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Abstract:

Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva. © 2018 The Authors

Registro:

Documento: Artículo
Título:Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
Autor:Medina, R.; Lucentini, C.G.; Franco, M.E.E.; Petroselli, G.; Rosso, J.A.; Erra-Balsells, R.; Balatti, P.A.; Saparrat, M.C.N.
Filiación:Centro de Investigaciones en Fitopatologías, CIDEFI, Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICBA), Departamento de Ciencias Biológicas, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, CC 31, La Plata, 1900, Argentina
Centro de Investigaciones en Hidratos de Carbono, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 3 Ciudad Universitaria, Buenos Aires, 1428, Argentina
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Diag. 113 y 64, CC 16 suc. 4, La Plata, 1900, Argentina
Cátedra de Microbiología Agrícola, Departamento de Ciencias Biológicas, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, La Plata, 1900, Argentina
Instituto de Fisiología Vegetal (INFIVE), Consejo Nacional de Investigaciones Científicas y técnicas (CONICET), Universidad Nacional de La Plata CCT-La Plata, Diag. 113 y 61, CC 327, La Plata, 1900, Argentina
Instituto de Botánica Carlos Spegazzini, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, 53 # 477, La Plata, 1900, Argentina
Palabras clave:Biochemistry; Genetics; Microbiology; Molecular biology
Año:2018
Volumen:4
Número:12
DOI: http://dx.doi.org/10.1016/j.heliyon.2018.e01036
Título revista:Heliyon
Título revista abreviado:Heliyon
ISSN:24058440
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_24058440_v4_n12_p_Medina

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Citas:

---------- APA ----------
Medina, R., Lucentini, C.G., Franco, M.E.E., Petroselli, G., Rosso, J.A., Erra-Balsells, R., Balatti, P.A.,..., Saparrat, M.C.N. (2018) . Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum). Heliyon, 4(12).
http://dx.doi.org/10.1016/j.heliyon.2018.e01036
---------- CHICAGO ----------
Medina, R., Lucentini, C.G., Franco, M.E.E., Petroselli, G., Rosso, J.A., Erra-Balsells, R., et al. "Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)" . Heliyon 4, no. 12 (2018).
http://dx.doi.org/10.1016/j.heliyon.2018.e01036
---------- MLA ----------
Medina, R., Lucentini, C.G., Franco, M.E.E., Petroselli, G., Rosso, J.A., Erra-Balsells, R., et al. "Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)" . Heliyon, vol. 4, no. 12, 2018.
http://dx.doi.org/10.1016/j.heliyon.2018.e01036
---------- VANCOUVER ----------
Medina, R., Lucentini, C.G., Franco, M.E.E., Petroselli, G., Rosso, J.A., Erra-Balsells, R., et al. Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum). Heliyon. 2018;4(12).
http://dx.doi.org/10.1016/j.heliyon.2018.e01036