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Borassi, C.; Di Giorgio, J.P.; Scarpin, M.R.; Muschietti, J.; Estevez, J.M. "Optimized method for growing in vitro Arabidopsis thaliana pollen tubes" (2014) Plant Cell Expansion: Methods and Protocols:41-47
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Abstract:

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed. © Springer Science+Business Media New York 2015. All rights are reserved.

Registro:

Documento: Parte de libro
Título:Optimized method for growing in vitro Arabidopsis thaliana pollen tubes
Autor:Borassi, C.; Di Giorgio, J.P.; Scarpin, M.R.; Muschietti, J.; Estevez, J.M.
Filiación:Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Dr. Héctor Torres (INGEBI-CONICET), Buenos Aires, Argentina
Departamento de Fisiología, Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina
Palabras clave:Arabidopsis pollen; Cell expansion; Pollen tube growth
Año:2014
Página de inicio:41
Página de fin:47
DOI: http://dx.doi.org/10.1007/978-1-4939-1902-4_3
Título revista:Plant Cell Expansion: Methods and Protocols
Título revista abreviado:Plant Cell Expans.: Methods and Protoc.
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814939_v_n_p41_Borassi

Referencias:

  • Cheung, A.Y., Hen-ming, W., Structural and signaling networks for the polar cell growth machinery in pollen tubes (2008) Annu Rev Plant Biol, 59, pp. 547-572
  • Cole, R.A., Fowler, J.E., Polarized growth:maintaining focus on the tip (2006) Curr Opin Plant Biol, 9, pp. 579-588
  • Chebli, Y., Geitmann, A., Mechanical principles governing pollen tube growth (2007) Funct Plant Sci Biotechnol, 1, pp. 232-245
  • Boavida, L.C., McCormick, S., Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana (2007) Plant J, 52, pp. 570-582

Citas:

---------- APA ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J. & Estevez, J.M. (2014) . Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. Plant Cell Expansion: Methods and Protocols, 41-47.
http://dx.doi.org/10.1007/978-1-4939-1902-4_3
---------- CHICAGO ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M. "Optimized method for growing in vitro Arabidopsis thaliana pollen tubes" . Plant Cell Expansion: Methods and Protocols (2014) : 41-47.
http://dx.doi.org/10.1007/978-1-4939-1902-4_3
---------- MLA ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M. "Optimized method for growing in vitro Arabidopsis thaliana pollen tubes" . Plant Cell Expansion: Methods and Protocols, 2014, pp. 41-47.
http://dx.doi.org/10.1007/978-1-4939-1902-4_3
---------- VANCOUVER ----------
Borassi, C., Di Giorgio, J.P., Scarpin, M.R., Muschietti, J., Estevez, J.M. Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. Plant Cell Expans.: Methods and Protoc. 2014:41-47.
http://dx.doi.org/10.1007/978-1-4939-1902-4_3