Abstract:
In this chapter are reported the experimental strategies and details to assess alternative splicing (AS) in mammalian cells that are transfected with reporter minigenes carrying different promoters, or where artificial mutants of RNA polymerase II are used to generate RNA from these reporters. These techniques were applied successfully to demonstrate the coupling between transcription and AS, and to elucidate the roles of the carboxy-terminal domain of RNA polymerase II in the recruitment of splicing factors, and of the rate of transcriptional elongation on splice site selection. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA.
Registro:
| Documento: |
Parte de libro
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| Título: | Coupled Promoter Splicing Systems |
| Autor: | Muñoz, M.J.; de la Mata, M.; Kornblihtt, A.R. |
| Filiación: | Universidad de Buenos Aires, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales IFIBYNE-CONICET, Pabellón 2, piso 2, C1428EHA Buenos Aires, Argentina
Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
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| Palabras clave: | Coupling; Reporter minigene transfections; RT-PCR; Transcription and alternative splicing |
| Año: | 2012
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| Página de inicio: | 400
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| Página de fin: | 407
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| DOI: |
http://dx.doi.org/10.1002/9783527636778.ch37 |
| Título revista: | Alternative pre-mRNA Splicing: Theory and Protocols
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| Título revista abreviado: | Altern. pre-mRNA Splicing: Theory and Protocols
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| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97835273_v_n_p400_Munoz |
Referencias:
- Kornblihtt, A.R., Promoter usage and alternative splicing (2005) Curr. Opin. Cell Biol., 17, pp. 262-268
- Cramer, P., Pesce, C.G., Baralle, F.E., Kornblihtt, A.R., Functional association between promoter architecture and transcript alternative splicing (1997) Proc. Natl Acad. Sci. USA, 94, pp. 11456-11460
- Kadener, S., Cramer, P., Nogués, G., Cazalla, D., de la Mata, M., Fededa, J.P., Werbajh, S., Kornblihtt, A.R., Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing (2001) EMBO J., 20, pp. 5759-5768
- de la Mata, M., Alonso, C.R., Kadener, S., Fededa, J.P., Blaustein, M., Pelisch, F., Cramer, P., Kornblihtt, A.R., A slow RNA polymerase II affects alternative splicing in vivo (2003) Mol. Cell, 12, pp. 525-532
- Gossen, M., Bujard, H., Tight control of gene expression in mammalian cells by tetracycline-responsive promoters (1992) Proc. Natl Acad. Sci. USA, 89, pp. 5547-5551
- de la Mata, M., Kornblihtt, A.R., RNA polymerase II C-terminal domain mediates regulation of alternative splicing by SRp20 (2006) Nat. Struct. Mol. Biol., 13, pp. 973-980
- Blau, J., Xiao, H., McCracken, S., O'Hare, P., Greenblatt, J., Bentley, D., Three functional classes of transcriptional activation domain (1996) Mol. Cell. Biol., 16, pp. 2044-2055
- Gerber, H.P., Hagmann, M., Seipel, K., Georgiev, O., West, M.A., Litingtung, Y., Schaffner, W., Corden, J.L., RNA polymerase II C-terminal domain required for enhancer-driven transcription (1995) Nature, 374, pp. 660-662
Citas:
---------- APA ----------
Muñoz, M.J., de la Mata, M. & Kornblihtt, A.R.
(2012)
. Coupled Promoter Splicing Systems. Alternative pre-mRNA Splicing: Theory and Protocols, 400-407.
http://dx.doi.org/10.1002/9783527636778.ch37---------- CHICAGO ----------
Muñoz, M.J., de la Mata, M., Kornblihtt, A.R.
"Coupled Promoter Splicing Systems"
. Alternative pre-mRNA Splicing: Theory and Protocols
(2012) : 400-407.
http://dx.doi.org/10.1002/9783527636778.ch37---------- MLA ----------
Muñoz, M.J., de la Mata, M., Kornblihtt, A.R.
"Coupled Promoter Splicing Systems"
. Alternative pre-mRNA Splicing: Theory and Protocols, 2012, pp. 400-407.
http://dx.doi.org/10.1002/9783527636778.ch37---------- VANCOUVER ----------
Muñoz, M.J., de la Mata, M., Kornblihtt, A.R. Coupled Promoter Splicing Systems. Altern. pre-mRNA Splicing: Theory and Protocols. 2012:400-407.
http://dx.doi.org/10.1002/9783527636778.ch37