Abstract:
Specific binding was linear up to 3 mg of protein in the incubation tube. Melatonin binding was pH-dependent the maximum being observed at pH 7.4. By adding 6 mM Ca++ to the buffer a 2-fold increase of specific binding was observed. Scatchard analysis revealed a population of high affinity binding sites with a Kd=(1.2±0.4)x10-8 M in 3 successive experiments.
Registro:
| Documento: |
Artículo
|
| Título: | High affinity binding of melatonin in bovine medial basal hypothalamus |
| Autor: | Cardinali, D.P.; Vacas, M.I.; Estevez Boyer, E. |
| Filiación: | Cent. Est. Farmacol., 1428 Buenos Aires, Argentina
|
| Palabras clave: | melatonin; animal experiment; cattle; central nervous system; endocrine system; hypothalamus |
| Año: | 1978
|
| Volumen: | 6
|
| Número: | 9
|
| Página de inicio: | 357
|
| Título revista: | IRCS Medical Science: Biochemistry
|
| Título revista abreviado: | IRCS MED. SCI. BIOCHEM.
|
| CODEN: | IMSBD
|
| CAS: | melatonin, 73-31-4
|
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18622_v6_n9_p357_Cardinali |
Citas:
---------- APA ----------
Cardinali, D.P., Vacas, M.I. & Estevez Boyer, E.
(1978)
. High affinity binding of melatonin in bovine medial basal hypothalamus. IRCS Medical Science: Biochemistry, 6(9), 357.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18622_v6_n9_p357_Cardinali [ ]
---------- CHICAGO ----------
Cardinali, D.P., Vacas, M.I., Estevez Boyer, E.
"High affinity binding of melatonin in bovine medial basal hypothalamus"
. IRCS Medical Science: Biochemistry 6, no. 9
(1978) : 357.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18622_v6_n9_p357_Cardinali [ ]
---------- MLA ----------
Cardinali, D.P., Vacas, M.I., Estevez Boyer, E.
"High affinity binding of melatonin in bovine medial basal hypothalamus"
. IRCS Medical Science: Biochemistry, vol. 6, no. 9, 1978, pp. 357.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18622_v6_n9_p357_Cardinali [ ]
---------- VANCOUVER ----------
Cardinali, D.P., Vacas, M.I., Estevez Boyer, E. High affinity binding of melatonin in bovine medial basal hypothalamus. IRCS MED. SCI. BIOCHEM. 1978;6(9):357.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18622_v6_n9_p357_Cardinali [ ]
