Abstract:
Based on synthetic biology tools, a biosensor was designed to be specific for arsenic detection in water. It was designed to be cheap and easy to use (image based instructions will be enough). The device has a modular design which enables us to detect other water pollutants, with minor genetic modifications. In the presence of arsenic, the system develops a red color in a dose dependent manner that can be evaluated by naked eye and compared to an internal standard. Laboratory proof of concept worked nicely and the dynamic range of the assay (between 0 and 500 ppb) covers the critical arsenite concentrations according the World Health Organization. The current effort is focused on building a prototype that could be delivered for domestic use. © 2014 Taylor & Francis Group.
Registro:
| Documento: |
Conferencia
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| Título: | SensAr: An arsenic biosensor for drinking water |
| Autor: | Nadra, A.D.; Basanta, B.; Bonomi, H.; Carlotto, N.; Giménez, M.; Grande, A.; Nieto Moreno, N.; Barone, F.; Dorr, F.; Marasco, L.; Mildiner, S.; Patop, I.; Sosa, S.; Vattino, L.; Vignale, F. |
| Ciudad: | Buenos Aires |
| Filiación: | Departamento de Química Biológica, Facultad de Cs. Exactas Y Naturales, Universidad de Buenos Aires, Argentina
Facultad de Cs. Exactas Y Naturales, Universidad de Buenos Aires, Argentina
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| Palabras clave: | Arsenic; Pollution detection; Water pollution; Arsenic detection; Building a prototypes; Dose-dependent manner; Genetic modifications; Internal standards; Proof of concept; Synthetic biology; World Health Organization; Biosensors |
| Año: | 2014
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| Página de inicio: | 325
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| Página de fin: | 326
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| Título revista: | 5th International Congress on Arsenic in the Environment, As 2014
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| Título revista abreviado: | One Century Discov. Arsenicosis Latin America: As - Proc. Int. Congr. Arsenic Environ.
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| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97811380_v_n_p325_Nadra |
Referencias:
- Alon, U., (2007) An Introduction to Systems Biology: Design Principles of Biological Circuits, pp. 57-64. , Chapman and Hall CRC
- Basu, S., Mehreja, R., Thiberge, S., Chen, M.-T., Weiss, R., Spatiotemporal control of gene expression with pulse-generating networks (2004) PNAS, 101 (17), pp. 6355-6360
- IGEM Competition, , http://igem.org
- Registry of Standard Biological Parts, , http://parts.igem.org/A4 - et al.; National Atomic Energy Commission (CNEA); National Research and Technology Council (CONICET); The Argentine Foundation of Nanotechnology; The National University of Buenos Aires (UBA); The National University of San Martin (UNSAM)
Citas:
---------- APA ----------
Nadra, A.D., Basanta, B., Bonomi, H., Carlotto, N., Giménez, M., Grande, A., Nieto Moreno, N.,..., Vignale, F.
(2014)
. SensAr: An arsenic biosensor for drinking water. 5th International Congress on Arsenic in the Environment, As 2014, 325-326.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97811380_v_n_p325_Nadra [ ]
---------- CHICAGO ----------
Nadra, A.D., Basanta, B., Bonomi, H., Carlotto, N., Giménez, M., Grande, A., et al.
"SensAr: An arsenic biosensor for drinking water"
. 5th International Congress on Arsenic in the Environment, As 2014
(2014) : 325-326.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97811380_v_n_p325_Nadra [ ]
---------- MLA ----------
Nadra, A.D., Basanta, B., Bonomi, H., Carlotto, N., Giménez, M., Grande, A., et al.
"SensAr: An arsenic biosensor for drinking water"
. 5th International Congress on Arsenic in the Environment, As 2014, 2014, pp. 325-326.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97811380_v_n_p325_Nadra [ ]
---------- VANCOUVER ----------
Nadra, A.D., Basanta, B., Bonomi, H., Carlotto, N., Giménez, M., Grande, A., et al. SensAr: An arsenic biosensor for drinking water. One Century Discov. Arsenicosis Latin America: As - Proc. Int. Congr. Arsenic Environ. 2014:325-326.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97811380_v_n_p325_Nadra [ ]
