By means of controlled mechanical shearing it was possible to fractionate human breast tumor chromatin in 'active' and 'inactive' species, according to their in vitro template activity, using E. coli RNA polymerase. The 'active' molecules can be resolved in 3 well defined regions in an exponential sucrose gradient, showing approximately 300 times the efficiency for synthesizing ribonucleic acid, relative to the chromatin which migrated fastest. This technique could provide the initial tool to isolate eu-and heterochromatin from native human chromatin.
| Documento: | Artículo |
| Título: | Transcriptional features of fractionated human breast tumor chromatin |
| Autor: | Thierbach, L.I.A.; Rosemblit, N.; Pahn, C.R.; Baldi, A. |
| Filiación: | Inst. Biol. Med. Exp., 1428 Buenos Aires, Argentina |
| Palabras clave: | rna; rna polymerase; breast; breast cancer; chromatin; escherichia coli; euchromatin; genetic transcription; heterochromatin; human cell; in vitro study; theoretical study; Breast Neoplasms; Chromatin; Deoxyribonucleoproteins; DNA, Neoplasm; Female; Human; RNA Polymerase I; RNA, Neoplasm; Support, Non-U.S. Gov't; Transcription, Genetic |
| Año: | 1979 |
| Volumen: | 29 |
| Número: | 1 |
| Página de inicio: | 81 |
| Página de fin: | 85 |
| Título revista: | Acta Physiologica Latino-Americana |
| Título revista abreviado: | ACTA PHYSIOL. LAT.-AM. |
| CODEN: | APLTA |
| CAS: | RNA polymerase, 9014-24-8; RNA, 63231-63-0; Chromatin; Deoxyribonucleoproteins; DNA, Neoplasm; RNA Polymerase I, EC 2.7.7.-; RNA, Neoplasm |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18558_v29_n1_p81_Thierbach |
