Artículo

Marín-Briggiler, C.I.; Vazquez-Levin, M.H.; Gonzalez-Echevarría, F.; Blaquier, J.A.; Miranda, P.V.; Tezón, J.G. "Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction" (2003) American Journal of Reproductive Immunology. 50(3):209-219
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Abstract:

Problem: To determine the ability of IgGs isolated from follicular fluids (hFFIgGs) to induce the acrosome reaction (AR) in human spermatozoa and to inhibit sperm-zona pellucida (ZP) interaction. Method of study: Incubation of capacitated spermatozoa with hFFIgGs (n = 40) and assessment of their effect on the AR or hemizona (HZ) assay in a condition that allows sperm-ZP interaction, avoiding acrosomal exocytosis. Results: hFFIgGs from different women varied in their ability of inducing the AR. Those hFFIgGs with the highest AR-inducing capacity evoked the exocytotic response in most of the different sperm donors tested [high Induction Frequency (IF)]. Some of these antibodies were also able of inhibiting sperm binding to ZP [low HZ Index (HZI)]. A significant correlation was found between the IF and the HZI for each hFFIgG. Conclusions: Human follicular fluid contains antibodies capable of inducing the AR and inhibiting sperm-ZP binding, suggesting that they could be directed towards ZP receptors. hFFIgGs would constitute a tool for the identification of sperm entities involved in fertilization. © Blackwell Munksgaard, 2003.

Registro:

Documento: Artículo
Título:Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction
Autor:Marín-Briggiler, C.I.; Vazquez-Levin, M.H.; Gonzalez-Echevarría, F.; Blaquier, J.A.; Miranda, P.V.; Tezón, J.G.
Filiación:Inst. de Bio. y Med. Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Fertilab, Riobamba, Buenos Aires, Argentina
Palabras clave:Acrosome reaction; Antisperm antibodies; Follicular fluid; Spermatozoa; Zona pellucida; immunoglobulin G; sperm antibody; acrosome reaction; adult; antibody isolation; article; cell interaction; clinical article; controlled study; correlation analysis; exocytosis; female; fertilization in vitro; human; human cell; immunoassay; infertility; inhibition kinetics; intracytoplasmic sperm injection; male; ovary follicle fluid; priority journal; scoring system; sperm donor; spermatozoon capacitation; Acrosome Reaction; Adult; Female; Follicular Fluid; Humans; Immunoglobulin G; Male; Spermatozoa; Zona Pellucida
Año:2003
Volumen:50
Número:3
Página de inicio:209
Página de fin:219
DOI: http://dx.doi.org/10.1034/j.1600-0897.2003.00082.x
Título revista:American Journal of Reproductive Immunology
Título revista abreviado:Am. J. Reprod. Immunol.
ISSN:87558920
CODEN:AAJID
CAS:immunoglobulin G, 97794-27-9; Immunoglobulin G
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87558920_v50_n3_p209_MarinBriggiler

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Citas:

---------- APA ----------
Marín-Briggiler, C.I., Vazquez-Levin, M.H., Gonzalez-Echevarría, F., Blaquier, J.A., Miranda, P.V. & Tezón, J.G. (2003) . Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction. American Journal of Reproductive Immunology, 50(3), 209-219.
http://dx.doi.org/10.1034/j.1600-0897.2003.00082.x
---------- CHICAGO ----------
Marín-Briggiler, C.I., Vazquez-Levin, M.H., Gonzalez-Echevarría, F., Blaquier, J.A., Miranda, P.V., Tezón, J.G. "Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction" . American Journal of Reproductive Immunology 50, no. 3 (2003) : 209-219.
http://dx.doi.org/10.1034/j.1600-0897.2003.00082.x
---------- MLA ----------
Marín-Briggiler, C.I., Vazquez-Levin, M.H., Gonzalez-Echevarría, F., Blaquier, J.A., Miranda, P.V., Tezón, J.G. "Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction" . American Journal of Reproductive Immunology, vol. 50, no. 3, 2003, pp. 209-219.
http://dx.doi.org/10.1034/j.1600-0897.2003.00082.x
---------- VANCOUVER ----------
Marín-Briggiler, C.I., Vazquez-Levin, M.H., Gonzalez-Echevarría, F., Blaquier, J.A., Miranda, P.V., Tezón, J.G. Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction. Am. J. Reprod. Immunol. 2003;50(3):209-219.
http://dx.doi.org/10.1034/j.1600-0897.2003.00082.x