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Abstract:

Peptides from skin secretions of amphibians are considered important components of their immune system and also play a relevant role in their defense mechanism against predators. Herein, by using mass spectrometry (MS), we characterize the sequence of 13 peptides from the gland secretion of the hylid tree frog, Boana punctata. Using in situ matrix-assisted laser desorption ionization imaging MS of a transverse section of the skin tissue, we show that some peptides are stored as longer molecules that are cleaved after being secreted, whereas others do not undergo any modification. Sequence comparison with peptides from other Boana species and analysis of the three-dimensional theoretical structure indicate that this cleavage depends on both the presence of a specific sequence motif and the secondary structure. The fact that peptides undergo a rapid cleavage upon secretion suggests that stored and secreted peptides may have distinct roles for anuran survival, including defense against pathogens and predators. Copyright © 2018 American Chemical Society.

Registro:

Documento: Artículo
Título:Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry
Autor:Brunetti, A.E.; Marani, M.M.; Soldi, R.A.; Mendonça, J.N.; Faivovich, J.; Cabrera, G.M.; Lopes, N.P.
Filiación:Faculty of Pharmaceutical Sciences of Ribeirão Preto, Department of Physics and Chemistry, University of São Paulo, Avenida do Café, s/no, Ribeirão Preto, 14040-903, Brazil
Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 3° piso, Buenos Aires, C1428EHA, Argentina
División Herpetología, Museo Argentino de Ciencias Naturales Bernardino Rivadavia, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenue Angel Gallardo 490, Ciudad de Buenos Aires, C1405DJR, Argentina
IPEEC - CONICET, Consejo Nacional de Investigaciones Científicas y Técnicas, Bvd. Brown 2915, Puerto Madryn, U9120ACD, Argentina
Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 4° piso, Ciudad de Buenos Aires, C1428EHA, Argentina
Facultad de Ciencias Exactas y Naturales, Unidad de Microanalisis y Metodos Fisicos Aplicados A la Quimica Organica, CONICET - Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 3° piso, Buenos Aires, C1428EHA, Argentina
Año:2018
Volumen:3
Número:5
Página de inicio:5426
Página de fin:5434
DOI: http://dx.doi.org/10.1021/acsomega.7b02029
Título revista:ACS Omega
Título revista abreviado:ACS Omega
ISSN:24701343
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_24701343_v3_n5_p5426_Brunetti

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Citas:

---------- APA ----------
Brunetti, A.E., Marani, M.M., Soldi, R.A., Mendonça, J.N., Faivovich, J., Cabrera, G.M. & Lopes, N.P. (2018) . Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry. ACS Omega, 3(5), 5426-5434.
http://dx.doi.org/10.1021/acsomega.7b02029
---------- CHICAGO ----------
Brunetti, A.E., Marani, M.M., Soldi, R.A., Mendonça, J.N., Faivovich, J., Cabrera, G.M., et al. "Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry" . ACS Omega 3, no. 5 (2018) : 5426-5434.
http://dx.doi.org/10.1021/acsomega.7b02029
---------- MLA ----------
Brunetti, A.E., Marani, M.M., Soldi, R.A., Mendonça, J.N., Faivovich, J., Cabrera, G.M., et al. "Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry" . ACS Omega, vol. 3, no. 5, 2018, pp. 5426-5434.
http://dx.doi.org/10.1021/acsomega.7b02029
---------- VANCOUVER ----------
Brunetti, A.E., Marani, M.M., Soldi, R.A., Mendonça, J.N., Faivovich, J., Cabrera, G.M., et al. Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry. ACS Omega. 2018;3(5):5426-5434.
http://dx.doi.org/10.1021/acsomega.7b02029