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Abstract:

Kinetic studies were carried out using purified porphobilinogenase and deaminase preparations in the presence and absence of ammonium ions. It has been found in plots of v versus [S] that a deviation from the Michaelis-Menten hyperbola occurs with both enzymes; double-reciprocal plots were concave downward; Rs values were greater than 81; and in some cases the Hill coefficient was less than 1, indicating negative homotropic kinetics. Evidence also suggested that porphobilinogenase contains at least two substrate-binding sites per molecule of enzyme. It has also been found that ammonium ions act competitively on the first reaction of the porphobilinogenase. © 1970.

Registro:

Documento: Artículo
Título:Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies
Autor:Llambías, E.B.C.; Del C. Batlle, A.M.
Filiación:Cátedra de Química Biológica I, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Perú, 272 Buenos Aires, Argentina
Palabras clave:ammonium derivative; article; binding site; Ammonium Compounds; Binding Sites
Año:1970
Volumen:220
Número:3
Página de inicio:552
Página de fin:559
DOI: http://dx.doi.org/10.1016/0005-2744(70)90285-8
Título revista:BBA - Enzymology
ISSN:00052744
CAS:Ammonium Compounds
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00052744_v220_n3_p552_Llambias.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052744_v220_n3_p552_Llambias

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Citas:

---------- APA ----------
Llambías, E.B.C. & Del C. Batlle, A.M. (1970) . Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies. BBA - Enzymology, 220(3), 552-559.
http://dx.doi.org/10.1016/0005-2744(70)90285-8
---------- CHICAGO ----------
Llambías, E.B.C., Del C. Batlle, A.M. "Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies" . BBA - Enzymology 220, no. 3 (1970) : 552-559.
http://dx.doi.org/10.1016/0005-2744(70)90285-8
---------- MLA ----------
Llambías, E.B.C., Del C. Batlle, A.M. "Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies" . BBA - Enzymology, vol. 220, no. 3, 1970, pp. 552-559.
http://dx.doi.org/10.1016/0005-2744(70)90285-8
---------- VANCOUVER ----------
Llambías, E.B.C., Del C. Batlle, A.M. Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies. 1970;220(3):552-559.
http://dx.doi.org/10.1016/0005-2744(70)90285-8