Abstract:
Microinterdigitated electrode structures (μIDES) have widely been used for electrochemical cell impedance spectroscopy (ECIS). For instance ECIS has been used to study biofilm formation and growth of a wide range of microorganisms to detect bacterial infections, food contaminations, biocorrosion, and biofouling. In this study we describe the development of a label-free bioassay to determine the presence of different bacteria strains using ECIS. Following initial characterization of embedded gold μIDES using solutions of increasing conductivities, impedance spectra ranging from 10Hz to 300 kHz in the presence of Bacillus subtilis DMS10 and Shewanella oneidensis MR-1 were recorded over a period of approx. 5 hours. Frequency analysis showed that at 30 Hz the impedance response differences were higher and the relative standard deviations were smaller between each strain and the medium. In contrast to standard cell impedance sensing, which is performed at frequencies higher than 10 kHz to detect cell membrane polarizations, our low frequency results indicate that ionic changes at the electrode-solution double layer (Cdl) resulting by individual bacterial metabolism, can also be used to non-invasively and label-free differentiate microorganisms. © 2014 IEEE.
Registro:
| Documento: |
Conferencia
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| Título: | Rapid and label-free differentiation of bacterial strains using low frequency electrochemical impedance spectroscopy |
| Autor: | Bonetto, M.C.; Sacco, N.J.; Ohlsson, A.H.; Cortón, E.; Sticker, D.; Charwat, V.; Ertl, P.; Garcia L. |
| Filiación: | Biochemistry Department, CONICET-IQUIBICEN, University of Buenos Aires, Buenos Aires, C1428EGA, Argentina
BioSensors Technologies, AIT Austrian Institute of Technology, Muthgasse 11, Vienna, 1190, Austria
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| Palabras clave: | biofilm; interdigitated electrode structures; low frequency electrochemical cell impedance sensing; Bacteria; Bacteriology; Bioassay; Biofilms; Cell membranes; Electrochemical cells; Electrochemical electrodes; Electrodes; Microbiology; Microorganisms; Spectroscopy; Bacterial infections; Bacterial metabolism; Cell impedance; Electrode solutions; Inter-digitated electrodes; Membrane polarization; Relative standard deviations; Shewanella oneidensis MR-1; Electrochemical impedance spectroscopy |
| Año: | 2014
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| DOI: |
http://dx.doi.org/10.1109/IBERSENSOR.2014.6995516 |
| Título revista: | 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014
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| Título revista abreviado: | IberoAmerican Congr. Sensors, IBERSENSOR - Conf. Proc.
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| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814799_v_n_p_Bonetto |
Referencias:
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Citas:
---------- APA ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H., Cortón, E., Sticker, D., Charwat, V., Ertl, P.,..., Garcia L.
(2014)
. Rapid and label-free differentiation of bacterial strains using low frequency electrochemical impedance spectroscopy. 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014.
http://dx.doi.org/10.1109/IBERSENSOR.2014.6995516---------- CHICAGO ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H., Cortón, E., Sticker, D., Charwat, V., et al.
"Rapid and label-free differentiation of bacterial strains using low frequency electrochemical impedance spectroscopy"
. 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014
(2014).
http://dx.doi.org/10.1109/IBERSENSOR.2014.6995516---------- MLA ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H., Cortón, E., Sticker, D., Charwat, V., et al.
"Rapid and label-free differentiation of bacterial strains using low frequency electrochemical impedance spectroscopy"
. 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014, 2014.
http://dx.doi.org/10.1109/IBERSENSOR.2014.6995516---------- VANCOUVER ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H., Cortón, E., Sticker, D., Charwat, V., et al. Rapid and label-free differentiation of bacterial strains using low frequency electrochemical impedance spectroscopy. IberoAmerican Congr. Sensors, IBERSENSOR - Conf. Proc. 2014.
http://dx.doi.org/10.1109/IBERSENSOR.2014.6995516