Navegar

Colección

Datos Estadísticas

Artículo

MAZZINI, M.N.; CERACI, P.; DE CEREZO, J.M.S.; CEREZO, A.S. "Carbohydrates of the Surface of the Normal Human Spermatozoon" (1986) American Journal of Reproductive Immunology and Microbiology. 11(4):107-111
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

ABSTRACT: Autoproteolysis of human spermatozoa produces oligopeptides with oligosaccharide chains of the N‐glycosidic‐linked type that are released from the “surface exposed” parts of glycoproteins. The products eluted in the previous washing of the spermatozoa have the same composition and solubility characteristics as the oligopeptides from the digestion. This suggests that autoproteolysis is a constant process that normally occurs on the spermatozoa membrane. The cytochemical characterization and localization of the N‐glycosidic‐linked oligosaccharide receptors on the human spermatozoa membrane after digestion, in the presence or absence of seminal plasma, indicates that only part of the oligosaccharides are cleaved. Their distribution on the different zones of the spermatozoon changed as the probability of detecting these receptors in the intermediate segment increased after proteolysis; this indicates that in this zone the receptors are cryptic ones that become exposed by the action of the proteolytic enzymes. In the presence of seminal plasma most receptors on the acrosome are eliminated. 1986 Munksgaard

Registro:

Documento: Artículo
Título:Carbohydrates of the Surface of the Normal Human Spermatozoon
Autor:MAZZINI, M.N.; CERACI, P.; DE CEREZO, J.M.S.; CEREZO, A.S.
Filiación:Centro de Investigationes en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Argentina
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:autoproteolysis; human spermatozoa; N‐Glycosidic‐linked oligosaccharides; glycoprotein; receptor; biological model; human; human cell; male genital system; priority journal; protein degradation; spermatozoon; Carbohydrates; Cell Membrane; Human; Male; Membrane Proteins; Oligopeptides; Oligosaccharides; Spermatozoa; Support, Non-U.S. Gov't
Año:1986
Volumen:11
Número:4
Página de inicio:107
Página de fin:111
DOI: http://dx.doi.org/10.1111/j.1600-0897.1986.tb00042.x
Título revista:American Journal of Reproductive Immunology and Microbiology
Título revista abreviado:Am. J. Reprod. Immunol. Microbiol.
ISSN:87558920
CAS:Carbohydrates; Membrane Proteins; Oligopeptides; Oligosaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87558920_v11_n4_p107_MAZZINI

Referencias:

  • de Cerezo, JMS, Bueno, MP, Skowronsky, B, Cerezo, AS., Immunohistochemical localization of Con A and wheat germ lectin receptors in the normal human spermatozoa (1982) Am J Reprod Immunol, 2, pp. 246-249
  • de Cerezo, JMS, Bueno, MP, Skowroñsky, B, Cerezo, AS., Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma (1982) J Reprod Immunol, 4, pp. 11-21
  • Tortorella, H, de Cerezo, JMS, Mazzini, MN, Cerezo, AS., The antigenic mucin‐like glycoproteins of human seminal plasma (1983) Am J Reprod Immunol, 4, pp. 76-82
  • Propping, D, Tauber, PF, Zaneveld, LJD, An improved assay technique for the proteolytic activity of individual human spermatozoa (1978) Int J Fertil, 23, pp. 45-50
  • Benítez‐Bribiesca, L, Velázquez‐Meza, S., Cytochemcial demonstration of proteolytic activity of human and rat spermatozoa (1972) J Reprod Fertil, 29, pp. 419-420
  • Kalb, VT, Bernlohr, RW., A new spectrophotometric assay for protein in cell extracts (1977) Anal Biochem, 82, pp. 362-371
  • Warburg, B, Christian, W., Protein estimation by ultraviolet absorption (1957) Meth Enzymol, 3, pp. 451-454
  • Dubois, M, Gilles, KA, Hamilton, JK, Rebers, A, Smith, F., Colorimetric method for determination of sugars and related substances (1956) Anal Chem, 28, pp. 350-356
  • Reinhold, VN., Gas liquid chromatographic analysis of constituent carbohydrates in glycoproteins (1972) Meth Enzymol, 25, pp. 244-249
  • Mazzini, MN, Cerezo, AS., The carbohydrate and protein composition of the endosperm, embryo and testa of the seed of Gleditsia triacanthos (1979) Journal of the Science of Food and Agriculture, 30, pp. 881-891
  • Berger, L, Broida, D., The quantitative colorimetric determination in serum and urine of leucine aminopeptidase (1975) Sigma Technical Bulletin, 251, pp. 4-5
  • Scacciati, JM, Mancini, RE., Soluble and insoluble antigens of human spermatozoa (1975) Fertil Steril, 26, pp. 6-12
  • Quinn, PJ, White, IG, Cleland, KW., Chemical and ultrastructural changes in ram spermatozoa after washing, cold shock and freezing (1969) J Reprod Fertil, 18, pp. 209-220
  • Montreuil, J., Primary structure of glycoprotein glycans (1980) Adv Carbohyd Chem Biochem, 37, pp. 157-223
  • Nasir‐Ud‐Din, Walker‐Nasir, E, Jeanloz, RW., Isolation and identification of spermatozoon‐surface glycoproteins from Macaca radiata (1980) Carbohydrate Research, 85, p. C7
  • Nasir‐Ud‐Din, Walker‐Nasir, E, Ajaz, S, Malghani, MA., (1984) Sperm‐surface glycoproteins: Isolation, purification and partial characterization, p. 249. , Abstracts of the XIIth International Carbohydrate Symposium, Vliegerithart Utrecht JFG, Karuberlinig JP, Veldirek GA, Vorik Publishers, The Netherlands
  • Beyer, TA, Hill, RL., Glycosidation pathways in the biosynthesis of non‐reducing terminal sequences in oligosaccharides of glycoproteins (1982) The glycoconjugates, 3, p. 25. , Horowitz MI, Pigman W, New York:, Academic Press
  • Mettler, L, Skrabei, H., Isolation of human spermatozoa membrane antigen binding sperm‐immobilizing and sperm‐agglutinating antibodies (1979) Int. J. Fertil, 24, pp. 44-48
  • Hayry, P, Defeudi, V., Surface antigens of SU40, transformed tumor cells (1970) Virology, 41, pp. 22-29

Citas:

---------- APA ----------
MAZZINI, M.N., CERACI, P., DE CEREZO, J.M.S. & CEREZO, A.S. (1986) . Carbohydrates of the Surface of the Normal Human Spermatozoon. American Journal of Reproductive Immunology and Microbiology, 11(4), 107-111.
http://dx.doi.org/10.1111/j.1600-0897.1986.tb00042.x
---------- CHICAGO ----------
MAZZINI, M.N., CERACI, P., DE CEREZO, J.M.S., CEREZO, A.S. "Carbohydrates of the Surface of the Normal Human Spermatozoon" . American Journal of Reproductive Immunology and Microbiology 11, no. 4 (1986) : 107-111.
http://dx.doi.org/10.1111/j.1600-0897.1986.tb00042.x
---------- MLA ----------
MAZZINI, M.N., CERACI, P., DE CEREZO, J.M.S., CEREZO, A.S. "Carbohydrates of the Surface of the Normal Human Spermatozoon" . American Journal of Reproductive Immunology and Microbiology, vol. 11, no. 4, 1986, pp. 107-111.
http://dx.doi.org/10.1111/j.1600-0897.1986.tb00042.x
---------- VANCOUVER ----------
MAZZINI, M.N., CERACI, P., DE CEREZO, J.M.S., CEREZO, A.S. Carbohydrates of the Surface of the Normal Human Spermatozoon. Am. J. Reprod. Immunol. Microbiol. 1986;11(4):107-111.
http://dx.doi.org/10.1111/j.1600-0897.1986.tb00042.x