Artículo

Baieli, M.F.; Urtasun, N.; Martinez, M.J.; Hirsch, D.B.; Pilosof, A.M.R.; Miranda, M.V.; Cascone, O.; Wolman, F.J. "Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey" (2017) Biotechnology Progress. 33(1):171-180
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Casein glycomacropeptide (CMP) is a 64- amino acid peptide found in cheese whey, which is released after κ-casein specific cleavage by chymosin. CMP lacks aromatic amino acids, a characteristic that makes it usable as a nutritional supplement for people with phenylketonuria. CMP consists of two nonglycosylated isoforms (aCMP A and aCMP B) and its different glycosylated forms (gCMP A and gCMP B). The most predominant carbohydrate of gCMP is N-acetylneuraminic acid (sialic acid). Here, we developed a CMP purification process based on the affinity of sialic acid for wheat germ agglutinin (WGA). After formation of chitosan beads and adsorption of WGA, the agglutinin was covalently attached with glutaraldehyde. Two matrices with different WGA density were assayed for CMP adsorption. Maximum adsorption capacities were calculated according to the Langmuir model from adsorption isotherms developed at pH 7.0, being 137.0 mg/g for the matrix with the best performance. In CMP reduction from whey, maximum removal percentage was 79% (specifically 33.7% of gCMP A and B, 75.8% of aCMP A, and 93.9% of aCMP B). The CMP was recovered as an aggregate with an overall yield of 64%. Therefore, the matrices developed are promising for CMP purification from cheese whey. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:171–180, 2017. © 2016 American Institute of Chemical Engineers

Registro:

Documento: Artículo
Título:Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
Autor:Baieli, M.F.; Urtasun, N.; Martinez, M.J.; Hirsch, D.B.; Pilosof, A.M.R.; Miranda, M.V.; Cascone, O.; Wolman, F.J.
Filiación:Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Buenos Aires, Junín 956 (1113), Argentina
Instituto de Nanobiotecnología (NANOBIOTEC), UBA, CONICET, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín, Buenos Aires 956 (1113), Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Industrias, Ciudad Universitaria (1428), Buenos Aires, Argentina
Palabras clave:bovine whey; casein glycomacropeptide; chitosan; chromatography matrix; wheat germ agglutinin; Adsorption; Affinity chromatography; Amino acids; Carboxylic acids; Chitin; Chitosan; Chromatography; Mammals; Purification; Adsorption capacities; Aromatic amino acid; Bovine whey; Casein glycomacropeptide; N-acetylneuraminic acid; Nutritional supplements; Purification process; Wheat germ agglutinin; Casein; amino acid; casein; caseinomacropeptide; chitosan; n acetylneuraminic acid; peptide fragment; whey protein; adsorption; affinity chromatography; animal; bovine; chemistry; glycosylation; isolation and purification; metabolism; milk; whey; Adsorption; Amino Acids; Animals; Caseins; Cattle; Chitosan; Chromatography, Affinity; Glycosylation; Milk; N-Acetylneuraminic Acid; Peptide Fragments; Whey; Whey Proteins
Año:2017
Volumen:33
Número:1
Página de inicio:171
Página de fin:180
DOI: http://dx.doi.org/10.1002/btpr.2404
Título revista:Biotechnology Progress
Título revista abreviado:Biotechnol. Prog.
ISSN:87567938
CODEN:BIPRE
CAS:amino acid, 65072-01-7; casein, 9000-71-9; chitosan, 9012-76-4; n acetylneuraminic acid, 131-48-6; Amino Acids; caseinomacropeptide; Caseins; Chitosan; N-Acetylneuraminic Acid; Peptide Fragments; Whey Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v33_n1_p171_Baieli

Referencias:

  • Brody, E.P., Biological activities of bovine glycomacropeptide (2000) Br J Nutr., 84, pp. 39-46
  • Abd El-Salam, M.H., Separation of casein glycomacropeptide from whey: Methods of potential industrial application (2006) Int J Dairy Sci., 1, pp. 93-99
  • Mollé, D., Léonil, J., Quantitative determination of bovine k-casein macropeptide in dairy products by Liquid chromatography/Electrospray coupled to mass spectrometry (LC-ESI/MS) and Liquid chromatography/Electrospray coupled to tandem mass spectrometry (LC-ESI/MS/MS) (2005) Int Dairy J., 15, pp. 419-428
  • Martinez, M.J., Farías, M.E., Pilosof, A.M.R., Casein glycomacropeptide pH-driven self-assembly and gelation upon heating (2011) Food Hydrocoll., 25, pp. 860-867
  • Nakano, T., Ozimek, L., Purification of glycomacropeptide from dialyzed and non-dialyzed sweet whey by anion-exchange chromatography at different pH values (2000) Biotechnol. Lett., 22, pp. 1081-1086
  • Nakano, T., Ikawa, N., Ozimek, L., Use of epichlorohydrin-treated chitosan resin as an adsorbent to isolate kappa-casein glycomacropeptide from sweet whey (2004) J Agric Food Chem., 52, pp. 7555-7560
  • Thoma-Worringer, C., Sorensen, J., López-Fandiño, R., Health effects and technological features of caseinomacropeptide (2006) Int Dairy J., 16, pp. 1324-1333
  • Silva Hernández, E.R., Herrera Meza, M.S., Verdalet Guzmán, I., Herrera Lee, R.G., Nakano, T., Ozimek, L., (2005) Aislamiento y caracterización de glicomacropéptido (GMP) y suero libre de GMP y sus usos potenciales como nutracéuticos. V Congreso Iberoamericano de Ingeniería de Alimentos, , (CIBIA)., México
  • Harish Prashanth, K.V., Tharanathan, R.N., Chitin/chitosan: modifications and their unlimited application potential—an overview (2007) Trends Food Sci Technol., 18, pp. 117-131
  • Senstad, C., Mattiasson, B., Purification of wheat germ agglutinin using affinity flocculation with chitosan and a subsequent centrifugation or flotation step (1989) Biotechnol Bioeng., 34, pp. 387-393
  • Dutta, P.K., Dutta, J., Tripathi, V.S., Chitin and chitosan: chemistry, properties and applications (2004) J Sci Ind Res., 63, pp. 20-31
  • Zeng, X., Ruckenstein, E., Control of pore sizes in macroporous chitosan and chitin membranes (1996) Ind Eng Chem Res., 35, pp. 4169-4175
  • Wolman, F.J., Copello, G.J., Mebert, A.M., Targovnik, A.M., Miranda, M.V., Navarro del Cañizo, A.A., Díaz, L.E., Cascone, O., Egg white lysozyme purification with a chitin–silica-based affinity chromatographic matrix (2010) Eur Food Res Technol., 231, pp. 181-188
  • Terbojevich, M., Muzzarelli, R.A.A., (2000) Handbook of Hydrocolloids, pp. 367-378. , Chitosan. In, Philips GO, Wiliams PA, editors., Cambridge, UK, Woodhead Publishing
  • Nagata, Y., Burger, M.M., Wheat germ agglutinin. Molecular characteristics and specificity for sugar binding (1974) J Biol Chem., 249, pp. 3116-3122
  • Zeng, X., Ruckenstein, E., Macroporous chitin affinity membranes for wheat germ agglutinin purification from wheat germ (1999) J Membr Sci., 156, pp. 97-107
  • Nagahora, H., Ishikawa, K., Niwa, Y., Muraki, M., Jigami, Y., Expression and secretion of wheat germ agglutinin by Saccharomyces cerevisiae (1992) Eur J Biochem., 210, pp. 989-997
  • Monzo, A., Bonn, G.K., Guttman, A., Lectin-immobilization strategies for affinity purification and separation of glycoconjugates (2007) TrAC Trends Anal Chem., 26, pp. 423-432
  • Chu, L., Macloud, A., Ozimek, L., Isolation of glycomacropeptide from sodium caseinate hydrolysate solution by ultrafiltration (1996) Milchwiss., 51, pp. 303-306
  • Kawasaki, Y., Dawakami, H., Tanimoto, M., Dosako, S., Tomizawa, A., Kotake, M., Nakajima, I., pH-dependent molecular weigth changes of k-casein glycomacropeptide and its preparation by ultrafiltration (1993) Milchwiss., 48, pp. 191-195
  • Nakano, T., Ozimek, L., Isolation of glycomacropeptide from sweet whey by gel chromatography on Sephacryl S-200, at pH 7.0 and 3.5 (2002) Milchwiss., 57, pp. 128-130
  • Doultani, S., Turhan, K.N., Etzel, M.R., Whey protein isolate and glyco-macropeptide recovery from whey using ion exchange chromatography (2003) J Food Sci., 68, pp. 1389-1395
  • Thomä, C., Krause, I., Kulozik, U., Precipitation behaviour of caseinomacropeptides and their simultaneous determination with whey proteins by RP-HPLC (2006) Int Dairy J., 16, pp. 285-293
  • Farías, M.E., Martinez, M.J., Pilosof, A.M.R., Casein glycomacropeptide pH-dependent self-assembly and cold gelation (2010) Int Dairy J., 20, pp. 79-88
  • Baieli, M.F., Urtasun, N., Miranda, M.V., Cascone, O., Wolman, F.J., Efficient wheat germ agglutinin purification with a chitosan-based affinity chromatographic matrix (2012) J Sep Sci., 35, pp. 231-238
  • Guo, T.-Y., Xia, Y.-Q., Hao, G.-J., Zhang, B.-H., Fu, G.-Q., Yuan, Z., He, B.-L., Kennedy, J.F., Chemically modified chitosan beads as matrices for adsorptive separation of proteins by molecularly imprinted polymer (2005) Carbohydr Polym., 62, pp. 214-221
  • Roh, I.J., Kwon, I.C., Fabrication of a pure porous chitosan bead matrix: influences of phase separation on the microstructure (2002) J Biomater Sci: Polym Ed, 13, pp. 769-782
  • Akkuş, Ç.Ş., Nursevin, Ö.H., Immobilization of catalase into chemically crosslinked chitosan beads (2003) Enzyme Microb Technol., 32, pp. 889-894
  • Nakano, T., Ozimek, L., Purification of glycomacropeptide from non-dialyzable fraction of sweet whey by hydrophobic interaction chromatography on phenyl-agarose (2000) Biotechnol Lett., 22, pp. 413-416
  • Chase, H.A., Prediction of the performance of preparative affinity chromatography (1984) J Chromatogr., 297, pp. 179-202
  • Martinez, M.J., Carrera Sánchez, C., Rodríguez Patino, J.M., Pilosof, A.M.R., Interactions in the aqueous phase and adsorption at the air-water interface of caseinoglycomacropeptide (GMP) and b-lactoglobulin mixed systems (2009) Coll Surf B: Biointerfaces., 68, pp. 39-47
  • Fukuda, S.P., Roig, S.M., Prata, L.F., Correlation between acidic ninhydrin and HPLC methods to evaluate fraudulent addition of whey in milk (2004) Lait., 84, pp. 501-512
  • Zeng, X., Ruckenstein, E., Cross-linked macroporous chitosan anion-exchange membranes for protein separations (1998) J Membr Sci., 148, pp. 195-205
  • Kreub, M., Strixner, T., Kulozik, U., The effect of glycosylation on the interfacial properties of bovine caseinomacropeptide (2009) Food Hydrocoll., 23, pp. 1818-1826
  • Kristiansen, A., Nysæter, Å., Grasdalen, H., Vårum, K.M., Quantitative studies of the binding of wheat germ agglutinin (WGA) to chitin-oligosaccharides and partially N-acetylated chitosans suggest inequivalence of binding sites (1999) Carbohydr Polym., 38, pp. 23-32
  • JP-Selecta, S.A., http://www.grupo-selecta.com/notasdeaplicaciones/analisis-alimentarios-y-de-aguas-nutritional-and-water-analysis/determinacion-de-proteinas-por-el-metodo-de-kjeldahl-kjeldahl-method-for-protein-determination/, Determinación de proteínas por el método de Kjeldahl/Kjeldahl method for protein determination, June, 2011. Accessed June 09, 2016; Galindo-Amaya, L.L., Valbuena-Colmenares, E., Rojas-Villarroel, E., Standardization of glycomacropeptide detection with SDS–PAGE as a milk adulteration index (2006) Rev Cient. (Maracaibo), 16, pp. 308-314
  • Tullio, L.T., Lazzari Karkle, E.N., Cândido, L.M.B., (2007) Revisão: isolamento e purificação do glicomacropeptídeo do soro de leite [Review: isolation and purification of glycomacropeptide the serum of milk], 25, pp. 121-132. , Boletim do Centro de Pesquisa de Processamento de Alimentos, Brazil
  • Tanimoto, M., Kawasaki, Y., Shinmoto, H., Dosako, S., Tomizawa, A., Process for producing kappa-casein glycomacropeptide. U.S. 075,424, 1991; Kawasaki, Y., Dosako, S., (1994), Process for producing kappa-glycomacropeptide. U.S. 278-288; Outinen, M., Tossavianen, O., Syvaoja, E.L., Korhonen, H., Chromatographic isolation of κ-casein macropeptide from cheese whey with a strong basic anion exchange resin (1995) Milchwiss., 50, pp. 570-574
  • Etzel, M.R., (2001), Production of substantially pure kappa casein macropeptide. U.S. 168-823

Citas:

---------- APA ----------
Baieli, M.F., Urtasun, N., Martinez, M.J., Hirsch, D.B., Pilosof, A.M.R., Miranda, M.V., Cascone, O.,..., Wolman, F.J. (2017) . Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey. Biotechnology Progress, 33(1), 171-180.
http://dx.doi.org/10.1002/btpr.2404
---------- CHICAGO ----------
Baieli, M.F., Urtasun, N., Martinez, M.J., Hirsch, D.B., Pilosof, A.M.R., Miranda, M.V., et al. "Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey" . Biotechnology Progress 33, no. 1 (2017) : 171-180.
http://dx.doi.org/10.1002/btpr.2404
---------- MLA ----------
Baieli, M.F., Urtasun, N., Martinez, M.J., Hirsch, D.B., Pilosof, A.M.R., Miranda, M.V., et al. "Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey" . Biotechnology Progress, vol. 33, no. 1, 2017, pp. 171-180.
http://dx.doi.org/10.1002/btpr.2404
---------- VANCOUVER ----------
Baieli, M.F., Urtasun, N., Martinez, M.J., Hirsch, D.B., Pilosof, A.M.R., Miranda, M.V., et al. Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey. Biotechnol. Prog. 2017;33(1):171-180.
http://dx.doi.org/10.1002/btpr.2404