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Abstract:

The glass transition temperature (T(g)) of preparations of the restriction enzyme EcoRI, vacuum-dried in the presence of sucrose, trehalose, or raffinose, was determined using differential scanning calorimetry. T(g) values were well below those expected for low-moisture sucrose, trehalose, or raffinose, and this was attributed to the presence of glycerol (a plasticizer), which was a main component of the restriction enzyme preparation. This was verified by determining the glass transition temperature of glycerol, which was found to be (onset value) -77 °C. Present results confirmed that vitrification (i.e., glass formation) was not necessary for enzyme protection in present low-moisture saccharide systems. As shown in previous work, enzyme EcoRI was very stable stored at 37/45 °C in spite of the fact that sugar matrices were completely rubbery, as unequivocally demonstrated in the present work. The glass transition temperature (Tg) of preparations of the restriction enzyme EcoRI, vacuum-dried in the presence of sucrose, trehalose, or raffinose, was determined using differential scanning calorimetry. Tg values were well below those expected for low-moisture sucrose, trehalose, or raffinose, and this was attributed to the presence of glycerol (a plasticizer), which was a main component of the restriction enzyme preparation. This was verified by determining the glass transition temperature of glycerol, which was found to be (onset value) -77°C. Present results confirmed that vitrification (i.e., glass formation) was not necessary for enzyme protection in present low-moisture saccharide systems. As shown in previous work, enzyme EcoRI was very stable stored at 37/45°C in spite of the fact that sugar matrices were completely rubbery, as unequivocally demonstrated in the present work.

Registro:

Documento: Artículo
Título:DSC confirmation that vitrification is not necessary for stabilization of the restriction enzyme EcoRI dried with saccharides
Autor:Buera, M.P.; Rossi, S.; Moreno, S.; Chirife, J.
Ciudad:New York, NY, United States
Filiación:Departamento de Industrias, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:enzyme stability; restriction endonuclease ecori; vitrification; Drying; Enzyme kinetics; Glass transition; Moisture; Plasticizers; Polysaccharides; Sugar (sucrose); Temperature measurement; Vacuum applications; Vitrification; Low moisture saccharide systems; Raffinose; Restriction enzyme; Trehalose; Differential scanning calorimetry
Año:1999
Volumen:15
Número:3
Página de inicio:577
Página de fin:579
DOI: http://dx.doi.org/10.1021/bp990032u
Título revista:Biotechnology Progress
Título revista abreviado:Biotechnol. Prog.
ISSN:87567938
CODEN:BIPRE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v15_n3_p577_Buera

Referencias:

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Citas:

---------- APA ----------
Buera, M.P., Rossi, S., Moreno, S. & Chirife, J. (1999) . DSC confirmation that vitrification is not necessary for stabilization of the restriction enzyme EcoRI dried with saccharides. Biotechnology Progress, 15(3), 577-579.
http://dx.doi.org/10.1021/bp990032u
---------- CHICAGO ----------
Buera, M.P., Rossi, S., Moreno, S., Chirife, J. "DSC confirmation that vitrification is not necessary for stabilization of the restriction enzyme EcoRI dried with saccharides" . Biotechnology Progress 15, no. 3 (1999) : 577-579.
http://dx.doi.org/10.1021/bp990032u
---------- MLA ----------
Buera, M.P., Rossi, S., Moreno, S., Chirife, J. "DSC confirmation that vitrification is not necessary for stabilization of the restriction enzyme EcoRI dried with saccharides" . Biotechnology Progress, vol. 15, no. 3, 1999, pp. 577-579.
http://dx.doi.org/10.1021/bp990032u
---------- VANCOUVER ----------
Buera, M.P., Rossi, S., Moreno, S., Chirife, J. DSC confirmation that vitrification is not necessary for stabilization of the restriction enzyme EcoRI dried with saccharides. Biotechnol. Prog. 1999;15(3):577-579.
http://dx.doi.org/10.1021/bp990032u