Parte de libro

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor


Documento: Parte de libro
Título:Three-Dimensional Particle Tracking in a Laser Scanning Fluorescence Microscope
Autor:Levi, V.; Gratton, E.
Filiación:University of Buenos Aires, Departments of Physics and Biological Chemistry, Pabellón I - Ciudad Universitaria, Intendente Guiraldes 2160, Buenos Aires C1428EHA, Argentina
University of California at Irvine, Laboratory for Fluorescence Dynamics, 3210 Natural Sciences II, Irvine, CA 92697-2715, United States
Palabras clave:Background noise; Chromatin dynamics; Fluorescence microscopy; Laser scanning microscopy; Single-particle tracking; Three-dimensional tracking; Two-photon excitation microscopy
Página de inicio:1
Página de fin:24
Título revista:Single Particle Tracking and Single Molecule Energy Transfer
Título revista abreviado:Single Particle Tracking and Single Mol. Energy Transf.


  • Weiss, S., Fluorescence spectroscopy of single biomolecules (1999) Science, 283, pp. 1676-1683
  • Axelrod, D., Koppel, D.E., Schlessinger, J., Elson, E., Webb, W.W., Mobility measurement by analysis of fluorescence photobleaching recovery kinetics (1976) Biophys. J., 16, pp. 1055-1069
  • Magde, D., Elson, E., Webb, W.W., Thermodynamics fluctuations in a reactin system: measurement by fluorescence correlation spectroscopy (1972) Phys. Rev. Lett., 29, pp. 705-708
  • Reits, E.A., Neefjes, J.J., From fixed to FRAP: measuring protein mobility and activity in living cells (2001) Nat. Cell Biol, 3, pp. E145-E147
  • Ruan, Q., Cheng, M.A., Levi, M., Gratton, E., Mantulin, W.W., Spatial-temporal studies of membrane dynamics: scanning fluorescence correlation spectroscopy (SFCS) (2004) Biophys. J., 87, pp. 1260-1267
  • Digman, M.A., Brown, C.M., Sengupta, P., Wiseman, P.W., Horwitz, A.R., Gratton, E., Measuring fast dynamics in solutions and cells with a laser scanning microscope (2005) Biophys. J., 89, pp. 1317-1327
  • Hebert, B., Costantino, S., Wiseman, P.W., Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells (2005) Biophys. J., 88, pp. 3601-3614
  • Saxton, M.J., Jacobson, K., Single-particle tracking: applications to membrane dynamics (1997) Annu. Rev. Biophys. Biomol. Struct., 26, pp. 373-399
  • Saxton, M.J., Anomalous diffusion due to obstacles: a Monte Carlo study (1994) Biophys. J., 66, pp. 394-401
  • Saxton, M.J., Single-particle tracking: models of directed transport (1994) Biophys. J., 67, pp. 2110-2119
  • Saxton, M.J., Single-particle tracking: the distribution of diffusion coefficients (1997) Biophys. J., 72, pp. 1744-1753
  • Saxton, M.J., Anomalous diffusion due to binding: a Monte Carlo study (1996) Biophys. J., 70, pp. 1250-1262
  • Saxton, M.J., Lateral diffusion in an archipelago. Single-particle diffusion (1993) Biophys. J., 64, pp. 1766-1780
  • Saxton, M.J., Single-particle tracking: effects of corrals (1995) Biophys. J., 69, pp. 389-398
  • Qian, H., Sheetz, M.P., Elson, E.L., Single particle tracking. Analysis of diffusion and flow in two-dimensional systems (1991) Biophys. J., 60, pp. 910-921
  • Gelles, J., Schnapp, B.J., Sheetz, M.P., Tracking kinesin-driven movements with nanometre-scale precision (1988) Nature, 331, pp. 450-453
  • Lee, G.M., Ishihara, A., Jacobson, K., Direct observation of brownian motion of lipids in a membrane (1991) Proc. Natl Acad. Sci. USA, 88, pp. 6274-6278
  • Geerts, H., De Brabander, M., Nuydens, R., Nanovid microscopy (1991) Nature, 351, pp. 765-766
  • Geerts, H., De Brabander, M., Nuydens, R., Geuens, S., Moeremans, M., De Mey, J., Hollenbeck, P.J., Nanovid tracking: a new automatic method for the study of mobility in living cells based on colloidal gold and video microscopy (1987) Biophys. J., 52, pp. 775-782
  • Levi, V., Gratton, E., Exploring dynamics in living cells by tracking single particles (2007) Cell Biochem. Biophys., 48, pp. 1-15
  • Carter, B., Shubeita, G., Gross, S., Tracking single particles: a user-friendly quantitative evaluation (2005) Phys. Biol., 2, pp. 60-72
  • Cheezum, M.K., Walker, W.F., Guilford, W.H., Quantitative comparison of algorithms for tracking single fluorescent particles (2001) Biophys. J., 81, pp. 2378-2388
  • Yildiz, A., Forkey, J.N., McKinney, S.A., Ha, T., Goldman, Y.E., Selvin, P.R., Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization (2003) Science, 300, pp. 2061-2065
  • Yildiz, A., Tomishige, M., Vale, R.D., Selvin, P.R., Kinesin walks hand-over-hand (2004) Science, 303, pp. 676-678
  • Levi, V., Serpinskaya, A.S., Gratton, E., Gelfand, V., Organelle transport along microtubules in Xenopus melanophores: evidence for cooperation between multiple motors (2006) Biophys. J., 90, pp. 318-327
  • Fujiwara, T., Ritchie, K., Murakoshi, H., Jacobson, K., Kusumi, A., Phospholipids undergo hop diffusion in compartmentalized cell membrane (2002) J. Cell Biol., 157, pp. 1071-1081
  • Kusumi, A., Nakada, C., Ritchie, K., Murase, K., Suzuki, K., Murakoshi, H., Kasai, R.S., Fujiwara, T., Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: high-speed single-molecule tracking of membrane molecules (2005) Annu. Rev. Biophys. Biomol. Struct., 34, pp. 351-378
  • Murase, K., Fujiwara, T., Umemura, Y., Suzuki, K., Iino, R., Yamashita, H., Saito, M., Kusumi, A., Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques (2004) Biophys. J., 86, pp. 4075-4093
  • Thompson, R.E., Larson, D.R., Webb, W.W., Precise nanometer localization analysis for individual fluorescent probes (2002) Biophys. J., 82, pp. 2775-2783
  • Sheppard, C.J.R., Shotton, D.M., Confocal Laser Scanning Microscopy (1997), Springer-Verlag New York Inc., New York; Levi, V., Gelfand, V.I., Serpinskaya, A.S., Gratton, E., Melanosomes transported by myosin-V in Xenopus melanophores perform slow 35 nm steps (2006) Biophys. J., 90, pp. L7-L9
  • Spring, K.R., Cameras for digital microscopy (2003), 72, pp. 87-132. , in Digital Microscopy: A Second Edition of Video Microscopy, (eds G. Sluder and D.E. Wolf), Elsevier Academic Press, Amsterdam; Oshiro, M., Moomaw, B., Cooled vs. intensified vs. electron bombardment CCD cameras-applications and relative advantages (2003), 72, pp. 133-156. , in Digital Microscopy: A Second Edition of Video Microscopy, (eds G. Sluder and D.E. Wolf), Elsevier Academic Press, Amsterdam; Berland, K.M., Jacobson, K.A., Frenche, T., Rajfur, Z., Electronic cameras for low-light microscopy (2003), 72, pp. 103-132. , in Digital Microscopy: A Second Edition of Video Microscopy, (eds G. Sluder and D.E. Wolf), Elsevier Academic Press, Amsterdam; Kural, C., Serpinskaya, A.S., Chou, Y., Goldman, R.D., Gelfand, V.I., Selvin, P.R., Tracking melanosomes inside a cell to study molecular motors and their interaction (2007) P roc. Natl Acad. Sci. USA, 104, pp. 5378-5382
  • Graf, R., Rietdorf, J., Zimmermann, T., Live cell spinning disk microscopy (2005) Adv. Biochem. Eng. Biotechnol., 95, pp. 57-75
  • Arhel, N., Genovesio, A., Kim, K.A., Miko, S., Perret, E., Olivo-Marin, J.C., Shorte, S., Charneau, P., Quantitative four-dimensional tracking of cytoplasmic and nuclear HIV-1 complexes (2006) N at. Methods, 3, pp. 817-824
  • Lange, S., Katayama, Y., Schmid, M., Burkacky, O., Brauchle, C., Lamb, D.C., Jansen, R., Simultaneous transport of different localized mRNA species revealed by live-cell imaging (2008) Traffic, 9, pp. 1256-1267
  • Wazawa, T., Ueda, M., Total internal reflection fluorescence microscopy in single molecule nanobioscience (2005) Adv. Biochem. Eng. Biotechnol., 95, pp. 77-106
  • Axelrod, D., Total internal reflection fluorescence microscopy in cell biology (2001) Traffic, 2, pp. 764-774
  • Sako, Y., Minoghchi, S., Yanagida, T., Single-molecule imaging of EGFR signalling on the surface of living cells (2000) Nat. Cell Biol., 2, pp. 168-172
  • Vale, R.D., Funatsu, T., Pierce, D.W., Romberg, L., Harada, Y., Yanagida, T., Direct observation of single kinesin molecules moving along microtubules (1996) Nature, 380, pp. 451-453
  • Hell, S.W., Far-field optical nanoscopy (2007) S cience, 316, pp. 1153-1158
  • Gustafsson, M.G., Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution (2005) Proc. Natl Acad. Sci. USA, 102, pp. 13081-13086
  • Rust, M.J., Bates, M., Zhuang, X., Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) (2006) Nat. Methods, 3, pp. 793-795
  • Betzig, E., Patterson, G.H., Sougrat, R., Lindwasser, O.W., Olenych, S., Bonifacino, J.S., Davidson, M.W., Hess, H.F., Imaging intracellular fluorescent proteins at nanometer resolution (2006) Science, 313, pp. 1642-1645
  • Hess, S.T., Girirajan, T.P., Mason, M.D., Ultra-high resolution imaging by fluorescence photoactivation localization microscopy (2006) Biophys. J., 91, pp. 4258-4272
  • Sharonov, A., Hochstrasser, R.M., Wide-field subdiffraction imaging by accumulated binding of diffusing probes (2006) Proc. Natl Acad. Sci. USA, 103, pp. 18911-18916
  • Egner, A., Geisler, C., Von Middendorff, C., Bock, H., Wenzel, D., Medda, R., Andresen, M., Hell, S.W., Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters (2007) Biophys. J., 93, pp. 3285-3290
  • Huang, B., Wang, W., Bates, M., Zhuang, X., Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy (2008) Science, 319, pp. 810-813
  • Donnert, G., Keller, J., Medda, R., Andrei, M.A., Rizzoli, S.O., Luhrmann, R., Jahn, R., Hell, S.W., Macromolecular-scale resolution in biological fluorescence microscopy (2006) Proc. Natl Acad. Sci. USA, 103, pp. 11440-11445
  • Sieber, J.J., Willig, K.I., Kutzner, C., Gerding-Reimers, C., Harke, B., Donnert, G., Rammner, B., Lang, T., Anatomy and dynamics of a supramolecular membrane protein cluster (2007) Science, 317, pp. 1072-1076
  • Kellner, R.R., Baier, C.J., Willig, K.I., Hell, S.W., Barrantes, F.J., Nanoscale organization of nicotinic acetylcholine receptors revealed by stimulated emission depletion microscopy (2007) N euroscience, 144, pp. 135-143
  • Kittel, R.J., Wichmann, C., Rasse, T.M., Fouquet, W., Schmidt, M., Schmid, A., Wagh, D.A., Sigrist, S.J., Bruchpilot promotes active zone assembly, Ca 2+ channel clustering, and vesicle release (2006) Science, 312, pp. 1051-1054
  • Westphal, V., Rizzoli, S.O., Lauterbach, M.A., Kamin, D., Jahn, R., Hell, S.W., Video-rate far-field optical nanoscopy dissects synaptic vesicle movement (2008) S cience, 320, pp. 246-249
  • Göppert-Mayer, M., Ü ber Elementarakte mit zwei Quantensprüngen (1931) Ann. Phys., 9, pp. 273-295
  • Denk, W., Strickler, J.H., Webb, W.W., Two-photon laser scanning fluorescence microscopy (1990) Science, 248, pp. 73-76
  • Diaspro, A., Bianchini, P., Vicidomini, G., Faretta, M., Ramoino, P., Usai, C., Multi-photon excitation microscopy (2006) B iomed. Eng. Online, 5, p. 36
  • Diaspro, A., Chirico, G., Collini, M., Two-photon fluorescence excitation and related techniques in biological microscopy (2005) Q. Rev. Biophys., 38, pp. 97-166
  • So, P.T., Dong, C.Y., Masters, B.R., Berland, K.M., Two-photon excitation fluorescence microscopy (2000) Annu. Rev. Biomed. Eng., 2, pp. 399-429
  • Svoboda, K., Yasuda, R., Principles of two-photon excitation microscopy and its applications to neuroscience (2006) N euron, 50, pp. 823-839
  • Bornfleth, H., Edelmann, P., Zink, D., Cremer, T., Cremer, C., Quantitative motion analysis of subchromosomal foci in living cells using four-dimensional microscopy (1999) Biophys. J., 77, pp. 2871-2886
  • Speidel, M., Jonas, A., Florin, E.L., Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging (2003) Opt. Lett., 28, pp. 69-71
  • Prabhat, P., Gan, Z., Chao, J., Ram, S., Vaccaro, C., Gibbons, S., Ober, R.J., Ward, E.S., Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy (2007) Proc. Natl Acad. Sci. USA, 104, pp. 5889-5894
  • Toprak, E., Balci, H., Blehm, B.H., Selvin, P.R., Three-dimensional particle tracking via bifocal imaging (2007) Nanoletters, 7, pp. 2043-2045
  • Kao, H.P., Verkman, A.S., Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position (1994) Biophys. J., 67, pp. 1291-1300
  • Holtzer, L., Meckel, T., Schmidt, T., Nanometric three-dimensional tracking of individual quantum dots in cells (2007) Appl. Phys. Lett., 90, pp. 053902+1-3
  • Saffarian, S., Kirchhausen, T., Differential evanescence nanometry: live-cell fluorescence measurements with 10-nm axial resolution on the plasma membrane (2008) Biophys. J., 94, pp. 2333-2342
  • Sarkar, A., Robertson, R.B., Fernandez, J.M., Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave (2004) Proc. Natl.Acad. Sci. USA, 101, pp. 12882-12886
  • Kis-Petikova, K., Gratton, E., Distance measurement by circular scanning of the excitation beam in the two-photon microscope (2004) Microsc. Res. Tech., 63, pp. 34-49
  • Levi, V., Ruan, Q., Gratton, E., 3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells (2005) Biophys. J., 88, pp. 2919-2928
  • Levi, V., Ruan, Q., Plutz, M., Belmont, A.S., Gratton, E., Chromatin dynamics in interphase cells revealed by tracking in a two-photon excitation microscope (2005) Biophys. J., 89, pp. 4275-4285
  • Enderlein, J., Tracking of fluorescent molecules diffusing within membranes (2000) A ppl. Phys. B, 71, pp. 773-777
  • Berland, K.M., So, P.T., Gratton, E., Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment (1995) Biophys. J., 68, pp. 694-701
  • Lessard, G., Goodwin, P.M., Werner, J.H., Three-dimensional tracking of individual quantum dots (2007) Appl. Phys. Lett., 91 (6), pp. 224106+1-3
  • Vazquez, J., Belmont, A.S., Sedat, J.W., Multiple regimes of constrained chromosome motion are regulated in the interphase Drosophila nucleus (2001) Curr. Biol., 11, pp. 1227-1239
  • Heun, P., Laroche, T., Shimada, K., Furrer, P., Gasser, S.M., Chromosome dynamics in the yeast interphase nucleus (2001) Science, 294, pp. 2181-2186
  • Heard, E., Bickmore, W., The ins and outs of gene regulation and chromosome territory organisation (2007) Curr. Opin. Cell Biol., 19, pp. 311-316
  • Lichter, P., Cremer, T., Borden, J., Manuelidis, L., Ward, D.C., Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries (1988) Hum. Genet., 80, pp. 224-234
  • Cremer, T., Cremer, C., Schneider, T., Baumann, H., Hens, L., Kirsch-Volders, M., Analysis of chromosome positions in the interphase nucleus of Chinese hamster cells by laser-UV-microirradiation experiments (1982) Hum. Genet., 62, pp. 201-209
  • Branco, M.R., Pombo, A., Intermingling of chromosome territories in interphase suggests role in translocations and transcription-dependent associations (2006) PLoS Biol., 4, pp. e138
  • Cremer, T., Cremer, C., Rise, fall and resurrection of chromosome territories: a historical perspective Part I. The rise of chromosome territories (2006) Eur. J. Histochem., 50, pp. 161-176
  • Branco, M.R., Pombo, A., Chromosome organization: new facts, new models (2007) Trends Cell Biol., 17, pp. 127-134
  • Kurz, A., Lampel, S., Nickolenko, J.E., Bradl, J., Benner, A., Zirbel, R.M., Cremer, T., Lichter, P., Active and inactive genes localize preferentially in the periphery of chromosome territories (1996) J. Cell Biol., 135, pp. 1195-1205
  • Chambeyron, S., Da Silva, N.R., Lawson, K.A., Bickmore, W.A., Nuclear re-organisation of the Hoxb complex during mouse embryonic development (2005) Development, 132, pp. 2215-2223
  • Volpi, E.V., Chevret, E., Jones, T., Vatcheva, R., Williamson, J., Beck, S., Campbell, R.D., Sheer, D., Large-scale chromatin organization of the major histocompatibility complex and other regions of human chromosome 6 and its response to interferon in interphase nuclei (2000) J. Cell Sci., 113 (PT 9), pp. 1565-1576
  • Robinett, C.C., Straight, A., Li, G., Willhelm, C., Sudlow, G., Murray, A., Belmont, A.S., Vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition (1996) J. Cell Biol., 135, pp. 1685-1700
  • Belmont, A.S., Li, G., Sudlow, G., Robinett, C., Visualization of large-scale chromatin structure and dynamics using the lac operator/lac repressor reporter system (1999) Methods Cell Biol., 58, pp. 203-222
  • Marshall, W.F., Straight, A., Marko, J.F., Swedlow, J., Dernburg, A., Belmont, A., Murray, A.W., Sedat, J.W., Interphase chromosomes undergo constrained diffusional motion in living cells (1997) Curr. Biol., 7, pp. 930-939
  • Chubb, J.R., Boyle, S., Perry, P., Bickmore, W.A., Chromatin motion is constrained by association with nuclear compartments in human cells (2002) Curr. Biol., 12, pp. 439-445
  • Rosa, A., Maddocks, J.H., Neumann, F.R., Gasser, S.M., Stasiak, A., Measuring limits of telomere movement on nuclear envelope (2006) Biophys. J., 90, pp. L24-L26
  • Tumbar, T., Belmont, A.S., Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator (2001) Nat. Cell Biol., 3, pp. 134-139
  • Hediger, F., Taddei, A., Neumann, F.R., Gasser, S.M., Methods for visualizing chromatin dynamics in living yeast (2004) Methods Enzymol., 375, pp. 345-365
  • Chuang, C.H., Carpenter, A.E., Fuchsova, B., Johnson, T., De Lanerolle, P., Belmont, A.S., Long-range directional movement of an interphase chromosome site (2006) Curr. Biol., 16, pp. 825-831
  • Hellriegel, C., Gratton, E., Real-time multi-parameter spectroscopy and localization in three-dimensional single-particle tracking (2009) J. R. Soc. Interface, 6 (1 SUPPL), pp. S3-S14


---------- APA ----------
Levi, V. & Gratton, E. (2009) . Three-Dimensional Particle Tracking in a Laser Scanning Fluorescence Microscope. Single Particle Tracking and Single Molecule Energy Transfer, 1-24.
---------- CHICAGO ----------
Levi, V., Gratton, E. "Three-Dimensional Particle Tracking in a Laser Scanning Fluorescence Microscope" . Single Particle Tracking and Single Molecule Energy Transfer (2009) : 1-24.
---------- MLA ----------
Levi, V., Gratton, E. "Three-Dimensional Particle Tracking in a Laser Scanning Fluorescence Microscope" . Single Particle Tracking and Single Molecule Energy Transfer, 2009, pp. 1-24.
---------- VANCOUVER ----------
Levi, V., Gratton, E. Three-Dimensional Particle Tracking in a Laser Scanning Fluorescence Microscope. Single Particle Tracking and Single Mol. Energy Transf. 2009:1-24.