Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

ABSTRACT: Similar localizations of the Con A and wheat germ lectin receptors were obtained by using fluorescent lectins, in nonfixed spermatozoa and in spermatozoa fixed with formaldehyde and methanol, showing that in samples with the same previous treatment, worked out by the same operator, in which enough determinations have been performed to eliminate individual variations, the different procedures of fixation produced similar results. The localizations obtained with fluorescent lectins confirm previous results, produced with the peroxidase technique, indicating that the lectins interact with oligomannosidic oligosaccharide receptors situated mainly in the equatorial segment of the acrosome and postnu‐clear cap. They also indicate the presence of similar receptors that were not detected previously on the neck and intermediate segment. The larger size of the lectin‐peroxidase‐diaminebenzidine reagent compared to that of the fluorescent lectins suggests that the new receptors are semicriptic and were not detected by steric effects in the first case, but were able to interact with lower volume, fluorescent probes. It is suggested that these oligomannosidic chains could be recognition signals for the elimination of incompetent sperm during their passage through the female reproductive track. Also these oligosaccharides and its possible metabolic variations could be involved in the interaction between the acrosome‐reacted spermatozoa with the zone pellucida. 1985 Munksgaard

Registro:

Documento: Artículo
Título:Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures
Autor:NARDONE, P.; CEREZO, A.S.; DE CEREZO, J.M.S.
Filiación:Centro de Investigaciones En Reproducción, Facultad de Medicina
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:cytochemical localization; human spermatozoa; oligomannosidic oligosaccharides; lectin; oligosaccharide; cytochemistry; cytology; human; male genital system; mannose oligosaccharide; oligosaccharide receptor; priority journal; spermatozoon; Comparative Study; Concanavalin A; Fixatives; Fluoresceins; Formaldehyde; Histocytochemistry; Human; Lectins; Male; Methanol; Receptors, Concanavalin A; Receptors, Mitogen; Spermatozoa; Support, Non-U.S. Gov't
Año:1985
Volumen:9
Número:4
Página de inicio:124
Página de fin:128
DOI: http://dx.doi.org/10.1111/j.1600-0897.1985.tb00291.x
Título revista:American Journal of Reproductive Immunology and Microbiology
Título revista abreviado:Am. J. Reprod. Immunol. Microbiol.
ISSN:87558920
CAS:Concanavalin A, 11028-71-0; Fixatives; fluorescein isothiocyanate-concanavalin A; fluorescein isothiocyanate-wheat germ agglutinin; Fluoresceins; Formaldehyde, 50-00-0; Lectins; Methanol, 67-56-1; Receptors, Concanavalin A; Receptors, Mitogen; wheat germ agglutinin receptor
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87558920_v9_n4_p124_NARDONE

Referencias:

  • Van Lis, YMG, Kalsbeek, GL., The interaction between human spermatozoa and the lectin concanavalin A (1973) Andrologia, 5, pp. 45-57
  • Uhlenbruck, G, Herrman, WP., Agglutination of normal, coated and enzyme treated human spermatozoa with heterophile agglutinins (1972) Vox Sang, 23, pp. 444-451
  • Edelman, GM, Millete, C., Molecular probes of spermatozoa structures (Fluorescence probes/sperm motility/agglutination/lectins/ subcellular fractions) (1971) Proc. Natl. Acad. Sci. U.S.A, 18, pp. 2436-2440
  • Oikawa, T, Nicolson, GL, Yanagimachi, R., Inhibition of hamster fertilization by phyto agglutinins (1974) Exp. Cell. Res, 83, pp. 239-246
  • de Cerezo, JMS, Bueno, MP, Skowroñski, B, Cerezo, AS., Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma (1982) J. Reprod. Immunol, 4, pp. 11-21
  • de Cerezo, JMS, Bueno, MP, Skowroñski, B, Cerezo, AS., Immunohistochemical localization of Concanavalin A and Wheat Germ lectin receptors in the normal human spermatozoa (1982) Am. J. Reprod. Immunol, 2, pp. 246-249
  • Boettcher, B, Hjort, T, Rümke, P, Shulman, S, Vyazoo, E., Auto e isoantibodies to antigens of the human reproductive system. I. Results of an international comparative study. WHO reference bank for reproductive immunology (1977) Clin. Exp. Immunol, 30, pp. 173-180
  • Goldstein, IJ, Hayes, CE., Carbohydrate bendings proteins of plants and animals (1973) Adv. Carbohydr. Chem. Biochem, 35, pp. 128-334
  • Montreuil, J., Primary structure of glycoprotein in glycans (1980) Adv. Carbohydr. Chem. Biochem, 37, pp. 157-223
  • Beyer, TA, Hill, RL., Glycosylation pathways in the biosynthesis of non reducing terminal sequences in oligosaccharides of glycoproteins (1982) The glycoconjugates, 3, pp. 25-45. , Academic Press, Inc
  • Day, JF, Thornbing, RW, Thorpe, SR, Baynes, JW., Carbohydrate‐mediate clearance of antibody‐antigen complexes from the circulation (1980) J. Biol. Chem, 255, pp. 2360-2365
  • Yanagimachi, R, Phillips, DM., The status of acrosomal caps of hamster spermatozoa immediately before fertilization in vivo (1984) Gamete Research, 9, pp. 1-19
  • Yanagimachi, R, Noda, YD., Ultrastructural changes in the hamster sperm head during fertilization (1970) J. Ultrastruct. Res, 31, pp. 465-485
  • Bedford, JM, Moore, HDM, Franklin, LE., Significance of the equatorial segment of the acrosome of the spermatozoa in entherian mammals (1979) Exp. Cell. Res, 119, pp. 119-126
  • Yanagimachi, R, Okada, A, Tung, KSK, Sperm autoantigens and fertilization: II. Effects of anti‐guinea pig sperm autoantibodies on sperm‐ovum interactions (1981) Biol. Reprod, 24, pp. 512-518

Citas:

---------- APA ----------
NARDONE, P., CEREZO, A.S. & DE CEREZO, J.M.S. (1985) . Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures. American Journal of Reproductive Immunology and Microbiology, 9(4), 124-128.
http://dx.doi.org/10.1111/j.1600-0897.1985.tb00291.x
---------- CHICAGO ----------
NARDONE, P., CEREZO, A.S., DE CEREZO, J.M.S. "Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures" . American Journal of Reproductive Immunology and Microbiology 9, no. 4 (1985) : 124-128.
http://dx.doi.org/10.1111/j.1600-0897.1985.tb00291.x
---------- MLA ----------
NARDONE, P., CEREZO, A.S., DE CEREZO, J.M.S. "Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures" . American Journal of Reproductive Immunology and Microbiology, vol. 9, no. 4, 1985, pp. 124-128.
http://dx.doi.org/10.1111/j.1600-0897.1985.tb00291.x
---------- VANCOUVER ----------
NARDONE, P., CEREZO, A.S., DE CEREZO, J.M.S. Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures. Am. J. Reprod. Immunol. Microbiol. 1985;9(4):124-128.
http://dx.doi.org/10.1111/j.1600-0897.1985.tb00291.x