzyme. (1968) J Biol Chem, 243, p. 3447
- Corwin, Fanning, (1968) J. Biol. Chem., 243, p. 3517
- Levitzky, Koshland, Jr., NEGATIVE COOPERATIVITY IN REGULATORY ENZYMES (1969) Proceedings of the National Academy of Sciences, 62, p. 1121. , 2nd ed
- Kirtley, Koshland, Jr., (1967) J. Biol. Chem., 242, p. 4192
- Conway, Koshland, Jr., (1968) Biochemistry, 7, p. 4011
- Dalziel, Engel, (1968) FEBS Letters, 1, p. 349
- Engel, Dalziel, (1969) Biochem. J., 115, p. 621
- Frieden, (1964) J. Biol. Chem., 239, p. 3522
- Frieden, (1959) J. Biol. Chem., 234, p. 815
- Frieden, (1967) J. Biol. Chem., 242, p. 4045
Citas:
---------- APA ----------
Llambías, E.B.C. & Del C. Batlle, A.M.
(1970)
. Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies. BBA - Enzymology, 220(3), 552-559.
http://dx.doi.org/10.1016/0005-2744(70)90285-8---------- CHICAGO ----------
Llambías, E.B.C., Del C. Batlle, A.M.
"Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies"
. BBA - Enzymology 220, no. 3
(1970) : 552-559.
http://dx.doi.org/10.1016/0005-2744(70)90285-8---------- MLA ----------
Llambías, E.B.C., Del C. Batlle, A.M.
"Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies"
. BBA - Enzymology, vol. 220, no. 3, 1970, pp. 552-559.
http://dx.doi.org/10.1016/0005-2744(70)90285-8---------- VANCOUVER ----------
Llambías, E.B.C., Del C. Batlle, A.M. Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies. 1970;220(3):552-559.
http://dx.doi.org/10.1016/0005-2744(70)90285-8