Abstract:
Based on the classical Gram staining technique we created an adapted protocol so it could be used in bacterial suspensions for Gram positive and negative bacteria. The differences between these two types are determined by spectrophotometry. This is a rapid and low cost procedure to classify bacteria. © 2014 IEEE.
Registro:
| Documento: |
Conferencia
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| Título: | Development of an optical sensor for rapid and automatic gram classification of microorganisms |
| Autor: | Ohlsson, A.H.; Bonetto, M.C.; Sacco, N.J.; Corton, E.; Garcia L. |
| Filiación: | Departamento de Química Biológica, IQUIBICEN-CONICET, Ciudad Autónoma de Buenos AiresC1428EGA, Argentina
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| Palabras clave: | bacteria classification; Gram; rapid; sensor; staining; Sensors; Bacterial suspensions; Gram; Gram positive; Gram-staining; Low costs; rapid; staining; Bacteria |
| Año: | 2014
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| DOI: |
http://dx.doi.org/10.1109/IBERSENSOR.2014.6995522 |
| Título revista: | 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014
|
| Título revista abreviado: | IberoAmerican Congr. Sensors, IBERSENSOR - Conf. Proc.
|
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814799_v_n_p_Ohlsson |
Referencias:
- Moyes, R.B., Reynolds, J., Breakwell, D.P., Differential staining of bacteria: Gram stain (2009) Current Protocols in Microbiology, 3 C, pp. 1-8
- Mandigan, M.T., Martinko, J.M., Parker, J., (1997) Brock: Biology of Microorganisms, , 8th ed. Prentice Hall. Upper Saddler River, New Jersey, USA
- Beveridge, T.J., Mechanism of Gram variability in select bacteria (1990) J. Bacteriol., 172, pp. 1609-1620
- Allman, R., Manchee, R., Lloyd, D.D., Flow cytometric analysis of heterogeneous bacterial populations (1993) Flow Cytometry in Microbiology, pp. 27-47. , Springer-Verlag. London, England
- Mason, D.J., Shanmuganathan, S., Mortimer, F.C., Gant, V.A., A fluorescent Gram stain for flow cytometry and epifluorescence microscopy (1998) Appl. Environ. Microbiol, 64, pp. 2681-2685A4 -
Citas:
---------- APA ----------
Ohlsson, A.H., Bonetto, M.C., Sacco, N.J., Corton, E. & Garcia L.
(2014)
. Development of an optical sensor for rapid and automatic gram classification of microorganisms. 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014.
http://dx.doi.org/10.1109/IBERSENSOR.2014.6995522---------- CHICAGO ----------
Ohlsson, A.H., Bonetto, M.C., Sacco, N.J., Corton, E., Garcia L.
"Development of an optical sensor for rapid and automatic gram classification of microorganisms"
. 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014
(2014).
http://dx.doi.org/10.1109/IBERSENSOR.2014.6995522---------- MLA ----------
Ohlsson, A.H., Bonetto, M.C., Sacco, N.J., Corton, E., Garcia L.
"Development of an optical sensor for rapid and automatic gram classification of microorganisms"
. 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014, 2014.
http://dx.doi.org/10.1109/IBERSENSOR.2014.6995522---------- VANCOUVER ----------
Ohlsson, A.H., Bonetto, M.C., Sacco, N.J., Corton, E., Garcia L. Development of an optical sensor for rapid and automatic gram classification of microorganisms. IberoAmerican Congr. Sensors, IBERSENSOR - Conf. Proc. 2014.
http://dx.doi.org/10.1109/IBERSENSOR.2014.6995522