Artículo
Saavedra, S.L.; Martínez Ceron, M.C.; Giudicessi, S.L.; Forno, G.; Bosco, M.B.; Marani, M.M.; Erra-Balsells, R.; Albericio, F.; Cascone, O.; Camperi, S.A. "Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography" (2018) Biotechnology Progress. 34(4):999-1005
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Abstract:
Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:999–1005, 2018. © 2018 American Institute of Chemical Engineers
Registro:
| Documento: | Artículo |
| Título: | Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography |
| Autor: | Saavedra, S.L.; Martínez Ceron, M.C.; Giudicessi, S.L.; Forno, G.; Bosco, M.B.; Marani, M.M.; Erra-Balsells, R.; Albericio, F.; Cascone, O.; Camperi, S.A. |
| Filiación: | Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Junín 956, Buenos Aires, 1113, Argentina CONICET-Universidad de Buenos Aires, Instituto de Nanobiotecnología (NANOBIOTEC), Facultad de Farmacia y Bioquímica, Junín 956, Buenos Aires, 1113, Argentina R&D Zelltek S.A., UNL, FBCB, Ciudad Universitaria, Paraje el Pozo, CC 242, Santa Fe, Argentina IPEEC-CONICET, Consejo Nacional de Investigaciones Científicas y Técnicas, Bvd. Brown 2915, Puerto Madryn, Chubut 9120, Argentina Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Pabellón II, 3er P., Ciudad Universitaria, Buenos Aires, 1428, Argentina CONICET, Universidad de Buenos Aires, Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Facultad de Ciencias Exactas y Naturales, Pabellón II, 3er P. Ciudad Universitaria, Buenos Aires, 1428, Argentina CIBER-BBN, Networking Centre on Bioengineering, Biomaterials and Nanomedicine, and Department of Organic Chemistry, University of Barcelona, Barcelona, 08028, Spain School of Chemistry and Physics, University of KwaZulu-Natal, Durban, 4001, South Africa |
| Palabras clave: | biologics; downstream processing; peptide ligands; recombinant biopharmaceuticals; solid-phase peptide synthesis; transgenic cow milk; Affinity chromatography; Chelation; Purification; Synthesis (chemical); biologics; Biopharmaceuticals; Cow milk; Downstream-processing; Peptide ligand; Solid phase peptide synthesis; Peptides |
| Año: | 2018 |
| Volumen: | 34 |
| Número: | 4 |
| Página de inicio: | 999 |
| Página de fin: | 1005 |
| DOI: | http://dx.doi.org/10.1002/btpr.2645 |
| Título revista: | Biotechnology Progress |
| Título revista abreviado: | Biotechnol. Prog. |
| ISSN: | 87567938 |
| CODEN: | BIPRE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v34_n4_p999_Saavedra |
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Citas:
---------- APA ----------
Saavedra, S.L., Martínez Ceron, M.C., Giudicessi, S.L., Forno, G., Bosco, M.B., Marani, M.M., Erra-Balsells, R.,..., Camperi, S.A. (2018) . Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography. Biotechnology Progress, 34(4), 999-1005.http://dx.doi.org/10.1002/btpr.2645
---------- CHICAGO ----------
Saavedra, S.L., Martínez Ceron, M.C., Giudicessi, S.L., Forno, G., Bosco, M.B., Marani, M.M., et al. "Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography" . Biotechnology Progress 34, no. 4 (2018) : 999-1005.http://dx.doi.org/10.1002/btpr.2645
---------- MLA ----------
Saavedra, S.L., Martínez Ceron, M.C., Giudicessi, S.L., Forno, G., Bosco, M.B., Marani, M.M., et al. "Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography" . Biotechnology Progress, vol. 34, no. 4, 2018, pp. 999-1005.http://dx.doi.org/10.1002/btpr.2645
---------- VANCOUVER ----------
Saavedra, S.L., Martínez Ceron, M.C., Giudicessi, S.L., Forno, G., Bosco, M.B., Marani, M.M., et al. Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography. Biotechnol. Prog. 2018;34(4):999-1005.http://dx.doi.org/10.1002/btpr.2645
