Artículo
Abstract:
A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pregrowth in lactose. The evolution of acid production in a continuous flow-stopped flow bioreactor was monitored for different sugar solutions in contact with the immobilized bacteria. The resulting slopes (ΔmV/Δt) were used to quantify the fermentation capability for a defined sugar related to that of glucose, which was taken as 100%. The procedure is simple, being based on pH variation that can give quantitative results compared to other reported techniques for carbohydrate fermentation pattern from which only qualitative results are obtained. In addition, it offers reduction in time and costs and is a suitable tool for the rapid analysis of isolated strains and in studies of modifications of sugar metabolism in mutants. A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pregrowth in lactose. The evolution of acid production in a continuous flow-stopped flow bioreactor was monitored for different sugar solutions in contact with the immobilized bacteria. The resulting slopes (ΔmV/Δt) were used to quantify the fermentation capability for a defined sugar related to that of glucose, which was taken as 100%. The procedure is simple, being based on pH variation that can give quantitative results compared to other reported techniques for carbohydrate fermentation pattern from which only qualitative results are obtained. In addition, it offers reduction in time and costs and is a suitable tool for the rapid analysis of isolated strains and in studies of modifications of sugar metabolism in mutants.
Registro:
| Documento: | Artículo |
| Título: | Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique |
| Autor: | Cortón, E.; Piuri, M.; Battaglini, F.; Ruzal, S.M. |
| Filiación: | Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 4 piso, (1428) Buenos Aires, Argentina Departamento de Quimica Inorganica, Analitica y Quimica Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (1428) Buenos Aires, Argentina |
| Palabras clave: | Calcium alginate; Catabolite; Lactobacillus; Lactose; Monosaccharides; bacterial mutant; biomass; bioreactor; carbohydrate; catabolite repression; colony forming unit; electrode; fermentation; immobilized biomass; immobilized cell culture; lactose; pH; quantitative analysis; Bacteria; Biomass; Bioreactors; Calcium compounds; Carbohydrates; Cell immobilization; Glucose; Growth kinetics; Metabolism; pH; Reduction; Fermentation; Biomass; Bioreactors; Biotechnology; Carbohydrate Metabolism; Cells, Immobilized; Fermentation; Hydrogen-Ion Concentration; Lactobacillus; Lactobacillus casei; Species Specificity; Bacteria (microorganisms); Lactobacillus |
| Año: | 2000 |
| Volumen: | 16 |
| Número: | 1 |
| Página de inicio: | 59 |
| Página de fin: | 63 |
| DOI: | http://dx.doi.org/10.1021/bp9901217 |
| Título revista: | Biotechnology Progress |
| Título revista abreviado: | Biotechnol. Prog. |
| ISSN: | 87567938 |
| CODEN: | BIPRE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v16_n1_p59_Corton |
Referencias:
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Citas:
---------- APA ----------
Cortón, E., Piuri, M., Battaglini, F. & Ruzal, S.M. (2000) . Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique. Biotechnology Progress, 16(1), 59-63.http://dx.doi.org/10.1021/bp9901217
---------- CHICAGO ----------
Cortón, E., Piuri, M., Battaglini, F., Ruzal, S.M. "Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique" . Biotechnology Progress 16, no. 1 (2000) : 59-63.http://dx.doi.org/10.1021/bp9901217
---------- MLA ----------
Cortón, E., Piuri, M., Battaglini, F., Ruzal, S.M. "Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique" . Biotechnology Progress, vol. 16, no. 1, 2000, pp. 59-63.http://dx.doi.org/10.1021/bp9901217
---------- VANCOUVER ----------
Cortón, E., Piuri, M., Battaglini, F., Ruzal, S.M. Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique. Biotechnol. Prog. 2000;16(1):59-63.http://dx.doi.org/10.1021/bp9901217
