Abstract:
Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241-1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2',7-dihidro dicloro fluorescein (H2DCFDA). © Springer Science+Business Media New York 2015. All rights are reserved.
Registro:
| Documento: |
Parte de libro
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| Título: | Improved ROS measurement in root hair cells |
| Autor: | Juárez, S.P.D.; Mangano, S.; Estevez, J.M. |
| Filiación: | IFIByNE (CONICET), FCEyN, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Fisiología, Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina
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| Palabras clave: | 2',7-Dihidro dicloro fluorescein; Root hair; ROS |
| Año: | 2014
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| Página de inicio: | 67
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| Página de fin: | 71
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| DOI: |
http://dx.doi.org/10.1007/978-1-4939-1902-4_6 |
| Título revista: | Plant Cell Expansion: Methods and Protocols
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| Título revista abreviado: | Plant Cell Expans.: Methods and Protoc.
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| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814939_v_n_p67_Juarez |
Referencias:
- Suzuki, N., Miller, G., Morales, J., Shulaev, V., Torres, M., Mittler, R., Respiratory burst oxidases: the engines of ROS signaling (2011) Plant Biol, 14, pp. 691-699
- Roppolo, D., De Rybel, B., Tendon, V., Pfister, A., Alassimone, J., Vermeer, J., Yamazaki, M., Geldner, N., A novel protein family mediates Casparian strip formation in the endodermis (2011) Nature, 473, pp. 380-384
- Takeda, S., Gapper, C., Kaya, H., Bell, E., Kuchitsu, K., Dolan, L., Local positive feedback regulation determines cell shape in root hair cells (2008) Science, 319, pp. 1241-1244
- Potocký, M., Jones, M., Bezvoda, R., Smirnoff, N., Zárský, V., Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth (2007) New Phytol, 174, pp. 742-751
- Foreman, J., Demidchik, V., Bothwell, J., Mylona, P., Miedema, H., Torres, M., Linstead, P., Jones, J., Reactive oxygen species produced by NADPH oxidase regulate plant cell growth (2003) Nature, 422, pp. 442-446
- Karlsson, M., Kurz, T., Brunk, U., Nilsson, S., Frennesson, C., What does the commonly used DCF test for oxidative stress really show? (2010) Biochem J, 428, pp. 183-190
Citas:
---------- APA ----------
Juárez, S.P.D., Mangano, S. & Estevez, J.M.
(2014)
. Improved ROS measurement in root hair cells. Plant Cell Expansion: Methods and Protocols, 67-71.
http://dx.doi.org/10.1007/978-1-4939-1902-4_6---------- CHICAGO ----------
Juárez, S.P.D., Mangano, S., Estevez, J.M.
"Improved ROS measurement in root hair cells"
. Plant Cell Expansion: Methods and Protocols
(2014) : 67-71.
http://dx.doi.org/10.1007/978-1-4939-1902-4_6---------- MLA ----------
Juárez, S.P.D., Mangano, S., Estevez, J.M.
"Improved ROS measurement in root hair cells"
. Plant Cell Expansion: Methods and Protocols, 2014, pp. 67-71.
http://dx.doi.org/10.1007/978-1-4939-1902-4_6---------- VANCOUVER ----------
Juárez, S.P.D., Mangano, S., Estevez, J.M. Improved ROS measurement in root hair cells. Plant Cell Expans.: Methods and Protoc. 2014:67-71.
http://dx.doi.org/10.1007/978-1-4939-1902-4_6