Artículo

Barañao, R.I.; Piazza, A.; Rumi, L.S.; De Fried, E.P. "Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media" (1997) American Journal of Reproductive Immunology. 37(2):191-194
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Abstract:

Problem: The aim of this study was to evaluate the presence of interleukin-1 (IL-1) and IL-6 in 24-hour human embryo culture-conditioned media (HECCM) and to find any embryo-related factor(s) to predict pregnancy during IVF procedure. Method: IL-1β and IL-6 levels were measured in 36 samples of HECCM and 17 cell culture media that had not been exposed to embryos, which were used as controls. Both cytokine levels were measured by the ELISA technique, using commercially available kits. Results and Conclusions: We found an average IL-1β level ± SEM of 82 ± 4 pg/ml (n = 11) in HECCM from viable pregnancies and a significantly lower value, 14 ± 2 pg/ml (n = 25, P < 0.001), in HECCM from embryos that did not lead to viable pregnancies. In control media the average IL-1β level was 11 ± 1 pg/ml (n = 17), (P < 0.001 vs. HECCM from viable pregnancies). In contrast IL-6 levels were undetectable in all samples analyzed. Judging by our results we suggest that measurement of IL-1 level in HECCM could be a useful parameter for predicting implantation in techniques of assisted reproduction.

Registro:

Documento: Artículo
Título:Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media
Autor:Barañao, R.I.; Piazza, A.; Rumi, L.S.; De Fried, E.P.
Filiación:Inst. de Biol./Med. Experimental, CONICET, Obligado 2490, 1428 Buenos Aires, Argentina
Centro de Salud Reproductiva (CER), Humboldt 2267, 1425 Buenos Aires, Argentina
Inst. de Biol./Med. Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Human embryo cultured conditioned media (HECCM); IL-6; Interleukin-1 (IL-1); Viable pregnancies; interleukin 1beta; interleukin 6; adult; article; clinical article; controlled study; culture medium; embryo; embryo culture; enzyme linked immunosorbent assay; female; fertilization in vitro; human; human cell; nidation; pregnancy; priority journal
Año:1997
Volumen:37
Número:2
Página de inicio:191
Página de fin:194
DOI: http://dx.doi.org/10.1111/j.1600-0897.1997.tb00212.x
Título revista:American Journal of Reproductive Immunology
Título revista abreviado:AM. J. REPROD. IMMUNOL.
ISSN:87558920
CODEN:AAJID
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87558920_v37_n2_p191_Baranao

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Citas:

---------- APA ----------
Barañao, R.I., Piazza, A., Rumi, L.S. & De Fried, E.P. (1997) . Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media. American Journal of Reproductive Immunology, 37(2), 191-194.
http://dx.doi.org/10.1111/j.1600-0897.1997.tb00212.x
---------- CHICAGO ----------
Barañao, R.I., Piazza, A., Rumi, L.S., De Fried, E.P. "Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media" . American Journal of Reproductive Immunology 37, no. 2 (1997) : 191-194.
http://dx.doi.org/10.1111/j.1600-0897.1997.tb00212.x
---------- MLA ----------
Barañao, R.I., Piazza, A., Rumi, L.S., De Fried, E.P. "Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media" . American Journal of Reproductive Immunology, vol. 37, no. 2, 1997, pp. 191-194.
http://dx.doi.org/10.1111/j.1600-0897.1997.tb00212.x
---------- VANCOUVER ----------
Barañao, R.I., Piazza, A., Rumi, L.S., De Fried, E.P. Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media. AM. J. REPROD. IMMUNOL. 1997;37(2):191-194.
http://dx.doi.org/10.1111/j.1600-0897.1997.tb00212.x