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Abstract:

Polyethylene glycol (PEG)-based low generation dendrimers are analyzed as single excipient or combined with trehalose in relation to their structure and efficiency as enzyme stabilizers during freeze-thawing, freeze-drying, and thermal treatment. A novel functional dendrimer (DGo-CD) based on the known PEG's ability as cryo-protector and β-CD as supramolecular stabilizing agent is presented. During freeze-thawing, PEG and β-CD failed to prevent catalase denaturation, while dendrimers, and especially DGo-CD, offered the better protection to the enzyme. During freeze-drying, trehalose was the best protective additive but DGo-CD provided also an adequate catalase stability showing a synergistic behavior in comparison to the activities recovered employing PEG or β-CD as unique additives. Although all the studied dendrimers improved the enzyme remaining activity during thermal treatment of freeze-dried formulations, the presence of amorphous trehalose was critical to enhance enzyme stability. The crystallinity of the protective matrix, either of PEG derivatives or of trehalose, negatively affected catalase stability in the freeze-dried systems. When humidified at 52% of relative humidity, the dendrimers delayed trehalose crystallization in the combined matrices, allowing extending the protection at those conditions in which normally trehalose fails. The results show how a relatively simple covalent combination of a polymer such as PEG with β-CD could significantly affect the properties of the individual components. Also, the results provide further insights about the role played by polymer-enzyme supramolecular interactions (host-guest crosslink, hydrogen bonding, and hydrophobic interactions) on enzyme stability in dehydrated models, being the effect on the stabilization also influenced by the physical state of the matrix. © 2013 American Institute of Chemical Engineers Biotechnol. © 2013 American Institute of Chemical Engineers.

Registro:

Documento: Artículo
Título:Polyethylene glycol-based low generation dendrimers functionalized with β-cyclodextrin as cryo- and dehydro-protectant of catalase formulations
Autor:Santagapita, P.R.; Mazzobre, M.F.; Cruz, A.G.; Corti, H.R.; Villalonga, R.; Buera, M.P.
Filiación:Industry Dept. and Organic Chemistry Dept., Faculty of Exact and Natural Sciences, University of Buenos Aires, Intendente Güiraldes 2160, Argentina
Ciudad Universitaria-(FCEyN-UBA), And National Council of Scientific and Technical Research (CONICET), C1428EGA-Ciudad Autónoma de Buenos Aires, Argentina
Centro de Estudios de Tecnología Enzimática, Facultad de Agronomía, Universidad de Matanzas Camilo Cienfuegos, Autopista a Varadero km 31/2 (44740), Matanzas, Cuba
Departamento de Física de la Materia Condensada, Comisión Nacional de Energía Atómica, Centro Atómico Constituyentes, Avda. General Paz 1499, San Martín, 1650, Argentina
Instituto de Química Física de los Materiales, Ambiente y Energía (INQUIMAE), Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires, Ciudad Universitaria, Intendente Güiraldes 2160, 1428, Buenos Aires, Argentina
Dept. of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Av. de Séneca 2, 28040, Madrid, Spain
Palabras clave:β-cyclodextrin; Catalase; Dehydration; Dendrimer; Enzyme stability; Freezing and thawing, PEG; Trehalose; Catalase; Enzyme stability; Freeze-dried formulations; Freezing and thawing; Hydrophobic interactions; Polyethylene glycol (PEG); Supramolecular interactions; Trehalose; Additives; Dehydration; Enzyme activity; Heat treatment; Hydrogen bonds; Low temperature drying; Models; Polyethylene glycols; Stabilization; Supramolecular chemistry; Thawing; Dendrimers; beta cyclodextrin derivative; catalase; dendrimer; excipient; macrogol derivative; trehalose; analysis of variance; article; chemistry; drug effect; enzyme stability; freeze drying; hydrodynamics; particle size; temperature; Analysis of Variance; beta-Cyclodextrins; Catalase; Dendrimers; Enzyme Stability; Excipients; Freeze Drying; Hydrodynamics; Particle Size; Polyethylene Glycols; Temperature; Trehalose
Año:2013
Volumen:29
Número:3
Página de inicio:786
Página de fin:795
DOI: http://dx.doi.org/10.1002/btpr.1713
Título revista:Biotechnology Progress
Título revista abreviado:Biotechnol. Prog.
ISSN:87567938
CODEN:BIPRE
CAS:catalase, 9001-05-2; trehalose, 99-20-7; Catalase, 1.11.1.6; Dendrimers; Excipients; Polyethylene Glycols; Trehalose, B8WCK70T7I; beta-Cyclodextrins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v29_n3_p786_Santagapita

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Citas:

---------- APA ----------
Santagapita, P.R., Mazzobre, M.F., Cruz, A.G., Corti, H.R., Villalonga, R. & Buera, M.P. (2013) . Polyethylene glycol-based low generation dendrimers functionalized with β-cyclodextrin as cryo- and dehydro-protectant of catalase formulations. Biotechnology Progress, 29(3), 786-795.
http://dx.doi.org/10.1002/btpr.1713
---------- CHICAGO ----------
Santagapita, P.R., Mazzobre, M.F., Cruz, A.G., Corti, H.R., Villalonga, R., Buera, M.P. "Polyethylene glycol-based low generation dendrimers functionalized with β-cyclodextrin as cryo- and dehydro-protectant of catalase formulations" . Biotechnology Progress 29, no. 3 (2013) : 786-795.
http://dx.doi.org/10.1002/btpr.1713
---------- MLA ----------
Santagapita, P.R., Mazzobre, M.F., Cruz, A.G., Corti, H.R., Villalonga, R., Buera, M.P. "Polyethylene glycol-based low generation dendrimers functionalized with β-cyclodextrin as cryo- and dehydro-protectant of catalase formulations" . Biotechnology Progress, vol. 29, no. 3, 2013, pp. 786-795.
http://dx.doi.org/10.1002/btpr.1713
---------- VANCOUVER ----------
Santagapita, P.R., Mazzobre, M.F., Cruz, A.G., Corti, H.R., Villalonga, R., Buera, M.P. Polyethylene glycol-based low generation dendrimers functionalized with β-cyclodextrin as cryo- and dehydro-protectant of catalase formulations. Biotechnol. Prog. 2013;29(3):786-795.
http://dx.doi.org/10.1002/btpr.1713