Abstract:
We recently reported a novel surface architecture for the generation of biocompatible and stable photoswitchable quantum dots (psQDs). The system is based on Förster Resonance Energy Transfer (FRET) from a QD donor to diheteroarylethenes photochromic (PC) acceptors (pcFRET). The PC molecules are linked to an amphiphilic polymer that self-assembles, creating an interior lipophilic space, in which the hydrophobic PCs are included, and external facing carboxyl groups. psQDs retain the desirable properties of the original QDs; furthermore the brightness can be tailored by light. The modulation of emission monitored by steady-state and time-resolved fluorescence is 35-40%. The epidermal growth factor receptor (EGFR) is of far-reaching biomedical importance due to its overexpression and/or mutation in various types of cancer. Here, we demonstrate imaging of live cells exposed to psQDs targeted to the EGFR.
Registro:
| Documento: |
Conferencia
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| Título: | Small photostable photoswitchable quantum dots as nanotools for live cell imaging |
| Autor: | Diaz, S.A.; Toscani, A.M.; Arndt-Jovin, D.J.; Jovin, T.M.; Jares-Erijman, E.A. |
| Ciudad: | Boston, MA |
| Filiación: | Departamento de Química Orgánica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Departamento de Química Biologica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
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| Palabras clave: | EGFR; FRET; Imaging; Photochromic; Quantum dots; Amphiphilic polymers; Carboxyl groups; EGFR; Epidermal growth factor receptors; FRET; Live cell; Live-cell imaging; Nanotools; Novel surfaces; Over-expression; Photo-switchable; Photochromic; Photostable; Quantum Dot; Resonance energy transfer; Time-resolved fluorescence; Energy transfer; Fluidics; Imaging techniques; Medical imaging; Medical nanotechnology; Microcomputers; Nanotechnology; Photochromism; Semiconductor quantum dots |
| Año: | 2011
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| Volumen: | 3
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| Página de inicio: | 205
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| Página de fin: | 208
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| Título revista: | Nanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
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| Título revista abreviado: | Tech. Proc. NSTI Nanotechnology Conf. Expo, NSTI-Nanotech
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| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814398_v3_n_p205_Diaz |
Referencias:
- Walling, M.A., Novak, J.A., Shepard, J.R., (2009) Int J Mol Sci, 10, pp. 441-491
- Medintz, I.L., Mattoussi, H., (2009) Phys Chem Chem Phys, 11, pp. 17-45
- Giordano, L., Jovin, T.M., Irie, M., Jares-Erijman, E.A., (2002) J Am Chem Soc, 124, pp. 7481-7489
- Jares-Erijman, E.A., Jovin, T.M., (2003) Nat Biotechnol, 21, pp. 1387-1395
- Pellegrino, T., Manna, L., Kudera, S., Liedl, T., Koktysh, D., Rogach, A.L., (2004) Nano Lett., 4, pp. 703-707
- Diaz, S.A., Menendez, G.O., Etchehon, M.H., Giordano, L., Jovin, T.M., Jarcs-Erijman, E.A., (2011) ACS Nano, , DOI:10.1021/nn103243c
- Hynes, N.E., MacDonald, G., (2009) Curr Opin Cell Biol, 21, pp. 177-184
- Oda, K., Matsuoka, Y., Funahashi, A., Kitano, H., (2005) Mol Syst Biol, 1, pp. 1-17
- Lidke, D.S., Lidke, K.A., Rieger, B., Jovin, T.M., Arndt-Jovin, D.J., (2005) J Cell Biol, 170, pp. 619-626
- Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Greeco, H.E., (2004) Nat Biotechnol, 22, pp. 198-203
- Sperling, R.A., Parak, W.J., (2010) Philos Transact A Math Phys Eng Sci, 368, pp. 1333-1383
- Hagen, G.M., Caarls, W., Thomas, M., Hill, A., Lidke, K.A., Rieger, B., (2007) Proc. SPIE V, 6441, pp. S4410-S4410A4 - Clean Technology and Sustainable Industries Organization (CTSI); European Patent Office; Greenberg Traurig; Innovation and Materials Science Institute; Jackson Walker L.L.P.
Citas:
---------- APA ----------
Diaz, S.A., Toscani, A.M., Arndt-Jovin, D.J., Jovin, T.M. & Jares-Erijman, E.A.
(2011)
. Small photostable photoswitchable quantum dots as nanotools for live cell imaging. Nanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011, 3, 205-208.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814398_v3_n_p205_Diaz [ ]
---------- CHICAGO ----------
Diaz, S.A., Toscani, A.M., Arndt-Jovin, D.J., Jovin, T.M., Jares-Erijman, E.A.
"Small photostable photoswitchable quantum dots as nanotools for live cell imaging"
. Nanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 3
(2011) : 205-208.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814398_v3_n_p205_Diaz [ ]
---------- MLA ----------
Diaz, S.A., Toscani, A.M., Arndt-Jovin, D.J., Jovin, T.M., Jares-Erijman, E.A.
"Small photostable photoswitchable quantum dots as nanotools for live cell imaging"
. Nanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011, vol. 3, 2011, pp. 205-208.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814398_v3_n_p205_Diaz [ ]
---------- VANCOUVER ----------
Diaz, S.A., Toscani, A.M., Arndt-Jovin, D.J., Jovin, T.M., Jares-Erijman, E.A. Small photostable photoswitchable quantum dots as nanotools for live cell imaging. Tech. Proc. NSTI Nanotechnology Conf. Expo, NSTI-Nanotech. 2011;3:205-208.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814398_v3_n_p205_Diaz [ ]
